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Arturo Brenes' presentation in the framework of the expert consultation on the use of crop wild relatives for pre-breeding in potato
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Arturo Brenes' presentation in the framework of the expert consultation on the use of crop wild relatives for pre-breeding in potato

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The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the......

The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.

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  • 1. Arturo Brenes Angulo Centro de Investigaciones Agronómicas Universidad de Costa RicaCIP- Global Crop Diversity Trust; February 22-24, 2012, Lima, Perú
  • 2. Solanum laxissimum S. berthaultii
  • 3. Costa Rica 51 100 Km2 (0.03% of the planet’s surface) ≈ 4,2 Millions Habitants 4% of the total species estimated worldwide (more than 500 000 species )
  • 4. The potato and its wild relatives;section Tuberarium of the genusSolanum.by Donovan Stewart Correll 1962
  • 5. According to Correll (1962), in Costa Rica existe 8 potato wildrelatives- S. juglandifolium y S. oxycarpum of the subsectión Hyperbasarthrum- S. canense, S. caripense, S. fraxinifolium, S. muricatum (introduced),S. suaveolens y S. taeniotrichum of the subsección Basarthrum
  • 6. Collecting, conservation ,characterization and use of the potato wild relatives from Costa Rica
  • 7. Solanum woodsonii
  • 8. Solanum longiconicum
  • 9. Solanum taenotrichium
  • 10. Solanum canense
  • 11. Solanum caripense
  • 12. Solanum fraxinifolium
  • 13. Wild Potato Relatives collected in Costa Rica Species No of accessionsSolanum canense Ruydb.: 2Solanum caripense Humb.&Bonpl.Ex.Dun.: 10Solanum fraxinifolium Dunal in DC: 7Solanum longiconicum Bitter 12Solanum taenotrichum Corr.: 3Solanum woodsonii Corr.: 11 Total 45
  • 14. Leafminer (Liriomyza spp.)
  • 15. Source: www.ecohuellas.files.wordpress.com/.../sequia1.jpg
  • 16. Phenotyping for Drought Stress
  • 17. Chromosome Chromosome Number PCR Marker Resistance to Number PCR Marker Resistance to I CP108 E. carotovora ssp. atroseptica VIII ND Rpi-blb1, P.infestans II GP23 E. carotovora ssp. atroseptica VIII ND Osm8, III GP1 (a) P.infestans VI ND Rpi-blb2, P.infestans III GP25 SSCP/TaqI (CAPS) Taq1 PVY, P.infestans IV ND Rpi-blb3, P.infestans III Ssp8 Gro1.4, G. rostochiensis IX GP94 (SSCP-Hinf I/Dpn I? Rpi-phu1, P.infestans III GP276 P.infestans IX Prp1 Prp1, PRP, P.infestans IV Stm3016 Gpa4, G. pallida, P. infestans IX InvGF-4d Tuber starch IX Gp6 QTL for G. pallida IX InvGF-4b Chip color V BA47f2 P.infestans XI GP250 R3, R6, R7, P. infestans V CM1 H1, G. rostochiensis XI NL25 (SSCP) Sen1, Sinchrytium endobioticum V CosA P.infestans XI NL27 (3 and 5, Borman et al, 2003) PLRV.1, PLRV V CP113 HI, G.rostochiensis XI NL27 SCAR (Marzewski et al, 2001) PLRV.1, PLRV V R1general P.infestans XI RYSC3 (SCAR) Ryadg, PVY V R1specific P.infestans XI CP58 Ryadg, Rysto, PVY, Sen1 Ssp72 GP125 (CAPS) Taq1 V G.pallida XI PVY,Sen1, P.infestans, V TG69 Gro VI , G.rostochiensis XI TG523 Rysto,Na adg,RMc1, GP21 R1, Rx2, NB, Gpa,GPa5, Grp1,P. infestans, PVX,G. Ssp75 V pallida, G. rostochiensis XI Gro1.3, G.rostochiensis GP179 R1,GPa5, Grp1,P. infestans, PVX,G. pallida,G. TG30 V rostochiensis XI Gro1.3, G.rostochiensis GP76 GP185 R3, R6, R7, P. infestans , VI P. infestans XI E.carotovora ssp. atroseptica VI GP79 P. infestans XI Osm11, VI St3.3.13 E. carotovora ssp. atroseptica XI TG105(a) R3 P.infestans VII CP56 Gro 1, G. rostochiensis XII CP60 (CAPs) Rx1, PVX, P. infestans VII gro1-11p41 Gro 1, G. rostochiensis XII GP122 PVY gro1-2p40 (ASO) Rx1, PVX, P. infestans, GPa2, VII Gro 1, G. rostochiensis XII GP34 (ASO) G.pallida VII Gro1-4 Gro1, G.pallida X TG63 Rber, P. infestans VII GP219 E. carotovora ssp. atroseptica XII PR-5 PRP VIII RC5t7 Osm8, PRP V CT51 (CAPS) H1, G. rostochiensis VIII SC811 Ns, PVS V 239E4left (CAPS) H1, G. rostochiensis VIII Osm P.infestans VIII Rpi-blb1 , P.infestans VIII CP53 Rblc P.infestans VI Rpi-blb2 , P.infestans IV Th21 (SCAR) Rpi-blb3 , P.infestans
  • 18. Figura 1. Amplificación de fragmentos en especies silvestres de papa de Costa Rica con imprimadores específicos para genes ligadoscon resistencia a G. rostochiensis en papa cultivada. M= 100 bp DNA Ladder Plus (Fermentas), C= variedad de papa control P6-210,17-62 = diferentes accesiones de papa silvestre de Costa Rica.
  • 19. Marcador 239E4left. M: Marcador de peso molecular 100pb. Muestras 1-16 accesiones de papa silvestre, 1: 146, 2:160 3:134, 4: 147, 5: 130, 6:157, 7:121, 8:127, 9:135, 10: 148, 11:118, 12:150, 13:151, 14:152.
  • 20. Marcador GP179. M: Marcador de peso molecular 100pb. Muestras 1-16 accesiones de papa silvestre, 1: 146, 2:160 3: 134, 4:147, 5: 130, 6:157, 7:121, 8:127, 9:135, 10: 148, 11:118, 12:150, 13:151, 14:152.El marcador GP179 (550pb) se ha relacionado con el QTL GpaV (de resistencia a G. pallida) y con el QTL Grp1 de amplioespectro para G. pallida y G. rostochiensis (Meksem et al.1995).
  • 21. Thanks for your attention