New Uses of Cord Blood

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New Uses of Cord Blood

  1. 1. Mahendra Rao- NIHCRM New Uses of Cord Blood
  2. 2. The Cord Blood Industry Hybrid • Korea Other stem cells MSC and Cord Banking other cells Making iPSC HSC derivatives • Public cord blood units are HLA typed • FACT offers accreditation services • US FDA has required licenses for public banks and we expect this to be extended to private banks • The use of cord blood in children has exceeded the use of bone marrow in children Private eg: • Beike • China Cord Blood • CBR Public eg: • NYCBB • Carolinas • ClinImmune • Private cord blood banks have proliferated and prices for storage have dropped. • Most private large cord blood are in Asia and there is some consolidation and the average units stored in banks is in the range of 100K units • Likely 50% of the units are too small for therapeutic use Expanison of CBB’s T cells, NK cells etc.
  3. 3. Potential other uses of cord blood 1: Expansion of cord blood 2: Identifying other stem cell populations a: MSC b: Hemangioblast c: Endothelial cells d: Differentiated blood products 3: Storing and using Placental tissue and cord 4:: Using infrastructure to store and process other cells 5: Making iPSC 6: Transdifferentiating to other cell types
  4. 4. Name/Website ViviCells International, Inc. http://www.vivicells. com MEDIPOST http://www.medi- post.com/ Lifeline Cord Blood Bank Thai StemLife http://www.thaistemlife.co. th Vita 34 AG http://www.vita34. de/ Cord Blood America http://www.cordblood- america.com Anthrogenesis Inc. Eticur GmbH http://www.eticu r.de Cordlife Group Limited http://www.cordlife. com Meko Stem http://www.mekoste m.com/ MAZE Cord Blood Laboratories http://www.mazecordblo od.com Community Blood Services Public Bank http://www.com munitybloodserv ices.org The Institute For Transfusion Medicine Clinical Services, ITxMCS, Cord Blood Services http://www.givcord.org/ StemCyte Inc. http://www.stemcy teinc.com Cord Blood Bank of Canada Inc http://www.cordbloodb ankofcanada.com AssureImmune LLC http://www.ass ureimmune.co m/ FamilyCord http://www.famil ycord.com/ CReATe Cord Blood Bank http://www.createco rdbank.com MiracleCord, Inc http://www.miraclec ord.com/ Karocell Tissue Engineering http://www.karocell.com NHS Cord Blood Bank http://www.nhs bt.nhs.uk/ Insception http://www.insception.com / Smart Cells International http://www.smartc ells.com/ Cord Blood Registry http://www.cordblood. com BioE, Inc. http://www.bio e.com Future Health Biobank http://www.futur ehealth.co.uk CRYOBANKS INTERNATIONAL, INC. http://www.cryo- intl.com National Stem Cell, Inc. http://www.national stemcell.com Lazaron Biotechnologies (SA) Ltd http://www.lazaron.co.za StemLife Malaysia http://www.ste mlife.com Celgene Corporation http://www.celgene.com PacifiCord http://www.pacific ord.com/ Cord Blood Solutions http://www.cordbloods olutions.com/ Biohellenika http://www.biohe llenika.ro Healthbaby Biotech Co. Ltd http://www.healt hbaby.hk/ Cryo-Cell International, Inc. http://www.cryo- cell.com NeoCells http://www.neocells. com/ Lifebank http://lifebank.com/ StemOne Biologicals Pvt. Ltd. http://www.ste mone.co.in Cells For Life http://www.cellsforlife.com / Progenics Cord Blood Cryobank http://www.progen icscryobank.com Canadian Cord Blood Registry http://www.ccbr.ca BioLife Solutions Inc http://www.biol ifesolutions.co m Healthcord http://www.healt hcord.com/ Cryo-Cell Mexico http://www.cryo- cell.com.mx/ NeoStem, Inc. http://www.neostem .com LifebankUSA, Inc. http://www.lifebankusa.c om Stem Cell Institute http://www.ste mcell.co.jp/ China Cord Blood Corporation