Biological Microscopes Macro Imaging Confocal Imaging 2009-10 Live-cell Confocal Microscopy Patch Clamping Image data courtesy of: Pr. Kentaro Arikawa, Ms. Mituyo Kinoshita, Laboratory of Neuroethology,Graduate School of Integrated Science, Yokohama City University (cover page left top) Atsushi Miyawaki M.D., Ph.D, Ms. Ryoko Ando, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics (cover page above middle) Mark Ellisman Ph.D, Hiroyuki Hakozaki M.S., Natalie Maclean M.S., University of California, San Diego, NCMIR (back page left top) Tohru Murakami M.D., Ph.D, Department of Neuromuscular and Developmental Anatomy, Gunma University Graduate School of Medicine (back page left bottom)Products with Olympus Eco-products mark in this catalog are environmentally conscious • OLYMPUS CORPORATION has obtained the ISO9001/ISO14001products made according to OLYMPUS standard. • OLYMPUS CORPORATION has obtained the MD540624/ISO13485This does not apply to some accessories. • Illumination devices for microscope have suggested lifetimes.Please visit our website for more information: http://www.olympus.co.jp/en/eco-products Periodic inspections are required. Please visit our web site for details. • Images on the PC monitors are simulated. • Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer. Printed in Japan M05E-0909B
N ew avenues of research are opening in biological and medical fields. As research demands become more specialized anddiversified, biological microscopes must offer the capabilities to Contents Fluoview FV1000MPE Multiphoton Laser Scanning Biological Microscope ... 3 Fluoview FV1000 Confocal Laser Scanning Biological Microscope ... 3meet these needs. Fluoview FV10i Confocal Laser Scanning Biological Microscope ... 4Olympus microscopes and their accessories are developed to meet IX2-DSU/BX-DSU Biological Disk Scanning Microscope ... 4the ever-changing needs of research applications. FSX100 Biol Imaging Navigator ... 5Our accomplishments in microscope development date back more BX61 Motorized System Microscope ... 5than three-quarters of a century. Olympus has accumulated a broad BX51 System Microscope ... 6 BX41 Laboratory Microscope ... 6range of advanced optical and precision technologies and we are BX45 Ergonomic Laboratory Microscope ... 7renowned for our innovative approach to microscopy. BX51WI Fixed Stage Upright Microscope/ BX61WI Fixed Stage Upright Microscope with Motorized Focusing ... 7An outstanding example of Olympus ingenuity is the superior UIS2 Accessories for BX2 ... 8infinity-corrected optical system employed on the BX2 and IX2 CX41 System Microscope/CX31 Biological Microscope ... 9microscopes. Olympus has also won acclaim for its system CX21LED/CX21 Biological Microscopes ... 9versatility and broad range of advanced accessories. IX81 Motorized Inverted System Microscope ... 10 IX71 Research Inverted System Microscope/Our microscopes are evolving with enhanced performance and IX51 Inverted System Microscope ... 10 Accessories for IX2 ... 11operational ease. Olympus continues to answer research demands in ON3 Series Micromanipulators ... 12the biological and medical field of today and pave the way for future CKX41/CKX31 Inverted Microscopes ... 13advances with increasingly sophisticated research equipment. Accessories for CKX41/CKX31 ... 13 BX51-P Polarizing Microscope ... 14 CX31-P Polarizing Microscope ... 14 SZX16/SZX10 Research Stereomicroscope System ... 15 SZX7 Stereomicroscope System ... 16 SZ61/SZ51 Stereomicroscope System ... 16 Accessories for SZX/SZ ... 17 Macro View MVX10 Research macro zoom system microscope ... 18 DP72 Digital Camera ... 19 DP25 Digital Camera ... 19 DP20 Digital Camera ... 19 E330M1.2x E-330 Micro Imaging System ... 19 UIS2 Series Objectives ... 20-22 UIS Series Objectives ... 20, 22
The FV1000MPE allows outstanding fluorescence imaging down into deep Fluoview FV10i is the worlds first self-contained Confocal Laser FLUOVIEW FV1000MPE regions of the specimens. Using pulsed IR (infrared) lasers in combination with our long working distance objectives, the FV1000MPE is able to image hundreds of microns into living tissues, providing the highest penetration depths on the FLUOVIEW FV10i Scanning Microscope. The FV10i has the same functionality of a high end confocal laser scanning microscope with easy-to-use software delivering it in a compact design. MULTIPHOTON LASER SCANNING CONFOCAL LASER SCANNING market. Beside the ability to penetrate deep into a specimen, long wavelength IR •The system is equipped with 4 wavelength diode lasers BIOLOGICAL MICROSCOPE light