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Benefits of bioaugmentation
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  • 1. CLOVER ORGANIC PVT. LTD. USE OF NATUREVEL – WW FOR WASTEWATER TREATMENT1. Bio-augmentation: The needBio-augmentation is the application of specifically selected bacteria (microbes) into awastewater treatment system to enhance the system performance in some way. Bio-augmentation has many applications in all types of biological wastewater treatmentsystems, including once-through lagoons, activated sludge plants, sequencing batchreactors (SBRs) and rotating biological contactors (RBCs).2. NatureVel – WW:Though many bacterial cultures are available globally, NatureVel – WW isconsidered the most advanced as: 1. It is the only product that offers the combined benefits of both powerful microbial and herbal extracts. 2. It has a plate count of 25 million microbes as compared to < 5 million microbes in most microbial products. 3. As a result the shelf life is 1 year as compared 6 months in other products.Key Benefits that NatureVel – WW offers are as follows: Meeting CPCB norms much more stringently in terms of BOD, COD, TSS, Coliform count (in sewage). Reduction in cost of treatment through reduced aeration and usage of chemicals and polymers. Decreased environmental liabilities as the water is good enough to be re- cycled. Increase in system reliability and efficiency Massive Sludge Reduction. It takes care of shock loads to some extent. Suppression of foul odour and vectors. Some plants have constantly changing influents. No matter how great a control they have, their biomass is in constant flux. Adding NatureVel - WW helps to stabilize and increase the efficiency of the biomass. When a plant has a system that is old or under-designed, and capital improvements would be too costly, optimization of the current system can be achieved with the addition of NatureVel - WW as a short term help to avoid costly capital improvements. Some plants discharge to a CETP. Addition of NatureVel - WW can decrease the surcharges that are incurred. Sometimes in a once through lagoon, addition of bacteria can shorten recovery time, increase numbers during cold spells or high loading bursts.
  • 2. Without Bioaugmentation With Bioaugmentation3. Conceptual impact of bio-augmentation through NatureVel – WW onbacterial population in wastewater Treatment plantsThe concept of NatureVel -WW is based on the inoculation of mixed cultures ofbeneficial microorganisms into the system where they shift the microbiologicalequilibrium. A series of inoculations are made to ensure that the introducedmicroorganisms continue their dominance over the indigenous populations. Unlikeconventional technologies like the use of UV and chlorination, where reactionarytreatment of unwanted pathogens is carried out, NatureVel – WW on the other handeffectively cultivates beneficial microorganisms to a level where pathogenic,destructive bacteria cannot compete with and therefore do not proliferate. This in turnreduces biological stress of purifying wastewater.The exact mechanism of how NatureVel - WW acts and interacts in the microbialecology is not known. However, there is evidence that supports a number of theoriesconcerning the mechanism of action of NatureVel - WW. These include thesuppression of pathogens and disease-causing microorganisms, conservation ofenergy, solubilization of minerals, microbial-ecological balance, photosyntheticefficiency, and biological nitrification. These microorganisms coexist and actsynergistically when applied. They decompose organic compounds and producevarious low-molecular organic compounds, such as amino acids, sugars, vitamins,enzymes, and other bioactive substances.The unique property of the microbes in NatureVel - WW is that they all work togetherto devour the very toxic compounds caused by human pollution, which throwecosystems into a state of rapid decline. This leads to a reduction in the BOD andconsequently the COD. As the reduction takes place without the use of DO(Dissolved Oxygen), the oxygen level of water is quickly increased with reduced needfor aeration, thereby leading to cost reduction.Also, these microbes create an organic polymer around the suspended particles,thereby increasing settle ability, leading to increased efficiency in cleaningwastewater.
  • 3. Furthermore, as the NatureVel-effluent makes its way through the sewage system, ithas the effect of purifying the liquid sewage it mixes with as well as the sewers as itpasses through. It then goes on to play a similar role in helping to clean up pollutionin rivers when it empties into them.NatureVel – WW microbes remove foul odor from any kind of water contamination ata very fast pace. This is done through five separate ways. These are:1. Odor substances are of weak alkaline nature and are primarily represented by ammonia. It is neutralized with organic acids in NatureVel - WW solution.2. The enzyme and antioxidants reduce odor in a synergistic way, a sort of buffer effect.3. The metallic chelates react with odor substances instantly, change them into non- odor substances and reduce them quickly.4. Foul odor is emitted by putrefactive type of microorganisms. When NatureVel - WW is applied to a local environment and starts to dominate it with its fermentation type of microorganisms, they will stop the process of putrefaction and move towards a fermentation process.5. The herbal extracts in NatureVel – WW help to further clean the wastewater and also suppress foul odour.4. Microbiology:Phototrophic BacteriaMicrobial oxidation of odor compounds, while it can be addressed in separateprocesses (e.g. biofilters), in large part can occur in the aerobic treatment process.Phototrophic bacteria and other species are known sulfide oxidizers under anaerobicconditions (Eaton et al, 1995). Photosynthetic green and purple sulfur bacteria useH2S as a hydrogen donor in photosynthesis. The sulfide is oxidized to sulfur orsulfate. The purple sulfur bacteria has a red appearance that can be seen on