Cytochrome P450 genes in the
Polistes dominula genome
Daniel Standage, Brendel Group Meeting, 19 Nov 2013


Motivation
 Differential expression analysis in paper wasp
 Initially found 123 differentially expressed gene loci
 Man...
Cytochrome P450 (CYP)
 Oxidation enzyme
 RH + O2 + NAD(P)H + H+  ROH + H2O + NADP+

 Found in most (all) organisms
 B...
CYPs in Insects
 1,744 distinct insect CYPs (Feb 2010)
 +/- 100 CYP genes per species common
 CYPs functions in insects...
CYPs in social insects
 Functions not well characterized
 CYPs often associated with juvenile hormone (JH)
 JH, ecdyson...
CYPs in Solenopsis invicta
 Subtractive hybridization to identify DE genes
 Found 3 genes, 2 of which are CYPs
 Develop...
CYPs in H. sjostedti
 Identified by differential display
 Expression induced by JH analogue
 42% AA identity with CYP6X...
CYPs in R. flavipes
 Goal: sample CYP4 diversity with degenerate PCR
 Identified seven novel CYP4 genes
 JH-induced dif...
Brendel Group Presentation: 19 Nov 2013
Upcoming SlideShare
Loading in …5
×

Brendel Group Presentation: 19 Nov 2013

73
-1

Published on

Slides from my literature review.

Published in: Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
73
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
2
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Brendel Group Presentation: 19 Nov 2013

  1. 1. Cytochrome P450 genes in the Polistes dominula genome Daniel Standage, Brendel Group Meeting, 19 Nov 2013 
  2. 2. Motivation  Differential expression analysis in paper wasp  Initially found 123 differentially expressed gene loci  Manual examination found many CYP genes  Results pending  BLAST2GO enrichment test  HyCGD
  3. 3. Cytochrome P450 (CYP)  Oxidation enzyme  RH + O2 + NAD(P)H + H+  ROH + H2O + NADP+  Found in most (all) organisms  Broad functional spectrum  “Substrate promiscuity”  High copy number
  4. 4. CYPs in Insects  1,744 distinct insect CYPs (Feb 2010)  +/- 100 CYP genes per species common  CYPs functions in insects  Degradation of exogenous chemical compounds  Odorants  Sex pheromones  Toxins and xenobiotics  Synthesis / degradation of endogenous compounds  Juvenile hormone  Ecdysone
  5. 5. CYPs in social insects  Functions not well characterized  CYPs often associated with juvenile hormone (JH)  JH, ecdysone, and vitellogenin implicated in caste determination  DE analysis has identified 3+ CYP genes with caste- dependent differential expression
  6. 6. CYPs in Solenopsis invicta  Subtractive hybridization to identify DE genes  Found 3 genes, 2 of which are CYPs  Developmentally regulated  Caste-specifically regulated
  7. 7. CYPs in H. sjostedti  Identified by differential display  Expression induced by JH analogue  42% AA identity with CYP6X1; named CYP6AM1  CYP6AM1 repressed after JHA treatment
  8. 8. CYPs in R. flavipes  Goal: sample CYP4 diversity with degenerate PCR  Identified seven novel CYP4 genes  JH-induced differentiation influences expression
  1. A particular slide catching your eye?

    Clipping is a handy way to collect important slides you want to go back to later.

×