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seminar ppt

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  • 1. Identification of Genes required for Cytoplasmic Localization in Early C. elegans Embryos
  • 2. Background
  • 3. Development of C.elegans
  • 4. Development
    • After fertilization, the two pronucleis join together.
    • http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
  • 5. Development
    • Then the mitotic spindle forms, and it migrates posteriorly.
    • http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
  • 6. Development
    • Owing to the migration, the two daughter blastomeres are produced in different sizes after the first cleavage.
    • http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
  • 7. Development
    • At the second cleavage, AB divides transversely and P 1 divides longitudinally. AB always divides before P 1 .
    • http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
  • 8. Principles of Development (Lewis Wholper) Chapter 5
  • 9.
    • After fertilization, the two pronucleis join together.
    • Mitotic spindle forms, and it migrates posteriorly.
    • Daughter blastomeres are produced in different sizes after the first cleavage.
    • AB > P1
    • P granules are localized to P1
    • At the second cleavage, AB divides transversely and P1 divides longitudinally.
    • AB always divides before P1
    • P granules are localized to P2
  • 10.
    • After fertilization, the two pronucleis join together.
    • Mitotic spindle forms, and it migrates posteriorly.
    • Daughter blastomeres are produced in different sizes after the first cleavage.
    • AB > P1
    • P granules are localized to P1
    • At the second cleavage, AB divides transversely and P1 divides longitudinally .
    • AB always divides before P1
    • P granules are localized to P2
  • 11. The Result
  • 12. Genes
  • 13. Defects
    • Equal First Cleavage
    • Altered Orientation of Second Cleavages
    • Synchronous Cleavages
    • Abnormal Localization of P Granule
  • 14. Aim
    • To identify the genes required for cytoplasmic localization
    • in early C. elegans embryos
  • 15. Sub-Questions
  • 16.
    • http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/research-details.cfm
  • 17. Identify The Genes
    • Screening
    • There are four categories of mutation, but three of them
    • are hard to observe.
    • So they chose the forth.
    • Linkage Mapping
    • Linkage Analysis
    • Meiotic Recombination
    • Complementation
  • 18. Identify The Defects
    • Equal First Cleavage
    • Altered Orientation of Second Cleavages
    • Synchronous Cleavages
    • Abnormal Localization of P Granule
  • 19. Equal First Cleavage
  • 20. Equal First Cleavage
    • Blastomere Size Measurement
    • Spindle Movement Measurement
  • 21. Equal First Cleavage
  • 22. Equal First Cleavage
  • 23.  
  • 24.  
  • 25. Altered Second Cleavages
  • 26. Altered Second Cleavages
  • 27. Altered Second Cleavages
  • 28. Synchronous Cleavages
  • 29. Synchronous Cleavages
    • par-1 and par-4 cleave synchronously up until fourth or fifth cleavage
    • par-2 and par-3 are rather earlier
  • 30. Abnormal Localization of P Granule
  • 31. Abnormal Localization of P Granule
  • 32. Abnormal Localization of P Granule
  • 33. Identify The Phenotype
    • Differentiated Cell Types
    • Grandchildless
  • 34. The Result
  • 35. Summary