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PPE & Safety in Lab - Prac. Microbiology
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PPE & Safety in Lab - Prac. Microbiology



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  • 2. Personal Protective Equipment (PPE) “Specialized clothing or equipment worn by an employee for protection against infectious materials”
  • 3. How Can I Select PPE? PPE selected for use should: Protect HCP clothing and skin from contamination by blood and body fluids.  Protect patients from acquiring microorganisms from HCP.  ICC HEEPF ICEP
  • 4. What are the types of PPE? Gloves  Gowns and aprons  Mask  Eye goggles  Overhead  Foot protection ??? 
  • 5. Types of Gloves Sterile Gloves  Non-sterile Gloves.  Heavy-duty Gloves. 
  • 6. Sterile Gloves   Disposable, individually wrapped. They are mostly used during surgery and during performing invasive procedures.
  • 7. Non- Sterile Gloves   Disposable, single use, usually not singly wrapped. They are mostly used to protect skin against exposure to blood and body fluids (e.g. when changing dressing and handling specimens).
  • 8. Heavy – Duty Gloves reusable after decontamination Used for:  Handling medical waste  Performing environmental cleaning
  • 9. Aprons and Gowns Used during procedures that generate splashes of blood or body fluids. Apron Gown
  • 10. Eye Goggles Used for protection against splashes of blood or body fluids (eg. during certain surgical procedures). 
  • 11. Overhead Used to contain hair during surgical procedures
  • 12. Masks Placed over nose, mouth and chin Standard mask Used when there is risk of exposure to droplets containing infectious agents N95 masks Used when there is risk of exposure to airborne infectious agents such as M. tuberculosis (prevent >95% of particles less
  • 13. Closed Foot Wear In operating theatre Over-shoes “NOT RECOMMENDED” Ideal way of transferring micro-organisms from floor and shoes to the hand.
  • 14. What’s WRONG ?????
  • 15. Safety in the laboratory
  • 16. The laboratory safety procedures must be read prior to attending the laboratory session.
  • 17. WHY?? Important in prevention of:  Infection  Injury by harmful chemicals  Mechanical injury by glassware, open flames, and sharp objects.
  • 18.  Personal belongings away from workstation  - Wash your hands with soap at the start of the laboratory session before performing any procedures before leaving the laboratory at the end of the session.
  • 19. Personal Protective Equipment (PPE) used: o Gloves are essential. o Lab coats are required. o Safety glasses ( goggles) may be required to avoid splashes.
  • 20. Avoid touching objects (e.g., pencils, cell phones, door handles) while wearing gloves.
  • 21. Long hair must be tied back or covered to minimize fire hazard or contamination of experiments.
  • 22. Laboratory personnel should not wear sandals
  • 23. Smoking in the lab is essentially prohibited
  • 24. Eating and drinking in the laboratory are absolutely prohibited.
  • 25.    All cultures should be handled as potentially pathogenic. Liquid cultures must always be kept in a test tube rack. Broth cultures must never be pipetted by mouth.
  • 26. In case of Blood Spills      Cover with paper towel. Apply disinfectant. Leave for 15 minutes. Remove while wearing gloves. Discard in a red bag.
  • 27. Report all accidents, no matter how minor, to your supervisor!!