Custom Retrovirus Production from Creative Biogene

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Retroviruses are negative single-stranded RNA viruses in which tRNA serves as a primer for the mRNA synthesis. The mRNA is then reverse transcribed into DNA, which is then integrated into the chromosome at a multiplicity of sites, particularly those that are transcriptionally active. Retroviral vector-mediated gene transfer has been central to the development of gene therapy. 

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Custom Retrovirus Production from Creative Biogene

  1. 1. Custom Retrovirus Production Presented by Creative Biogene About Creative Biogene Creative Biogene provides kits, reagents, and services that help researchers explore questions about gene discovery, regulation, and function. Creative Biogene holds a leadership position in the global market and is committed to improving the human condition through biotechnology.
  2. 2. Introduction • Retroviruses are negative single-stranded RNA viruses in which tRNA serves as a primer for the mRNA synthesis. The mRNA is then reverse transcribed into DNA, which is then integrated into the chromosome at a multiplicity of sites, particularly those that are transcriptionally active. Retroviral vector-mediated gene transfer has been central to the development of gene therapy.
  3. 3. Advantages • Retroviruses have several distinct advantages over other vectors: • transform their single-stranded RNA genome into a double stranded DNA molecule that stably integrates into the target cell genome.
  4. 4. Provider • Creative Biogene is pioneering in the development of retroviral technology with years of experience in retroviral services. Creative Biogene's custom retrovirus production is a highly efficient approach to deliver genes of interest for stable expression into a broad range of dividing mammalian cells and it aims to provide valuable research tools and services in molecular biology.
  5. 5. Custom Retrovirus Construction Services  Construction of ordered retrovirus vector (gene expression and shRNA expression).  Production of a retrovirus producer cell line by transfection.  Production of viral supernatant by transient transfection as well as assay for replication competent retrovirus.  Quality control assays including quantification of genome copies and quantification of retroviral vector infectivity.
  6. 6. Contact Creative Biogene at http://www.creative-biogene.com/Services/Custom-Tel: 1-631-626-9181 or 44-207-048-3343 Email: info@creative-biogene.com
  7. 7. Contact Creative Biogene at http://www.creative-biogene.com/Services/Custom-Tel: 1-631-626-9181 or 44-207-048-3343 Email: info@creative-biogene.com

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