Topic 2 Cells
Cell theory <ul><li>All living things are made up of cells </li></ul><ul><li>Cells are the smallest unit of life </li></ul...
What is the evidence for the cell theory ? <ul><li>All living things are made up of cells </li></ul><ul><li>All 5 kingdoms...
What is the evidence for the cell theory ? <ul><li>Tissues are made up of individual cells that can be isolated </li></ul>...
What is the evidence for the cell theory ? <ul><li>Mitosis and meiosis have been observed many times in many different org...
Exceptions to the rule <ul><li>Muscle cells- have lost the membrane between them and so are long cells with many nuclei </...
More exceptions….. <ul><li>Virus is non cellular, just some DNA or RNA surrounded by a protein coat…there is debate about ...
Unicellular organisms <ul><li>Some organisms like many protista and all prokaryotes are only one cell big but they are abl...
 
Amoeba on the move!
Diatoms
Multicellular cells show  emergent properties Emergent properties  are ones that come about when individual parts of a sys...
Think of these 3 parts: 1. A small metal cup 2. A glass bowl 3. A piece of tugnsten wire Individually they have no functio...
<ul><li>Relative sizes: </li></ul><ul><li>Molecules (1nm) </li></ul><ul><li>Cell membrane thickness (10nm) </li></ul><ul><...
<ul><li>Why do cells not grow to larger sizes? </li></ul><ul><li>Answer! </li></ul><ul><li>Surface-area to Volume Ratio! <...
Conclusion Large organisms, the rate of exchange of substances with their surroundings occurs more slowly Cell radius (r) ...
Benefits of Light Microscope Benefits of Electron Microscope <ul><li>Can see things in colour </li></ul><ul><li>Can look a...
Comparisons of microscopes: Light microscope is more limited by its resolving power but allows observation of living organ...
Looking at cells: the microscope <ul><li>Two factors when evaluating a microscope: </li></ul><ul><li>Resolving power : a m...
Units of size
Calculating magnification <ul><li>When you see an image like the ones on the last slide of cells (called a micrograph) the...
<ul><li>Example: </li></ul><ul><li>Use the scale bar on the micrograph. 1um measures 9mm </li></ul><ul><li>Convert the 2 m...
<ul><li>To determine the actual size of something on the micrograph: </li></ul><ul><li>Measure it-example the mitochondria...
Estimating with help <ul><li>You can also estimate the size of a cell or organelle by using a very small ruler that is etc...
Virtual Microscope Lab! <ul><li>http://www.udel.edu/biology/ketcham/microscope/scope.html </li></ul><ul><li>http://nhscien...
Practice with the microscope <ul><li>First go through the virtual microscope tour. We do not have binocular microscopes, o...
Cell differentiation <ul><li>All multicelluar organisms  start out from one cell. </li></ul><ul><li>All cells in an organi...
Cell differentiation <ul><li>This quality of cells in different tissues having different structure and function is the res...
Stem cells <ul><li>Stems cells are unspecialized cells. They can reproduce indefinitely and have the capability of differe...
Embryonic stem cells/adult stem cells
 
Uses of stem cells <ul><li>Tissue repair </li></ul><ul><li>Treatment of disease- Parkinson’s disease, multiple sclerosis, ...
Why are cells small? <ul><li>As the size of a sphere increases, the volume increases more than the surface area.  </li></u...
Two kinds of cells:  prokaryotic  and  eukaryotic Prokaryotic Small- 1 micron Lack nucleus and intermembranous systems Hav...
Two kinds of cells:  prokaryotic  and  eukaryotic Prokaryotic Eukaryotic Small- 1 micron Larger-10-100 microns Lack nucleu...
Prokaryotic cells <ul><li>Draw a diagram of the ultrastructure of  Escherichia coli . Include: </li></ul><ul><li>Cell wall...
Generalized prokaryotic cell
Functions  <ul><li>Cell wall : made of peptidglcans (sugars and proteins),rigid structure, protects cell gives it shape, p...
Prokaryotic cells divide by binary fission
EUKARYOTIC CELLS
 
