Agrosalud Breeding Sweetpotato WG 2009
Biofortified Sweetpotato
Available Material, Advances, and Selection Limits
Interna...
General: There were orange fleshed
sweetpotatos (OFSP) before
The Base Lines for β-carot., Fe & Zn.
Example: Data for 6 Me...
AgroSalud Sweetpotato Breeding WG 2009
Sweet Potato Targeting - Groupings by Opportunities and
Number of People – Where is...
Germplasm Clones in Dissemination (phase I)
2006 – 2007 Screening of 1200 clones >3 locations
AgroSalud Sweetpotato Breedi...
AgroSalud Sweetpotato Breeding WG 2009
ppm DM
2005 - 2006
YLD
(t / ha)
DM
(%) β-carot. Fe Zn
PJ I Pop. Mean 13.5 30.0 79 2...
Breeding Clones in Dissemination (phase II)
2009 =>
AgroSalud Sweetpotato Breeding
WG 2009
ppm DMN = 182
>150 ppm β-carot
...
Variety Release (CIP Peru – old clones crossed before Phase I)
AgroSalud Sweetpotato Breeding WG 2009
ppm DMYLD
(t / ha)
D...
CIP Field days in suburbs Lima 2006 – 2009 => 2012
linked with the”Comedores Populares”, the “Municipalidad de Carabayllo”...
Further Advances for OFSP Breeding !!!
1) New Information that iron & zinc might be improved by
50% in sweetpotato to: 40 ...
New Infromation Fe & Zn in sweetpotato (8 x 8 diallel)
Note: crossings with 8 outstanding PJ I clones (in total 64 familie...
Agro Salud Sweetpotato Breeding WG 2009
SPCSV Resistance
CIP 420269 PER (DLP 3163) selected from 2000
germplasm clones as ...
AgroSalud Sweetpotato Breeding WG 2009
Sources of weevil tolerance
Not made at CIP!!! But of high interest for LAC!!!
Inde...
Thank-you for your Attention
AgroSalud Sweetpotato Breeding WG 2009
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Biofortified Sweetpotato

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Wolfgang Gruneberg
Biofortified Sweetpotato Available Material, Advances, and Selection Limits
Reuión anual de socios y colaboradores de AgroSalud / 2009

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Biofortified Sweetpotato

  1. 1. Agrosalud Breeding Sweetpotato WG 2009 Biofortified Sweetpotato Available Material, Advances, and Selection Limits International Potato Center
  2. 2. General: There were orange fleshed sweetpotatos (OFSP) before The Base Lines for β-carot., Fe & Zn. Example: Data for 6 MegaClones (from 20 MegaClones) AgroSalud Sweetpotato Breeding WG 2009 ppm DMYLD (t / ha) DM (%) β-carot. Fe Zn Jewel 23.9 27.7 296 23.7 13.1 Resisto 26.8 27.3 533 29.9 18.4 Jonathan 14.7 29.2 158 28.2 16.2 Santo Amaro 16.3 31.7 0 23.3 13.7 Xushu18 30.5 31.2 0 25.2 14.1 Tanzania 11.6 33.5 0 22.5 13.5 Jonathan 100g fresh roots: β-carotene: 4.6 mg, iron: 0.82 mg, zinc: 0.47 mg Child 1 – 3 years needs: β-carotene: 4.8 mg, iron: 5 mg, zinc: 4 mg Note on poor on-farm conditions you have about 30% less β-carotene, iron, and zinc
  3. 3. AgroSalud Sweetpotato Breeding WG 2009 Sweet Potato Targeting - Groupings by Opportunities and Number of People – Where is Latin America?