has another advantageous property; its lower energy content reduces BIOLOGICAL MICROSCOPE The system is equipped with four lasers. Multi-stained specimens can be imaged with up phototoxicity and the risk of photodamage thus making long range and time- to four fluorescence dyes. Maintenance-free and power-saving diode laser with longer lapse studies possible. The FV1000MPE provides cutting-edge technology for operating lives are employed in all the laser units, and operate with low noise levels. various areas of scientific research such as neuroscience and cell biology and is •Detector utilizes a newly developed spectrum method available in three configurations. The detecting mechanism has two fluorescence channels, and one phase contrast *FV1000MPE is a class 4 laser product. channel. The fluorescent channels use a newly developed spectrum method comprising grating, beam splitter, and slit. Laser IR pulsed laser Mode-locked Ti:sapphire laser [femtosecond laser (equipped with In addition, they are equipped with the variable barrier filter function where the most unit with negative chirp a group velocity dispersion correction/control device)], suitable wavelength width is set automatically in accordance with the characteristics of laser power unit, water-cooled circulating chiller the fluorescence dye. Visible light laser LD laser: 405 nm (50 mW), 473 nm (15 mW), 559 nm (15 mW), *FV10i is a class 1 laser product. AOTF laser 635 nm (20 mW) combiner *1 Multi Ar laser (458 nm, 488 nm, 515 nm, Total 30 mW) HeNe (G) laser (543 nm, 1 mW) Laser unit Ultraviolet / Visible light 405 nm (22 mW), 473 nm (15 mW), 559 nm (18 mW), Visible light laser platform with implemented AOTF system LD lasers 635 nm (12 mW) Single laser for LD473 laser (15 mW) Modulation Continuously Variable by the LD direct modulation visible light *1 (0.1% – 100%, 0.1% inclement) Scanning Confocal detector 3 channels for fluorescence detection (photomultipliers), Line return period - laser OFF unit module *1 optional 4CH detector for expansion Scanning Confocal detector Fluorescence: 2 channels, Phase Contrast: 1 channel Scanning method 2 galvanometer scanning mirrors unit module Variable barrier filter mechanism for fluorescence channel by Scanning modes Pixel size: 64 x 64 – 4096 x 4096 pixels diffraction grating and slit Scanning speed: (pixel time): 2 µs – 200 µs Scanning method 2 galvanometer scanning mirrors Dimensions: Time, Z, (wavelength) (or any combination thereof) Scanning modes Pixel size: 256 x 256 – 1024 x 1024 Line scanning: straight line, free line, point XY scan Scanning speed: 1.1 s / frame Field Number 18 (for pixel size 512 x 512, High Speed scanning mode) Optical Zoom 1X – 50X in 0.1X increment Dimension: XYT, XYZ, XYZT Rotation scanning: 0 – 359.9º in 0.1º increments Detector Reflected light Photomultiplier (2 or 4 channels),Fluorescence wavelength can be for multi- fluorescent detector selected with the dedicated filter cube (replaceable) Field Number 18 photon Transmitted light Photomultiplier (2 channels), Optical Zoom 10x objectives: 1x – 6x (adjustable in 0.1X increments) imaging fluorescent detector Exclusive equipment for the BX61WI upright microscope 60x objectives: 1x – 10x (adjustable in 0.1X increments) FV1000MPE basic system, BX61WI configuration Z-drive Motorized focus Microscope Inverted IX81, Upright BX61, Upright focussing nosepiece & fixed stage BX61WI Minimum increment: 0.01 µm Z-drive A motorized focus module inside the microscope is used *Please refer to FV10i catalog for further details Minimum increment: 0.01 µm *1: The M scanner does not have an optional confocal detector *Please refer to FV1000MPE catalog for further details3 4 The FV1000 has an original spectral detection system which uses a The DSU (Disk Scanning Unit) is compatible with both inverted and FLUOVIEW FV1000 high speed diffraction grating combined with a variable slit to deliver superior linear spectral distribution. This enables high-precision, high- resolution, high-speed spectroscopy in observations ranging from IX2-DSU/BX-DSU upright microscopes, including the motorized IX81 and BX61 making it an ideal imaging system for automated Z-stack, 3-D image analysis. CONFOCAL LASER SCANNING BIOLOGICAL DISK SCANNING milliseconds to hours. SIM (SIMultaneous) Scanner System BIOLOGICAL MICROSCOPE synchronizes laser light stimulation and confocal imaging. The FV1000 MICROSCOPE •The DSU system also supports DAPI excitation in the near UV without modification. Filter-mirror sets for GFP and DsRED are included. incorporates 2 laser scanners for simultaneous