BIOL 4997: BIOMEDICAL TECHNIQUESREFLECTIONScience is a huge world where the countries can be called laboratories and the population,research. Each country has unique characteristics that defined their populations. Well, just likecountries, each research has specific techniques that are determined by the main goal. TheBiomedical Techniques course prepares the student to confront any situation of research by usingthe right technique.By experiencing different workshops these techniques are well learned and develop.Someof the workshops offered were: Importance of pipetting and practice,Microscopy andphotomicrography,From DNA to protein, Protein interactions an Insilico approach, Magneticnanoparticles based: separation of proteins, Phages and proteomics and Water sampling, testing,and statistical application. Of all the workshops the ones that I liked the most were: Microscopyand photomicrography, Protein interactions an Insilico approach, and Water sampling, testing,and statistical application.Since last summer, when I used a microscope for the first time, I have been amazed withmicroscopes. Dr. Ross thought us how to use different microscopes. Some of these were thedissecting microscope and the fluorescence microscope. The dissecting microscope lets you domore specific cuts and a clearer view of your dissection. During the workshop we experiencedwith different types of insects how the specimen really looked like. I enjoyed the assignment alot. Nicole taught me how to use the fluoresce microscope and I was amazed by the waychloroplasts looked like. I spent hours and hours selecting different effects and taking pictures ofchloroplasts and insects. But at the end the most important lesson was that microscopes areessential to understand Biology and different approaches.Dr. Maldonado’s workshop was really special to me, because the techniques learned thatday helped me to develop my final project which was: In-Silico Discovery of Influenza virusPolymerase PB1-PB2 Protein Complex Inhibitors. In-Silico programs are a new and innovativeway to discover new drugs that have the potential to become effective treatments to illness anddiseases. The best characteristic that this technique has is that is really fast and shows theinhibition and interaction between proteins in a 3D way.Water is the most important component of the world. Testing its quality in an island thatyear after year is more contaminated and more populated, makes the workshop provided by Dr.Arce one that should be thought to all Puerto Ricans. Using a quick technique the total coliforms
and fecal coliforms were counted. These provided the essential information needed to determinethe quality of water.At the end of the course I learned a lot of techniques that will help me in futureresearches or laboratory classes. The opportunity to perform two research projects, obtain resultsand shared them was really rewarding. Not to mention, that being part by collaborating in thePhage Hunting Program, was really exciting for this scientists in progress. Another experiencethat I would like to share a reflection was the different workshops that were provided by twoinstitutions from the United States. Thanks to the University of North Carolina and the MichiganState University to taught to us about DNA and the Nervous System, respectively. The overallexperience was amazing and it forms know another reason to obtain a Ph.D.