http://www.chinacordblood corp.com Relicord http://www.relicor d.com/ Biosafe http://www.biosafe.ch Bionet http://www.bab ybanks.com/20 09/bionet-en Héma-Québec http://www.hem a-quebec.qc.ca Cryocord http://www.cryocord .com.my Netcells Cryogenics http://www.netcells. co.za/ LifeCord International http://english.lifecord.co. kr Stem Sciences, Inc http://www.ste msciences.com/ CorCell, Inc. http://www.corcell.com Saneron CCEL Therapeutics, Inc. http://www.sanero n-ccel.com Alpha Cord http://www.alphacord.c om/ CordBank http://www.cor dbank.co.nz HemaStem http://www.hem astem.com/ Cryolife http://www.cryolife. com.hk New England Cord Blood Bank http://www.cordblo odbank.com Lifeforce Cryobanks http://www.lifeforcecryo banks.com ViaCord http://www.viac ord.com/ Victoria Angel Registry of Hope Public Cord Blood Bank http://www.cellsforlife.com /victoriaangel/ ScienCell Research http://www.scienc ellonline.com Anthony Nolan Trust Cord Blood Bank http://www.anthonynol an.org/ CordBancUSA D-Finitive Cell Technologies Cryo-Save http://www.cryo- save.com New Jersey Cord Blood Bank LifeLine Cryogenics http://www.lifelinecryog enics.com SEWON Cellontech http://www.cell ontech.com Virgin Health Bank http://www.virginhealthban k.com/ Securacell http://www.secura cell.com Alberta Cord Blood Bank http://www.acbb.ca/ Elie Katz Umbilical Cord Blood Program http://www.co mmunitybloods ervices.org/cb_ ekucbp.php Cryosite http://cordblood. cryosite.com/ Cord Blood Companies
  5. 5. ViviCells International, Inc. http://www.vivicells.com MEDIPOST http://www.medi-post.com/ Lifeline Cord Blood Bank Thai StemLife http://www.thaistemlife.co.th Meko Stem http://www.mekostem.com/ MAZE Cord Blood Laboratories http://www.mazecordblood.com Community Blood Services Public Bank http://www.communityblo odservices.org The Institute For Transfusion Medicine Clinical Services, ITxMCS, Cord Blood Services http://www.givcord.org/ MiracleCord, Inc http://www.miraclecord.com/ Karocell Tissue Engineering http://www.karocell.com NHS Cord Blood Bank http://www.nhsbt.nhs.uk/ Insception http://www.insception.com/ National Stem Cell, Inc. http://www.nationalstemcell.com Lazaron Biotechnologies (SA) Ltd http://www.lazaron.co.za StemLife Malaysia http://www.stemlife.com Celgene Corporation http://www.celgene.com NeoCells http://www.neocells.com/ Lifebank http://lifebank.com/ StemOne Biologicals Pvt. Ltd. http://www.stemone.co.in Cells For Life http://www.cellsforlife.com/ NeoStem, Inc. http://www.neostem.com LifebankUSA, Inc. http://www.lifebankusa.com Stem Cell Institute http://www.stemcell.co.jp/ China Cord Blood Corporation http://www.chinacordbloodcorp.com Netcells Cryogenics http://www.netcells.co.za/ LifeCord International http://english.lifecord.co.kr Stem Sciences, Inc http://www.stemsciences.c om/ CorCell, Inc. http://www.corcell.com New England Cord Blood Bank http://www.cordbloodbank.com Lifeforce Cryobanks http://www.lifeforcecryobanks.com ViaCord http://www.viacord.com/ Victoria Angel Registry of Hope Public Cord Blood Bank http://www.cellsforlife.com/victoriaangel / New Jersey Cord Blood Bank LifeLine Cryogenics http://www.lifelinecryogenics.com SEWON Cellontech http://www.cellontech.co m Virgin Health Bank http://www.virginhealthbank.com/ Vita 34 AG http://www.vita34.de/ Cord Blood America http://www.cordblood-america.com Anthrogenesis Inc. Eticur GmbH http://www.eticur.de Cord Blood Companies