observation and laser •The DSU is excellent for live-cell applications where speed of acquisition light stimulation. The FV1000 is the most suitable choice of and minimal phototoxicity is paramount. microscope for FRAP, FLIP and photo activation. •Five disks are available of varying slit width and spacing allowing the user to optimize disk performance to different objective numerical apertures *FV1000 is a class 3B laser product. and specimen thicknesses. Spectral type fluorescence detector Filter type fluorescence detector Confocal scan method Disk rotation method Laser Ultraviolet/ LD lasers: 405 nm (50 mW), 473 nm (15 mW), 559 nm (15 mW), unit Visible light 635 nm (20 mW) Maximum scan speed Image acquisition less than 33 msec/frame laser Multi-line Ar laser (458 nm, 488 nm, 515 nm, Total 30 mW), Excitation wavelength 350 nm -700 nm. HeNe(G) laser (543 nm, 1 mW) Wavelengths of less than 430 nm at naked eye AOTF laser Visible light laser platform with implemented AOTF system observation and DSU observation may reduce the combiner confocal effects. Fiber Broadband type (400 nm–650 nm) Fluorescence wavelength At less than 450 nm, use our HQ filter for observation at observation Scan- Detector Standard 3 confocal Channels. Standard 3 confocal Channels. ning module Spectral detection : CH1 to CH3 each with 6 position Observation mode Exchange between confocal and non-confocal modes unit CH1 and CH2 equipped with barrier filter turret. can be performed through the software independent grating. ND filter for excitation An ND filter will be inserted automatically at the exchange CH3 with 6 position barrier filter turret of confocal and non-confocal modes Scanning method Dual galvano mirror scanner (X, Y) Electromagnetic shutter Can be controlled through the software Scanning pixel size:64 x 64 – 4096 x 4096 for excitation modes Pixel Dwell time: 2 to 200 microsec with unidirectional Microscope attachment Intermediate attachment method (other cameras can be 0.5 or 1 microsec with fast bidirectional scanning mounted on C-mount intermediate attachment) X,Y,T,Z,λ (any combination) *Please refer to IX2-DSU/BX-DSU catalog for further details Line scanning: Straight line with free orientation, free line Field Number (N.A.) 18 Optical Zoom 1X – 50X in 0.1X increment Z-drive Motorized focus module of the microscope, minimum increment 10 nm Transmitted External transmitted photomultiplier detector light detector unit Micro- Motorized Inverted IX81, Upright BX61, scope microscope Upright focussing nosepiece & fixed stage BX61WI FV1000+IX81 Option- SIM 2 Galvano scanning mirrors, pupil projection lens, al Scanner built-in laser shutter, 1 laser port unit Fiber introduction of near UV laser diode or visible light laser, IX2-DSU Optional: 2nd AOTF laser combiner *Please refer to FV1000 catalog for further details
Crisp microscope images, consistently captured using only This leading model from the BX2 series offers improved ergonomic FSX100 a computer mouse. FSX100 make three major innovation "Super-easy operation", "Superb image quality" and "Labor-saving work flow" and system performance, and is widely used in both routine work and specialized research. It is equipped with Olympus’ original UIS2 optic system, and a high-rigidity Y-shape frame with newly BIO IMAGING NAVIGATOR SYSTEM MICROSCOPE refined ergonomics. Excellent features provide the flexibility •The FSX100 is a fully motorized system. Specimen movement and all needed for compliance with a variety of applications. In addition, microscope imaging modes are motorized and operated via a host computer/mouse. many kinds of filter sliders and accessories are all designed with •Centered around our world leading 40x dry objective with an multiple openings on the bodies and illuminators. unprecedented numerical aperture 0.95, the FSX100 captures high resolution and contrast fluorescence images of even weakly labeled •Can be combined with a swing-out condenser and a septuple revolving specimens. nosepiece with DIC slider to enable continuous observations from 1.25X •Incorporating a unique external shape and a proprietary internal optical to 100X. design, the FSX100 is designed to be incorporated into your lab space •A newly developed 8-position universal condenser gives even greater easily. freedom to combine observation methods. Observation mode Fluorescence/Phase contrast, Phase contrast, and Bright field Illumination Transmitted light 12V100W halogen Koehler illumination Acquisition mode Single, Time Lapse, Z-stack, and Stitching Focusing Coarse & fine coaxial handle: (fluorescence