clarifierweirs and tops of trickling filter beds, and are known to rarely cause problems intreatment plants. Aerobic bacteria such as Thiobacillus oxidize reduced sulphur toobtain energy for chemoautotrophic growth (Eaton et al, 1995 and Sylvia, 1998).Lactic Acid BacteriaLactic acid bacteria are gram-positive and well-known for obtaining energy throughfermentation. All of these bacteria grow anaerobically, however they can grow in thepresence of oxygen and therefore are considered aerotolerant anaerobes.Fermentation is an internally balanced oxidation-reduction reaction in which someatoms of the energy source become more reduced while others become moreoxidized. Only a small amount of energy is released during fermentation. Most ofthe energy remains in the reduced fermentation product. An example of fermentationby lactic acid bacteria is the catabolism of gluscose: glucose 2 lactic acid C6H12O6 2C3H6O3
  • 4. One important difference between the subgroups of lactic acid bacteria is in the natureof the products formed during the fermentation of sugars. One kind is known ashomofermentative, which produces almost entirely lactic acid. The other kind isheterofermentative, which produces other substances as well, such as, ethanol, CO2and lactate (Brock and Madigan, 1991). There are 8 species of Lactobacillusmicrobes present in NatureVel – WW, which assist in rapid cleaning of wastewater.ActinomycetesActinomycetes are gram-positive filamentous bacteria characterized by branchingfilaments (mycelia), which is similar to growth by fungi. However, the mycelia likefilaments are similar in diameter to bacteria at approximately 1 m. Mostactinomycetes are obligate aerobes, however some are anaerobes. Mostactinomycetes produce spores and they are commonly found in soils, water, andwastewater treatment facilities. Some Actinomycetes, such as Streptomyces producean “earthy” odor that is due to the production of volatile compounds called geosmins.Actinomycetes can degrade polysaccharides, hydrocarbons, and lignin. Someproduce antibiotics (Bitton, 1994).5. Conditions for NatureVel – WW will work for carrying out Bio-augmentation:It is important to note that NatureVel – WW, though an excellent product, cannotwork well unless certain conditions are met. It is our experience that it is better toleave a client in the beginning if his plant does not meet the specifications beforehand. We gain more respect that way.The conditions that need to be kept in mind are:i. Retention time: There should be a minimum retention time of 12 hours. The longer the retention time, the more time the microbes get to do their job. Retention time is calculated from the time the effluent enters the plant and the time it discharges from the plant.ii. COD: 20,000 mg/liter maximumiii. BOD: 10000 mg/liters maximumiv. pH: 4 and above (treat with chemicals initially to increase the pH if necessary)v. Salinity: 20,000 ppm and below.vi. COD/BOD ratio: 4 and below. (In case the ratio is higher, ask us for our special formulation to treat high chemical loads).vii. Presence of Phenols and other toxins: Up to 3% of total flow.viii. Temperature: Mesophillic (25.C to 40.C)ix. Last but not the least. If the ETP/STP is not properly designed or under- designed, then NatureVel – WW can only bring very limited benefit.6. Dosage Pattern for NatureVel –WW:The dosage pattern for NatureVel –WW is divided into two: a. Initial booster dosage to give a large infusion of microbes into the STP/ETP environment.
  • 5. b. Maintenance dosage to keep a minimum balance of beneficial microbes in the environment. The beneficial microbes have a limited life cycle and need to be constantly replenished. Moreover, with constant flow of pollution into the environment, there needs to be a minimum level of microbes to treat the same.The procedure for dosing NatureVel – WW microbes into the STP/ETP is asfollows:a. The concentrate solution needs to be multiplied 20 times anaerobically in a Jerry Can or for large usage in Sintex plastic Tanks. The procedure for the same is as follows. These same instructions are printed on every bottle of NatureVel – WW for ease of use.b. Care should be taken that the container used for multiplication was not used for any chemicals that may harm the microbes in NatureVel –WW. After multiplication, the containers must be thoroughly cleaned with water and not soap or detergents.c. Once multiplied, you need to dose the plant in the ratio of 1 liter of multiplied culture for every 10,000 liters of effluent or raw sewage. This ratio is generally maintained for both Booster and Maintenance dosage.d. There is no hard and fast rule on this. Please remember that EACH plant is unique in itself. We have in fact seen that similar plants with same flow rate behave differently while under going bio-augmentation. Change dosage slightly to improve performance.e. The indications that the plant is responding well to NatureVel – WW are as follows: As a first step, the foul odour will subside to a large extent. The MLSS would slowly start to build up. The BOD, COD levels will start to reduce, while DO levels will start to rise. The sludge from the bottom of the plant would start to come to the surface and begin to break up into smaller pieces and slowly disappear.
  • 6. f. If the above signs are not appearing, then you need to check the following: The pH level of Multiplied NatureVel – WW is 3.5 and below or not. If not, then there has been some contamination, which has entered or air has entered the anaerobic environment. Throw away the batch and make a fresh batch, but keep in mind the phyto-sanitation conditions. Ensure there has been no shock dosage into the plant. Ensure that no anti-microbial compounds like Chlorides or Phenols have entered the plant. If the BOD in the plant increases after the dosage, then reduce the dosage a little. For a short time, the BOD/COD might increase initially. Do not worry. The native harmful microbes resist the entry of powerful beneficial microbes in NatureVel – WW. The plant should stabilize within 7 days.