  6. 6. StemCyte Inc. http://www.stemcyteinc.com Cord Blood Bank of Canada Inc http://www.cordbloodbankofca nada.com AssureImmune LLC http://www.assureimm une.com/ FamilyCord http://www.familycord.com/ CReATe Cord Blood Bank http://www.createcordb ank.com Smart Cells International http://www.smartcells.com/ Cord Blood Registry http://www.cordblood.com BioE, Inc. http://www.bioe.com Future Health Biobank http://www.futurehealth.co.uk CRYOBANKS INTERNATIONAL, INC. http://www.cryo- intl.com PacifiCord http://www.pacificord.com/ Cord Blood Solutions http://www.cordbloodsolution s.com/ Biohellenika http://www.biohellenik a.ro Healthbaby Biotech Co. Ltd http://www.healthbaby.hk Cryo-Cell International, Inc. http://www.cryo- cell.com Progenics Cord Blood Cryobank http://www.progenicscryoba nk.com Canadian Cord Blood Registry http://www.ccbr.ca BioLife Solutions Inc http://www.biolifesoluti ons.com Healthcord http://www.healthcord.com Cryo-Cell Mexico http://www.cryo- cell.com.mx/ Relicord http://www.relicord.com/ Biosafe http://www.biosafe.ch Bionet http://www.babybanks. com/2009/bionet-en Héma-Québec http://www.hema- quebec.qc.ca Cryocord http://www.cryocord.co m.my Saneron CCEL Therapeutics, Inc. http://www.saneron- ccel.com Alpha Cord http://www.alphacord.com/ CordBank http://www.cordbank.c o.nz HemaStem http://www.hemastem.com Cryolife http://www.cryolife.co m.hk ScienCell Research http://www.sciencellonline.c om Anthony Nolan Trust Cord Blood Bank http://www.anthonynolan.org/ CordBancUSA D-Finitive Cell Technologies Cryo-Save http://www.cryo- save.com Securacell http://www.securacell.com Alberta Cord Blood Bank http://www.acbb.ca/ Elie Katz Umbilical Cord Blood Program http://www.community bloodservices.org/cb_e kucbp.php Cryosite http://cordblood.cryosite.com Cord Blood Companies (con’t)
  7. 7. Blood collection and processing devices
  8. 8. Derivation of an iPSC culture Reprogramming Candidate ESC like line Select clones Adult cells Identify iPSC clones from partially programmed Differentiated cell type Grow propagate and bank Around 1-5M Multiple strategies with advantages and disadvantages Around 21-28 days 1-6 rounds of infection Around 50-150 colonies 10-30 clones picked Pick 3 to be safe per parent line ESC like line Test for differentiation Test for pluripotency Around 8 weeks-12 weeks Around 5 weeks for EB stage Around 6 weeks Around 6 weeks
  9. 9. Why source from cord blood banks 1: Tissue sourcing is well organized and validated 2: HLA typing data is already being collected by banks 3: Existing samples can be used without compromising their ultimate use 4: A renumeration model already exists 5: Zerofootprint methods work well with cord blood cells 6: A Xenofree media and protocol has been developed so clinical grade iPSC lines can be made 7: Cord blood is the youngest source of stem cells one may obtain reliably and easily 8: Stem cells in general have specific mechanisms to maintain genomic integrity , delay senescence and protect against transformation. 9: The process can be easily extended to blood banks and marrow derived CD34 cells using the same infrastructure 10: Somatic memory and differentiation bias may work in our favor for early therapeutic efforts 11: May revive the cord blood banking business Table 1: The relative advantages of making iPSC from either a private or a public cord blood bank are listed.
  10. 10. iPSC from blood or cord blood •Fresh Harvest •CD34+ cells or mononuclear fraction •Confirm consent •Data Anonymization Issues •HLA typing data •iPSC generation •50,000 cells •Sendai •Electroporation •Vectors •IP issues •96 well format •Media and timeline •At time of use of unit •From designed bag or tubing •/ Non Ficoll process •Cord Bank •Receive at iPSC site •Shipping •Tracking •Identity •iPSC testing •Pluripotency •Differentiation •Karyotype •Ship and store
  11. 11. Thank YouCollaborators

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