multi color imaging possible for all) full stroke: 25mm, minimum fine adjustment: 1µm Automatic focus (AF) Automatic during the screen transition with one click Observation tube Widefield binocular (F.N.22), widefield tilting binocular (F.N.22), operation widefield trinocular (F.N.22), widefield tilting/telescoping binocular (F.N.22), super widefield trinocular (F.N.26.5) Standard objectives Capture: 40x, NA 0.95 (17x to 80x with optical zoom) Macro: 10x, NA 0.40 (4.2x fixed with optical zoom) Nosepiece Interchangeable reversed quintuple/sextuple/septuple Motorized correction collar with focusing assist Stage Ceramic-coated coaxial with left or right hand low drive control, non-stick grooved coaxial, plain, rotatable Fluorescence filter U excitation (BP 360-370, BA 420-460, DM400) B excitation (BP 460-495, BA 510-550, DM505) Condenser Abbe (N.A.1.1), swing out Achromatic (N.A.0.9), G excitation (BP 530-550, BA 575IF) Achromatic Aplanatic (N.A.1.4), Universal (N.A.1.4/0.9) Camera type Single-panel color CCD pixel shift type Other features Coarse/fine changeover button, stage shunting button, stage up/down button, built-in filters (LBD-IF, ND6, ND25, option) Effective image resolution 4080 x 3072 (12.5 megapixels), 2040 x 1536 (3.1 megapixels), Accessories Reflected light fluorescence attachment, DIC attachment, 1360 x 1024 (1.4 megapixels), phase-contrast attachment, multi-viewing attachment, 680 x 512 (350,000 pixels), polarizing attachment, etc. 2 x 2 binning; 680 X 510 (350,000 pixels), *Please refer to BX51/BX61 catalog for further details 4 x 4 binning; 340 x 250 (85,000 pixels) *Please refer to FSX100 catalog for further details5 6 Motorized system microscope BX61 in the BX2 series features This key model in the BX2 series offers excellent cost-performance outstanding reliability in highly advanced microscopy such as three and meets a wide variety of needs in the routine inspection of dimensional imaging capture with high-end fluorescence imaging laboratories and clinics. The extensive range of features benefits MOTORIZED SYSTEM MICROSCOPE and confocal microscope. from consistently simple operation, enhanced by Olympus’ original Standard features include motorized focusing and light adjustment LABORATORY MICROSCOPE and ergonomically-advanced Y-shape frame design and UIS2 and a stage escape mechanism. Several key macro microscope optical system. This model allows easy system expansion: by operations are available by software-controlled setting, and combining multiple units together, it can meet the majority of executed by hand switch, personal computer. general working requirements. •Uses flexible software which can easily be customized for specific •Newly developed 30W halogen light source provides as much brightness purposes. as a conventional 50W halogen lamp. •Many operating procedures (including switching between observation •Equipped with an easy-to-operate rackless stage with no protrusion of methods) are stored as individual macros and allotted to buttons on the the X-axis guide. microscope, hand switches and keys on the monitor of the personal •The front-located power switch and light intensity volume control make it computer. possible to operate the microscope with the arms resting on the desk. •A wide variety of separate modules make expansion very easy. •A 3-filter cassette enables quick, easy exchange. •The detachable revolving nosepiece allows quick exchange of objectives to suit different observation methods. Illumination Transmitted light 12V100W halogen Koehler illumination Focusing Motorized focus Full stroke: 14mm, minimum fine adjustment: 0.01µm Illumination Transmitted light 6V30W halogen Koehler illumination Observation tube Widefield binocular (F.N.22), widefield tilting binocular (F.N.22), Focusing Vertical stage movement: 25mm; Stage stroke with coarse widefield trinocular (F.N.22), widefield tilting/telescoping binocular adjustment limit stop; Torque adjustment for coarse adjustment (F.N.22), super widefield trinocular (F.N.26.5) knobs; Stage mounting position variable; High sensitivity fine Nosepiece Interchangeable reversed quintuple/sextuple/septuple, motorized focusing knob (adjustment graduations 1µm) sextuple with slider slot for DIC, septuple for DIC/simple POL Observation tube Widefield binocular (F.N.22), widefield tilting binocular (F.N.22), Stage Ceramic-coated coaxial with left or right hand low drive control, widefield trinocular (F.N.22), widefield ergo binocular (F.N.22) non-stick grooved coaxial, plain, rotatable super widefield trinocular (F.N.26.5) Condenser Abbe (N.A.1.1), swing out Achromatic (N.A.0.9), Nosepiece Interchangeable reversed sextuple/quintuple Achromatic Aplanatic (N.A.1.4), Universal (N.A.1.4/0.9) Stage Ceramic coated coaxial with right or left hand low drive control Other features Coarse/fine changeover button, stage shunting button, stage and rotating mechanism, non stick grooved coaxial, plain, up/down button, built-in filters (LBD-IF, ND6, ND25, option) rotatable Accessories Motorized fluorescence illuminator, motorized universal Condenser Abbe(N.A.1.1), swing-out Achromat (N.A.0.9), condenser, motorized transmitted filter wheel, motorized Achromatic Aplanat (N.A.1.4), phase-contrast/darkfield (N.A.1.1), reflected filter wheel, motorized observation filter wheel, darkfield dry (N.A,0.8-0.92), darkfield oil (N.A.1,20-1,40), hand switch, control unit, etc. ultra low (N.A.0.16) Other feature Light pre-set switch *Please refer to BX51/BX61 catalog for further details Accessories Reflected light fluorescence attachment, DIC attachment, phase-contrast attachment, multi-viewing attachment, etc. *Please refer to BX41/BX45/BX51 catalog for further details
Accessories for BX2 This high-performance laboratory microscope is equipped with UIS2 optics that provide excellent image quality, and the same ergonomically advanced Y-shape frame as other models in the BX2 series. Incorporated in a 3-position universal condenser which ERGONOMIC LABORATORY MICROSCOPE complies with brightfield, phase-contrast and darkfield observations, and a gout inspection analyzer that uses simple polarizing observation. Enables optimum illumination for observations from 1.25X to 40X. •Use no-cover objectives which are suitable for observing smear specimens (e.g. blood) with no cover glass. •2x,4x,10x and 20x objectives equipped with ND filter enable the same BX-RFAA U-FWR, U-FWO, U-FWT U-UCD8A-2 level of brightness even if the magnification is changed from 2x to 40x. No brightness adjustment (e.g.of light intensity) is required. Motorized fluorescence illuminator Filter wheels Motorized universal condenser •Employ a rackless stage providing precise movement and smooth Up to 6 fluorescence mirror units can be Motorized exchange of 6 filters. Three Different combinations of designated stopping with no protrusion of the X-axis guide. •A convenient marking unit is provided. attached simultaneously. Mirror unit can kinds of of filters can be attached optical components allow correspondence be exchanged automatically with simultaneously: U-FWR (ø32, 25) for with various kinds of transmitted light Illumination Transmitted light 6 V 30 W halogen Koehler illumination corresponding shutter adjustment. excitation, U-FWO (ø32, 25) for absorption observations. Automatic control of optical Focusing Fixed low stage nosepiece focus and U-FWT (ø32) for transmitted light. component exchange, top lens swing Observation tube Widefield binocular (F.N.22), widefield tilting binocular (F.N.22), in/out and aperture iris diaphragm. widefield trinocular (F.N.22), widefield ergo binocular (F.N.22) Nosepiece Fixed reversed quintuple Stage Ceramic-coated coaxial with right or left hand low drive control, rotating and torque adjustment mechanism, plain, rotation Condenser Fixed 3-position universal condenser N.A.0.9 Other features One-touch marking unit, Light preset switch, Torque adjustment *Please refer to BX41/BX45/BX51 catalog for further details U-UCD8-2 U-PCD2 BX-RFA, BX-URA2 8-position universal condenser Phase/darkfield condenser BX fluorescence illuminator The universal condenser simultaneously High contrast phase imaging allows close BX reflected light illuminator accepts up to 6 DIC prisms and 2 other observation of the cell interior and of live Fully integrated into the microscope arm, optical components at maximum. bacteria. Standard brightfield and dry two illuminators add to the stability of the The condenser numerical aperture of 0.9 darkfield as well as simultaneous reflected imaging platform. BX-RFA for research7 or 1.4 (oil) can be selected through light fluorescence observations are needs and BX-URA2 for routine observation. 