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Allylix
 

Allylix

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    Allylix Allylix Presentation Transcript

    • Commercial Terpene Production Using Engineered Saccharomyces cerevisiae
      Bio-Based Chemicals East
      Boston, Massachusetts
      September 14, 2010
      1
    • Accelerating Use of Industrial Biotechnology Will Transform the Chemical Market
      Advances in metabolic engineering are enabling the manufacture of diverse products that are impractical to produce synthetically
      Natural or sustainable replacements forexisting petrochemicals
      New sustainable chemicals with improvedor novel properties
      Rapid improvement in yields allows cost-effective production and broad use
      Sustainable biologic processes have lower environmental footprint than their petrochemical counterparts
      Reduction/elimination of toxic byproducts
      Reduced greenhouse gas emissions
      Abundant and renewable raw materials
      2
    • Industrial Biotechnology Market
      3
      Source: USDA
    • Synthetic Biology and Industrial Biotechnology
      4
    • Allylix Technology
      Proprietary metabolical engineering and fermentation platform for the production of rare and chemically complex terpenes
      Optimized production of bio-identical commercial compounds
      Development of novel compounds with valuable properties Advantages:
      Cost effective
      Sustainable supply
      Consistent quality
      Strong patent position
      5
    • Allylix Platform Allows Biosynthetic Production of Commercially Inaccessible Sesquiterpenes
      Sesquiterpenes
      Flavors & Fragrances
      Urban Pesticide
      Crop Protection
      Food Ingredients/Nutraceuticals
      Cosmetic Compounds
      Pharmaceutical Intermediates
      Historically expensive to produce
      Multicyclic, multichiral compounds difficult and expensive to synthesize
      Low natural abundance makes them expensive to extract
      6
      Nootkatone
    • Allylix has Scaled and Commercialized the First Products From its Metabolic Engineering Platform
      7
      Built integrated technology platform and developed pipeline of products
      Scaled fermentation and purification processes to commercial scale
      Commenced commercial product and sales of first two products
      Advanced pipeline of six additional products that address multiple markets
      Flavor and fragrance
      Food ingredient/nutraceutical
      Crop protection/urban pesticides
      Cosmetic chemicals
    • 8
      Sesquiterpene Production in Nature
      Glucose
      Manyenzymatic steps
      (ubiquitous)
      FPP
      Sesquiterpene synthase
      (plant specific)
      Valencene
    • Sesquiterpene Synthase Diversity
      9
    • Allylix Platform for Terpene Production
      Gene Isolation
      Synthesis, informatics, cloning
      Protein engineering
      Generating improved synthases
      Metabolic engineering
      Production of high levels of FPP for conversion to terpenes
      Fermentation
      Economical production of terpenes
      Combinatorial chemobiosynthesis
      Chemical modification of biosynthetic terpenes to produce novel or commercially-inaccessible products
      10
    • Protein Engineering Used to Develop Terpene Synthases with Optimized Specificity and Catalytic Efficiency
      Altering specific amino acids in a terpene synthase to generate one with improved characteristics
      Improved synthases:
      Specificity and selectivity
      Optimize production of desired product
      Catalytic efficiency
      More active, robust enzymes
      11
    • Metabolic Engineering for Cost-Effective Sesquiterpene Production by Fermentation
      12
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      Eliminate bottlenecks in carbon flow to terpenes through:
      • Improved carbon flow from acetyl-CoA to FPP
      • Modulation of squalenesynthase activity
      • High level expression of optimized terpenesynthase
    • Commercial Production
      Fermentation and recovery of first products at commercial scale
      Valencene: Orange flavor and fragrance
      Nootkatone: Grapefruit flavor and fragrance
      13
    • Allylix Fermentation Process Offers a Step Change in the Cost of Production
      14
      When fully developed:
      one commercial scale fermentation
      will produce as much valencene as
      1.2 million metric tons of oranges
    • Discovery of and Screening of Novel Terpenes
      X
      X
      Chemical modification of biosynthetic terpene scaffolds to produce novel or commercially-inaccessible products
      “Smart” libraries built on chiral scaffolds more likely to produce compounds with desirable properties – “hits”
      Natural products produce higher proportion of hits than randomly generated products of combinatorial chemistry
      Natural products more closely resemble bioactive compounds
      Renewed emphasis on natural products screening and screening of semisynthetic natural product libraries
      Y
      Primary Derivatives
      (Dozens per scaffold)
      Secondary Derivatives
      (Hundreds per scaffold)
      Scaffolds
      (Several)
      15
    • Summary
      The accelerating use of industrial biotechnology to produce renewable chemicals will transform the chemical market
      Allylix has scaled and commercialized the first products from its metabolic engineering platform
      Allylix platform technology supports a cost-effective and sustainable supply of commercial terpenes
      Modular platform allows development of new compounds by introduction new synthases into engineered yeast host strains with incremental investments of time, effort, and cost
      New terpenescan be and discovered and produced through generation and screening of chemobiosynthesiclibraries
      16