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152 1 31-2011 spectroscopy

by Joanne Cox on Feb 01, 2011

http://be-enviornment-friendly.blogspot.com	6
http://www.be-enviornment-friendly.blogspot.com	6
http://be-enviornment-friendly.blogspot.in	1

Pix of spectra and T and A
Yellow - green
Blues and reds
Gaseous spectra – line spectra
Solution spectra continuous --
Spectrophotometer
Transmittance I/Io X 100%
A = -log (%T/100)
Beer’s Law A = kc
Keep closed
Open on when
necessary
Dark adjust
Blank (water) adjust
Set wavelength
Set dark adjust %T to 0
Insert blank (water test tube)
Set %T to 100
Remove blank, insert sample
Read %T
Repeat every time wavelength is changed
Once you have chosen
Wavelength
Set dark adjust and blank
Once, then check all samples
You may want to
Recheck dark/blank
occasionally