8 interchangeable top elements. possible. Designed for neuroscience and cell biology applications, the BX51WI offers front focus operation and a complete absence of vibration, even when switching the objectives on the nosepiece U-KPA FIXED STAGE UPRIGHT MICROSCOPE/ and filters on the turret and in the optical path of the intermediate FIXED STAGE UPRIGHT MICROSCOPE tubes. The availability of two-wavelength IR-DIC (775 nm and 900 WITH MOTORIZED FOCUSING nm) has huge advantages for researchers performing electrophysiological work using brain slice samples. Our unique two-position nosepiece can easily switch between objectives, and U-POT U-ANT prevent air bubbles forming. To avoid having to change objectives, Olympus offers a single objective (XLUMPLFLN20XW) for low to high magnifications, combined with an intermediate magnification U-RFSS U-KPO U-EXBABG, U-EXBAUB, U-EXBAUG changer. The BX51WI provides dramatically bright fluorescence Rectangular field stop Simple polarizing attachment Excitation balancer images, equivalent to our BX2 models. Designed for use with CCD cameras, Simple polarizing observation can be Used singly or in tandem, the excitation prevents photobleaching of the specimen accomplished with the combination of U- balancer curtails the individual excitation Illumination Transmitted light 12 V 100 W halogen Koehler illumination (BX51WI) outside of the imaging area. KPA intermediate attachment for simple bandwidths of the fluorochromes under Transmitted light 12 V 100 W halogen Koehler illumination; Light adjustment: less than DC2 Vg12 V (continuous adjustment) polarizing observation, U-ANT analyzer for observation. Brightness adjustment, light preset switch (BX61WI) transmitted light and U-POT polarizer. Focusing Nosepiece focus by roller guide (rack & pinion); Stroke per rotation: fine: 0.1 mm, coarse: 15 mm; Maximum stroke: 25 mm; Coarse lower limit stopper mechanism, U-SDO3, U-MDO10B3 Torque adjustment mechanism for coarse focus (BX51WI) Multi observation body Motorized focusing using stepping motor and ball screw Nosepiece focus by cross roller guide; A single image can be viewed with Minimum graduation; fine: 1 µm (sensitivity 1 µm) Resolution 0.01 µm: Maximum stage movement speed 3 mm/s the same orientation and brightness Stroke: 25 mm, stage escape mechanism (BX61WI) by up to ten persons simultaneously Observation tube Trinocular (F.N.22), erect image trinocular (F.N.22), to facilitate discussion between double port magnification change unit (F.N.22) Nosepiece Swing, Slide, Single position, Swing-slide researchers, and for training and Stage Mechanical, bridge education. No additional power unit is Condenser 8-position universal, long working distance oblique, long working distance DIC, required since the power unit of the swing-out arrow pointer is incorporated in the *Please refer to BX51WI/BX61WI catalog for further details main body. •Also available for five persons (U-MDO) and two persons in face-to-face (U-BDO). BX51+U-SDO3 BX51+U-MDO10B3
The CX41/CX31 offer extended capabilities to match a wide range This model allows researchers to customize the motorized system of applications from routine clinical work to educational use. They according to their own specific purpose, with operating control not only feature an ergonomically designed frame for maximum handled from the front. By using special software via a personal SYSTEM MICROSCOPE/ operating comfort and enhanced rigidity, but also offer the computer, it is also possible to exercise accurate control of multi- convenience of extra-bright illumination. MOTORIZED dimensional analyses, ranging from 2D to 6D. The full range of BIOLOGICAL MICROSCOPE INVERTED SYSTEM MICROSCOPE IX81performance functions, including observations, measurements CX41 ------------------------------------------------------------------------------------------------------------------------------ Featuring powerful 6V30W halogen Koehler illumination and and manipulation, can be monitored via the numerous outstanding flat images in this class of microscope, the CX41 is input/output ports, which allow connection of various kind of light applicable for a wide range of observation methods and sources and motorized modules. photomicroscopy. •Purpose-selectable motorized units and easy operation right by the CX31 ------------------------------------------------------------------------------------------------------------------------------ operators hand. An ergonomically designed frame and bright 6V30W halogen •Multi-dimensional analysis by PC control. illumination make the CX31 ideal for routine clinical work and •Maximum installation of experimental equipment and minimum layout limitations. educational applications. •Sharp, fade-free fluorescent images and faster observations. •Optimized resolution and contrast in Nomarski DIC observation, for both Illumination Built-in transmitted Koehler illuminator thick- and thin-cell specimens. 