  4. 4. Germplasm Clones in Dissemination (phase I) 2006 – 2007 Screening of 1200 clones >3 locations AgroSalud Sweetpotato Breeding WG 2009 ppm DMN = 78 clones >150 ppm β-carot YLD (t / ha) DM (%) β-carot. Fe Zn Means (N = 78) 19.5 28.4 262 18.4 11.5 Number of clones > better than 29.2 % DM (check Jonathan) - - - - - N = 25 >150 ppm β-carot YLD (t / ha) DM (%) ppm DM β-carot Fe Zn Means (N = 25) 21.8 29.8 282 23.3 12.7 N = 13 clones >29.2 % DM (check Jonathan) 19.0 31.8 313 22.6 12.0 Breeding Clones in Dissemination (phase I) 2006 – 2007 Screening 5000 clones 3 locations => Comment: There are much better clones, but: huge problems to get clones virus clean and into other countries 2005 -2007 Germination under sterile conditions Controlled crossings Near Infra Red to determine Quality
  5. 5. AgroSalud Sweetpotato Breeding WG 2009 ppm DM 2005 - 2006 YLD (t / ha) DM (%) β-carot. Fe Zn PJ I Pop. Mean 13.5 30.0 79 21 9 Min – Max 0.1– 89.0 16.0- 45.3 0.0 - 398 3 – 40 5 -15 PZ I Pop. Mean 14.0 32.25 123.3 17.8 10.9 Min – Max 0.1 - 50.0 10.1 – 47.9 0 - 863 7.6 – 30.5 4.7 – 19.9 2007 - 2008 PJ II Pop. Mean 19.7 28.2 363 22.3 13.3 Min – Max 2.7 - 44.9 14.5 - 42.6 0 – 1066 9.9 - 45.9 7.0 - 31.0 PZ II Pop. Mean 15.4 30.5 258 18.3 11.1 Min – Max 3.6 - 54.8 16.2 - 45.5 0 - 777 8.1 - 41.0 5.7 – 27.4 OFSP Population Development – 2 Recurrent Cycles Populations: Jewel (PJ) and ZapalloSPK (PZ) from 2005 - 2008 (3 locations !!!) Means, minimum and maximum yield, dry matter, β-carotene, Fe and Zn in Population, Jewel I, II, and Population ZapalloSPK I and II. PJ II 100g fresh storage root mean: β-carotene: 10.2 mg, iron: 0.64 mg, zinc: 0.38 mg PZ II 100g fresh storage root mean: β-carotene: 7.9 mg, iron: 0.56 mg, zinc: 0.34 mg Child 1 – 3 years needs per day: β-carotene: 4.8 mg, iron: 5 mg, zinc: 4 mg
  6. 6. Breeding Clones in Dissemination (phase II) 2009 => AgroSalud Sweetpotato Breeding WG 2009 ppm DMN = 182 >150 ppm β-carot YLD (t / ha) DM (%) β-carot. Fe Zn Means (N = 182) 20.6 28.2 355 21.0 13.2 Means of 59 clones DM & β-carot. Equal or better than (check Jonathan) 20.6 31.7 299 19.5 12.3 Family YLD (t / ha) DM (%) ppm DM β-carot Fe Zn PJ05.042 x 194541.48 22.3 21.2 599 27.7 18.9 SR01.018 x PJ05.056 24.4 28.5 414 25.0 15.4 PJ05.042 x PJ05.172 20.1 27.3 527 24.9 15.8 PZ06.006 x PZ06.099 15.5 29.2 480 19.2 12.5 Breeding SEED in Dissemination (phase II) 2009 => 4 out of 59 high dry matter clones appear to be non sweet sweetpotato
  7. 7. Variety Release (CIP Peru – old clones crossed before Phase I) AgroSalud Sweetpotato Breeding WG 2009 ppm DMYLD (t / ha) DM (%) β-carot. Fe Zn Jonathan (check) 59.5 28.4 302 23.3 13.2 LM03.062 34.3 24.9 288 24.4 14.4 SR02.164 23,7 25.7 287 13.3 9.8 SR02.177 51.3 27.8 525 21.3 13.2 SR02.179 49.5 24.7 756 23.1 14.3 SR02.194 26.7 29.4 339 14.6 8.7 Germplasm clones or Clones crosses 2005 or before INIA, Phase I clones: 2 clones in variety release EMBRAPA, Phase I clones: 4 clones in variety release and dissemination ISA; Phase I clones: 1 clones in variety release ORE; - 2 local clones in dissemination INIVIT (without funds from CIP) 1 local breeding clone released Two further contract to evaluate Phase I clones – UNA & CENTA Clones crosses 2006 - 2007 INIA & CIP, Phase II: About 250 PJ I and PZ Clones in variety development
