Clostridium - Prac. Microbiology

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Clostridium - Prac. Microbiology

  1. 1. Members of genus Clostridium are relatively large, anaerobic, Gram-positive, spore forming rods Many clostridia are members of the normal flora of the gastrointestinal tracts of humans and animals
  2. 2. Morphology
  3. 3. Thioglycolate broth
  4. 4. Clostridium tetani
  5. 5. Morphology • Gram positive bacilli • Spores (terminal & bulging) Drumstick appearance • Non capsulated • Motile Culture • Strict anaerobe • Complete haemolysis
  6. 6. C. tetani produces two toxins: 1.Tetanolysin 2.Tetanospasmin A neurotoxin responsible for the symptoms of tetanus and is encoded on a plasmid .
  7. 7. Pathogenesis
  8. 8. Treatment: Human Tetanus Immunoglobulin (HTIG) given IM (human better than horse) - Wound debridement - Metronidazole Prevention: DPT Vaccine 2, 4 & 6 months & booster doses
  9. 9. 1) C. tetani has the following morphology: a)Gram negative cocci arranged in pairs b)Gram positive cocci arranged in chains c)Gram positive club-shaped bacilli d)Gram positive bacilli with terminal bulging spores e)Gram positive capsulated diplococci
  10. 10. 2) All of the following are methods of anaerobic culture EXCEPT: a)Robertson cocked meat media b)Anaerobic jar c)Thioglycolate broth d) Deep agar e)Blood agar
  11. 11. 3) Clostridium tetani causes: a) Gas gangrene b) Tetanus c) Botulinum food poisoning d) Antibiotic associated diahrrea e) Scarlet fever
  12. 12. 4) Clostridium tetani: a) Causes flaccid paralysis b) Is an aerobic bacillus c) Has two toxins: tetanolysin and tetanospasmin d) Produces an enterotoxin as the virulence factor e) Is treated by a toxoid

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