6V30W halogen bulb •Combining different light sources and video systems to obtain even 100-120 V/220-240 Vg 0.85/0.45 A 50/60 Hz clearer images. Focusing Stage height movement by roller guide (rack & pinion) •Prolonged active-cell observation with highly reliable data. Stroke per rotation: 36.8 mm •Special microscope body for FV1000 is available. Full stroke range: 25 mm Tension adjustment on coarse focus adjustment knob Upper limit stopper (CX41) Illumination Transmitted Koehler light 12V100W halogen Upper limit stopped by simplified pre-focusing dial (CX31) Focusing Motorized focus; Stroke: 9mm Resolution: 0.1µm Observation tube Binocular/Tilting binocular/Trinocular Observation tube Tilting binocular (F.N. 22), trinocular (F.N. 22) Nosepiece Fixed quadruple nosepiece with inward tilt Nosepiece Motorized sextuple with simple waterproof mechanism Stage Size: 188(W) X 134 (Y) mm Stage Cross with flexible right handle, plain, mechanical Traveling range: 76mm(X) x 50mm(Y) Condenser Motorized long working distance universal, long working distance universal, DIC, mid long working distance, Specimen holder: Double slide holder 8-position universal, ultra long working distance universal Condenser Abbe condenser, with built-in daylight filter(CX31 only) Other features Video port for primary image, Accessories Dual-observation attachment, phase-contrast attachment, integrated magnification change lens drawing attachment, simple polarizing attachment, Accessories Motorized/manual reflected light fluorescence attachment, DIC digital camera adapter etc. attachment, external power supply unit, side-viewing attachment, incubator, heat stage, micromanipulator, etc. CX31 *Please refer to CX41, CX31 catalogs for further details *Please refer to IX71/IX81 catalog for further details CX419 10 The CX21 series microscopes demonstrate the ideal combination Designed to provide the high performance and versatility needed of advanced performance and operability for multiple inspection by researchers involved in live cell experiments, the IX71 offers and educational purposes in the medical field. Incorporating the highly precise temperature control and resistance to heat and BIOLOGICAL MICROSCOPES UIS2 optical system and employing Plan objective lenses as RESEARCH INVERTED SYSTEM MICROSCOPE/ vibration, enabling work on live cells with much less risk of standard, it delivers class-leading standards of image clarity and damage and reducing the incidence of failure in prolonged flatness in a wide range of observation methods. INVERTED SYSTEM MICROSCOPE experiments. Other characteristics include excellent durability, and ergonomic A 30W illumination pillar type IX51, which has no intermediate design features to reduce fatigue during long observations. magnification changer, is also available. To maintain performance in any working environment, an effective anti-fungal treatment is applied to the objectives, eyepieces and •More free space and a better working environment, with flexible use of microscope tube. several cameras and light sources. •Easy front operation allows auxiliary equipment to be placed near the IX51 microscope. Optical system UIS2 (Universal Infinity System) optical system •Flexible system expansion allows many different fluorescence applications Illumination System Built-in transmitted illumination system without major remodeling. 0.5 W LED (CX21LED)/6 V 20 W halogen bulb (CX21) •Obtaining high-quality, purpose-specific images with different cells and Focusing Stage height movement (coarse movement stroke 20 mm) different types of container. Fine focus graduation: 2.5 µm •Rigid construction and comprehensive system features to analyze time Revolving nosepiece Fixed quadruple nosepiece lapse changes in highly active cell conditions. Stage Wire movement mechanical fixed stage: 120 X 132mm Traveling range: 76 mm (X) X 30 mm (Y) Single specimen holder Illumination Transmitted light 12V100WHAL for IX71, 6V30WHAL for IX51 Observation tube 30° inclined binocular tube Focusing Vertical movement of nosepiece (stage fixed); Interpupillary distance adjustment range 48-75 mm coarse & fine coaxial handle; full stroke: 9mm; minimum fine adjustment: 1.2µm Condenser Abbe type with aperture iris diaphragm N.A.: 1.25 Observation tube Tilting binocular (F.N.22), binocular (F.N.22), trinocular (F.N.22) Objective lens Plan Achromatic objectives (anti-fungus) 4X N.A.: 0.10 W.D.: 18.5 mm Nosepiece Sextuple, simple waterproof mechanism 10X N.A.: 0.25 W.D.: 10.6 mm Stage Cross with flexible right handle, cross with short left handle, 40X N.A.: 0.65 W.D.: 0.6 mm plain, mechanical 100X Oil N.A.: 1.25 W.D.: 0.13 mm (option) Condenser Motorized long working distance