Revision Scheme - Prac. Microbiology

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Revision Scheme - Prac. Microbiology

  1. 1. SLIDES
  2. 2. Schemes for slides With background Without background Gram positive cocci Gram positive bacilli Gram negative cocci
  3. 3. Scheme for slides With background Without background Brown Blue Red Organism Gram +ve cocci Organism Gram -ve
  4. 4. Staphylococci in culture
  5. 5. Staphylococci in culture
  6. 6. Streptococci in culture
  7. 7. Streptococci in culture
  8. 8. Candida
  9. 9. Neisseria in culture
  10. 10. Corynebacterium diphtheriae
  11. 11. Clostridium tetani
  12. 12. Clostridium tetani
  13. 13. Staphylococci in pus
  14. 14. Staphylococci in Pus
  15. 15. Streptococci in Pus
  16. 16. Streptococci in pus
  17. 17. Vincent’s angina
  18. 18. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  19. 19. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  20. 20. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  21. 21. Spirochaetes (Fontana stain)
  22. 22. Spirochaetes (fontana stain)
  23. 23. Spirochaetes (fontana stain)
  24. 24. MEDIA
  25. 25. Nutrient agar
  26. 26. Staphylococcus aureus on nutrient agar ( showing golden yellow endopigment )
  27. 27. Blood agar
  28. 28. Types of hemolysis on Blood agar
  29. 29. Alpha-hemolysis on blood agar
  30. 30. Beta-hemolysis on blood agar
  31. 31. Non-hemolysis on blood agar
  32. 32. Chocolate agar for Neisseria
  33. 33. Loffler’s serum for C. diphtheriae
  34. 34. Lowenstein Jensen medium for M. tuberculosis
  35. 35. Cooked meat medium for anaerobic cultivation
  36. 36. TESTS
  37. 37. Coagulase Test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
  38. 38. Coagulase test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
  39. 39. Antibiotic Sensitivity Test (Disc diffusion) Sensitive to: 1, 2, 3, 4, 6 Resistant to: 5 1 5 62 4 3
  40. 40. Antibiotic Sensitivity Test (Disc diffusion) Sensitive to: 1, 2, 5, 6 Resistant to: 3, 4 1 5 6 2 4 3
  41. 41. Phage typing 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Sensitive to 4, 5, 6,……..etc.
  42. 42. SEROLOGY
  43. 43. Elek’s Test Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae
  44. 44. Simple tube agglutination test (Titre:1/320) TITRE 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control

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