1. bioconversion of progesterone using aqueous two phase system by comamonas acidovorans mtcc 3364
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Strains of Comamonas acidovoras have been ...
Strains of Comamonas acidovoras have been
reported to have vital role in degradation of natural
as well as complex organic compounds.
Comamonas acidovoras MTCC 3364 has been
routinely reported for steroid bioconversion by
activated beads in aqueous system. Previously
studies observed that progesterone was converted
into AD and ADD steroids. These compounds were
higher valuable steroids which were mainly used in
different types of drugs. Novel system was used for
the bioconversion of progesterone in to AD and
ADD. Aqueous two-phase system was initially
optimized for the progesterone solubility, separation
of two phases and extraction of product steroids
from the system. Even 1% of PEG can easily
dissolved progesterone but 5% of PEG needed for
separating phases and for extraction purposes.
Beads was easily tolerate up to 20% of PEG system
even for 120 hours. Hexane used as better
extraction solvent among different type of solvent
system. Products were shown in PEG 6000 system
after 96 hours in fewer amounts. PEG 8000 system
showed products 48 hours in minor amount but
increased in 96 hours. Advantage of aqueous twophase
system in progesterone bioconversion was
better stability of progesterone, extraction of steroid
products and stability of the system.
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