universal, Eyepiece (10X) Field Number (F.N.): 18 (anti-fungus) long working distance universal, DIC, Optional accessories Mirror unit, 15X eyepiece (F.N. 12, anti-fungus), wooden mid long working distance, 8-position universal storage box, filar micrometer, wire pointer, filter holder, ultra long working distance universal IX51 darkfield stop, cord rest (CX21 only) Other features Video port for primary image, integrated magnification change lens *Please refer to CX21LED/CX21 catalog for further details Accessories Reflected light fluorescence attachment, DIC attachment, external power supply unit, side-viewing attachment, incubator, heat stage, micromanipulator, etc. CX21LED CX21 *Please refer to IX71/IX81 catalog and IX51 catalog for further details IX71
Accessories for IX2 As a joint development with Narishige Scientific lab, the ON3 TIRFM Total internal reflected fluorescence microscopy ON3 Series micromanipulators offer high-precision and easy operation for IVF, injection and physiological experiments. MICROMANIPULATORS An exclusive high N.A. (1.65,1.49) objective and reflected light illuminator are provided, allowing exchange between evanescent wave and normal fluorescence observation. *TIRFM is a class 3B laser product. ON3-99D (1:1) Oil-hydraulic micromanipulator system U-DPCAD Dual port with C-mount adapter The ON3-99D consists of a pair of three-axis motorized positioners, drop handle joystick micromanipulators and UT-D universal joints. This double port tube allows the The ON3-99D is ideal for ICSI applications. attachment of two cameras (both primary Two types of injectors, the IM-9B microinjector (for sperm injection) IX71 TIRFM version images). and the IM-9C pneumatic injector (for oocyte holding) are available separately. Drop handle joystick •Fine movement range: 10mm •Full rotation of knob: 250µm •Minimum graduation: 2.5µm •Joystick (for X and Y movement): 400µm max. (movement ratio and lever tension adjustable) Motorized positioner •Coarse movement range: 23mm (movement speed adjustable) IX2-RFA IX2-RFAL IX2-GS Fluorescence illuminator L-shaped fluorescence illuminator Gliding stage Can be mounted with six different mirror Allows easy centering and AS/FS To follow the quick movement of units. An original mirror unit can be tailor- operation from the front and also permits caenorhabditis elegans, this stage is made from generic mirror units. UV cut attachment of a large format camera to designed to move smoothly and freely filter integrated. the back port. throughout the plane.11 12 IX2-LWUCD IX2-MLWCD IX2-DICD+IX2-TLW Long working distance universal Mid long working distance DIC condenser + condenser condenser water top lens for DICD The new DIC system is especially effective in The relief contrast condenser is designed By combining the IX2-TLW top lens, DIC obtaining high-contrast, high-resolution to produce contrast and shading effects, prism and DIC slider, this model provides images in 20X and 40X DIC observations. similar to DIC, yet within the confines of excellent operability in injection and patch plastic sample vessels. clamping operations. ONO-301D MHW-3 Drop handle joystick micromanipulator (1:1) Three-axis water hydraulic drum type micromanipulator Thanks to its symmetrical design, this micromanipulator can be attached The MHW-3 is useful for physiological experiments as well as fine on either the right or the left side. In combination with the optional universal injection or suction. The solid design including mechanical coarse IX2-TVRAC IX2-RFACA IX2-LWUCDA2 joint (UT-D) and return mechanism (UT-R), it also provides a pipette return Motorized bottom port unit with Motorized fluorescent cube turret Motorized long working distance function. positioner provides reliable stability and precise movement. C-mount universal condenser The optional MHW-4 single axis micromanipulator is mountable. Accepts up to 6 fluorescence filter cubes, making it •Accessory: IP plate Entirely aberration-free primary images Simultaneously accepts up to 6 optical •Fine movement range: X, Y and Z axes 10mm easy to switch between them during fluorescence •Full rotation of knob: 250µm •Fine movement range: 2mm from UIS2 objectives are directed to observation of multi stained specimens. components at maximum. Motorized •Coarse movement range: 30mm •Minimum graduation: 2.5µm exchange through PC possible. •Full rotation of knob: 50µm C-mount CCD camera. (Manual shutter included.) Photo: ONM-2D, ONO-301D, UT-D and UT-R configuration •Minimum graduation: 0.2µm
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