  8. 8. CIP Field days in suburbs Lima 2006 – 2009 => 2012 linked with the”Comedores Populares”, the “Municipalidad de Carabayllo” & the “Ministerio de Mujers” Image of sweetpotato “No solo para …” AgroSalud Sweetpotato Breeding WG 2009
  9. 9. Further Advances for OFSP Breeding !!! 1) New Information that iron & zinc might be improved by 50% in sweetpotato to: 40 ppm Fe & 25 ppm Zn 2) Source of SPVD resistance (SPCSV resistance) found in 1 OFSP land races and at least 2 clones of PJ I 3) Against other opinions there might be a certain amount of weevil resistance in sweetpotato, which has been associated to specific compounds in the latex of the root skin in clones like New Kawogo or Santo Amaro AgroSalud Sweetpotato Breeding WG 2009
  10. 10. New Infromation Fe & Zn in sweetpotato (8 x 8 diallel) Note: crossings with 8 outstanding PJ I clones (in total 64 families each with 30 to 60 genotypes, each genotype planted in 1 m row plot in 2 plot replications) –only the best 8 families for iron are presented with their mean and standard deviation in brackets AgroSalud Sweetpotato Breeding WG 2009 ppm DM Families DM (%) β-carot. Fe Zn 106806 Mean STD 26.4 (5.3) 730 (351) 40.5 (8.9) 26.5 (6.6) 106765 Mean STD 23.9 (3.7) 686 (174) 39.3 (5.3) 26.2 (3.8) 106770 Mean STD 26.8 (5.0) 516 (374) 37.8 (9.2) 25.3 (6.2) 106778 Mean STD 24.8 (4.2) 781 (301) 37.7 (7.6) 24.7 (5.4) 106816 Mean STD 22.8 (2.7) 725 (143) 37.4 (5.1) 25.3 (4.0) 106786 Mean STD 21.8 (3.1) 586 (209) 37.2 (6.5) 24.6 (5.0) 106814 Mean STD 23.4 (4.5) 617 (294) 36.8 (8.2) 24.4 (6.0) 105764 Mean STD 21.6 (4.3) 732 (231) 36.6 (7.3) 26.0 (5.4) R = Mean + STD Approx. current OFSP selection limits ≈ 30 % DM ≈ 900 ppm pro vitamin A ≈ 45 ppm Fe ≈ 30 ppm Zn R = Mean + 2 STD Approx. future OFSP selection limits ≈ 32 % DM ≈ 1000 ppm pro vitaminA ≈ 50 ppm Fe ≈ 35 ppm Zn
  11. 11. Agro Salud Sweetpotato Breeding WG 2009 SPCSV Resistance CIP 420269 PER (DLP 3163) selected from 2000 germplasm clones as resistant to sweet potato clorotic stunt (SPCSV) Assumption: recessive inherited rrrrrr Crossing with parents of OFSP “Pop. Jewel” DLP 3163 x 100 OFSP “Jewel” Table: Elisa scores in population Jewel before and after crossing with DLP3163 (CIP 420269) *** significant on p < 0.001 level SPFMV SPCSV SPVD (ambos virus) Application of Markers ELISA score Mean ELISA score Median SPCSV SPFMV SPCSV SPFMV Parents 0.84 1.97 0.78 2.11 Offspring 0.44*** 1.09*** 0.33*** 1.00*** When you have a technique to search you can find: We found at least 5 more clones (OFSP) with resistance to SPCSV => Crossings !!!! >5 x > 5 SPCSV resistance clones Number of seed expected >2000
  12. 12. AgroSalud Sweetpotato Breeding WG 2009 Sources of weevil tolerance Not made at CIP!!! But of high interest for LAC!!! Independent reports from Dom. Republic – clone Santo Amaro (Brazil) & Uganda – clone New Kawogo ⇒ less effected by weevil due to differences in latex content and composition Mean No. of feeding holes (feedh), oviposited eggs & dropping on Tanzania (TZ) and New Kawogo (NK) Periderms. 0 2 4 6 8 Feedh Eggs Dropp MeanNo. TZ NK HPLC chromatograms of NK and TZ root latex (40mg latex/ ml
  13. 13. Thank-you for your Attention AgroSalud Sweetpotato Breeding WG 2009

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