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Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
Core assay list 2011
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Core assay list 2011

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Natural Remedies offers in vitro assay services

Natural Remedies offers in vitro assay services

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  • 1. In Vitro Cell Based assay List
  • 2. CORE ASSAY LIST 2011THERAPEUTIC AREA – IMMUNOMODULATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of mitogens/inhibitors on cell proliferation in murine splenocytes/Human PBMC. Useful for lead Up to 3 compounds at CellCA01 optimization of proliferation/ anti-proliferation compounds. 4 Weeks 10 mg 5 concentrations tested Proliferation Assay format: Incubations in 48-well plates. MTT read out. in quadruplicate Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (un-stimulated) Nitric Oxide release from murine macrophages. Useful for lead Up to 4 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA02 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. Colorimetric. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-2) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA03 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-3) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA04 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 3. THERAPEUTIC AREA – IMMUNOMODULATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) cytokine release (IL-4) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA05 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-5) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA06 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-6) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA07 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-10) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA08 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 4. THERAPEUTIC AREA – IMMUNOMODULATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) cytokine release (IL-12) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA09 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IFN-γ) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA10 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 5. THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) Nitric Oxide release from murine macrophages. Useful for lead Up to 4 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA11 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. Colorimetric. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Prostaglandin release (PGE2) from murine macrophages. Useful Up to 4 compounds at Mediator for lead optimization for Anti-inflammatory compounds andCA12 4 Weeks 10 mg 5 concentrations tested release potency determination. in triplicate Assay format: Incubations in 96-well plates. HTRF. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Thromboxane release (TXB2) from Human pro-myelocytic Up to 4 compounds at Mediator leukemia cells. Useful for lead optimization for Anti-inflammatoryCA13 4 Weeks 10 mg 5 concentrations tested release compounds and potency determination. in triplicate Assay format: Incubations in 96-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Leukotriene release (LTB4) from Human pro-myelocytic leukemia Up to 4 compounds at Mediator cells. Useful for lead optimization for Anti-inflammatoryCA14 4 Weeks 10 mg 5 concentrations tested release compounds and potency determination. in triplicate Assay format: Incubations in 96-well plates. HTRF. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 6. THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) cytokine release (IL-1β) from murine macrophages. Useful for lead Up to 4 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA15 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Histamine release from rat basophilic leukemia cells. Useful for Up to 4 compounds at Mediator lead optimization for Anti-allergic compounds and potencyCA16 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. HTRF. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (MCP-1) from murine peritoneal macrophages. Up to 4 compounds at Mediator Useful for lead optimization for Anti-inflammatory compounds andCA17 4 Weeks 10 mg 5 concentrations tested release potency determination. in triplicate Assay format: Incubations in 96-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 7. THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Measurement of compound toxicity in hepatocytes against t-BH using viability (MTT) endpoints. Assess compound Up to 4 compounds at In vitro safety before proceeding to clinical development.CA18 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file. Test model: Measurement of compound toxicity in hepatocytes against diazinon (OPC) using viability (MTT) endpoints. Assess Up to 4 compounds at In vitro compound safety before proceeding to clinical development.CA19 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file. Test model: Measurement of compound toxicity in hepatocytes against Aldrin (OC) using viability (MTT) endpoints. Assess Up to 4 compounds at In vitro compound safety before proceeding to clinical development.CA19 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file. Test model: Measurement of compound toxicity in hepatocytes against Aflatoxin (AFB1) using viability (MTT) endpoints. Assess Up to 4 compounds at In vitroCA20 compound safety before proceeding to clinical development. 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 8. Data format: Word™ report with data available in an Excel™ file.THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Measurement of compound toxicity in hepatocytes against Galactosamine using viability (MTT) endpoints. Assess Up to 4 compounds at In vitro compound safety before proceeding to clinical development.CA21 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file.THERAPEUTIC AREA – COSMETOLOGY Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (un-stimulated) collagen release from primary human dermal fibroblast. Useful for Up to 4 compounds atCA22 Anti-wrinkle lead optimization for cosmetology ingredient. 4 Weeks 10 mg 5 concentrations tested Assay format: Incubations in 96-well plates. Colorimetric. in triplicate Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (un-stimulated) Melanin release from mouse melanoma cells. Useful for lead Up to 4 compounds at SkinCA23 optimization for cosmetology ingredient. 4 Weeks 10 mg 5 concentrations tested whitening Assay format: Incubations in 96-well plates. Colorimetric. in triplicate Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 9. THERAPEUTIC AREA – DIABETES/OBESITY Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model: Measurement of induction of glucose stimulated Insulin secretion in rat insulinoma cells. This approach is useful for assessing the effectiveness of compounds under development for Up to 4 compounds atCA24 GSIS Assay the treatment of type 1 diabetes. 4 Weeks 10 mg 5 concentrations tested Assay Format: Incubations in 96-well plates. HTRF. in triplicate Data Format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Lipid Adipocyte differentiation in Mouse embryonic fibroblast . Useful Up to 4 compounds at MetabolismCA25 for lead optimization for inhibitors of adipogenesis. 4 Weeks 10 mg 5 concentrations tested Assays Assay format: Incubations in 96-well plates. Colorimetric. in triplicate (Adipogenesis) Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Lipid glycerol release in Mouse embryonic fibroblast . Useful for loead Up to 4 compounds at MetabolismCA26 identification for inhibitors of adipolysis. 4 Weeks 10 mg 5 concentrations tested Assays Assay format: Incubations in 96-well plates. Colorimetric. in triplicate (Adipolysis) Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 10. THERAPEUTIC AREA – ONCOLOGY Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model: Determine effect of compounds on cell proliferation in human breast adenocarcinoma cells. Useful for lead optimization Anti Up to 4 compounds at of anti-cancer compound screening.CA27 proliferative 4 Weeks 10 mg 5 concentrations tested assay Assay Format: Incubations in 96-well plates. Colorimetric. in triplicate Data Format: WordTM report with data available in an ExcelTM file.THERAPEUTIC AREA – GENERAL HEALTH/ HEART DISEASE Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model: Determine the cellular antioxidant potential of compounds in Human hepatocellular liver carcinoma cells. Useful cost-effective method to evaluate the antioxidant activity of foods, Up to 4 compounds at AntioxidantCA28 dietary supplements, phytochemicals and extracts.. 4 Weeks 10 mg 5 concentrations tested assay Assay Format: Incubations in 96-well plates. Colorimetric. in triplicate Data Format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  • 11. THERAPEUTIC AREA – IN VITRO GENOTOXICITY Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model:. Determine the mutageneic potential of compounds using 5 starins of S.Typhimurium with and without S9 fractions. Up to 1 compounds at Mutagenesis Quick assay to estimate the carcinogenic potential of a compoundCA29 4 Weeks 10 mg 6 concentrations tested assay Assay Format: Incubations in 384-well plates. Hightroughput. in duplicate. Data Format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on the formation of small membrane bound DNA fragments i.e. micronuclei in the cytoplasm of interphase cells using Chinese hamster cells. Useful Up to 1 compounds at MutagenesisCA30 for early identification of compounds likely to exhibit genotoxic 15 Weeks 10 mg 3 concentrations tested assay liability. in duplicate. Assay format: Incubations in 90mm petri plates. Manual. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on using induction of structural changes in chromosomes Chinese hamster cells. Useful Up to 1 compounds at Cytogenetic to identify agents that cause structural chromosome aberration inCA31 15 Weeks 10 mg 3 concentrations tested Assays cultured mammalian cells. in duplicate. Assay format: Incubations in 90mm petri plates. Manual. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com

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