NUCLEUS
nucleus <ul><li>Structure: </li></ul><ul><li>Double membrane, continuous with ER </li></ul><ul><li>Contains DNA and protei...
RIBOSOMES
ribosomes <ul><li>Structure: </li></ul><ul><li>Made of rRNA and protein </li></ul><ul><li>Small and large subunits </li></...
ENDOMEMBRANE SYSTEM Endoplasmic reticulum
membrane system <ul><li>Structure: </li></ul><ul><li>Made of a phospholipid bilayer similar to the membrane around the cel...
endoplasmic reticulum <ul><li>Smooth ER has no ribosomes, has enzymes embedded in the membrane </li></ul><ul><li>Rough ER ...
Golgi Apparatus
Golgi apparatus <ul><li>Structure: </li></ul><ul><li>Flattened, membranous sacs called cisterns </li></ul><ul><li>The insi...
Lysosome
lysosome <ul><li>Structure: </li></ul><ul><li>Membranous sac with digestive enzymes </li></ul><ul><li>Only in animal cells...
 
vacuoles <ul><li>Similar to lysosomes, have hydrolytic enzymes </li></ul><ul><li>Present in plant and fungi, protista </li...
 
mitochondria <ul><li>Structure: </li></ul><ul><li>Double membrane </li></ul><ul><li>Inner membrane has infolds called cris...
 
chloroplast <ul><li>Structure </li></ul><ul><li>Double membrane </li></ul><ul><li>Inner compartment made up of another int...
Extracellular components <ul><li>Plant cells have a cell wall made of cellulose </li></ul><ul><li>Helps maintain shape, pr...
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Cells PPt

  1. 1. Topic 2 Cells
  2. 2. Cell theory <ul><li>All living things are made up of cells </li></ul><ul><li>Cells are the smallest unit of life </li></ul><ul><li>All cells come from other cells </li></ul>
  3. 3. What is the evidence for the cell theory ? <ul><li>All living things are made up of cells </li></ul><ul><li>All 5 kingdoms of life are made up of cells </li></ul><ul><li>Some organisms are only one-celled, unicellular </li></ul><ul><li>We can observe cells under the microscope </li></ul>
  4. 4. What is the evidence for the cell theory ? <ul><li>Tissues are made up of individual cells that can be isolated </li></ul><ul><li>Cells can live in vitro but organelles can’t. </li></ul><ul><li>Cells are the smallest unit of life </li></ul>
  5. 5. What is the evidence for the cell theory ? <ul><li>Mitosis and meiosis have been observed many times in many different organisms </li></ul><ul><li>Mixing the components of cells together has never resulted in the formation of a cell </li></ul><ul><li>Cells arise from other cells </li></ul>
  6. 6. Exceptions to the rule <ul><li>Muscle cells- have lost the membrane between them and so are long cells with many nuclei </li></ul><ul><li>Fungi cells- also very long and multi-nucleated </li></ul>
  7. 7. More exceptions….. <ul><li>Virus is non cellular, just some DNA or RNA surrounded by a protein coat…there is debate about whether it is alive or not. </li></ul><ul><li>Mitochondria and chloroplasts have their own DNA and can replicate themselves but they are organelles not cells. They are believed to have once been free living prokaryotic cells that formed a symbiotic relationship with a eukaryotic cell. </li></ul>
  8. 8. Unicellular organisms <ul><li>Some organisms like many protista and all prokaryotes are only one cell big but they are able to carry out all the functions of life. </li></ul><ul><li>a. metabolism which includes respiration the synthesis of ATP. </li></ul><ul><li>b. response to a change in the environment </li></ul><ul><li>c. homeostasis the maintenance and regulation of internal cell conditions. </li></ul><ul><li>d. growth which for a unicellular organism means an increase in cell size and volume. </li></ul><ul><li>e. reproduction which for the unicellular organism is largely asexual through cell division to form a clone. </li></ul><ul><li>f. nutrition which means either the synthesis of organic molecules or the absorption of organic matter. </li></ul>
  9. 10. Amoeba on the move!
  10. 11. Diatoms
  11. 12. Multicellular cells show emergent properties Emergent properties are ones that come about when individual parts of a system interact with each other. On their own, the individual parts cannot do what they can do together. The whole is greater than the sum of its parts. A cell can do much more than the individual parts or organelles can do. A protein can do more than the amino acids that its made up of.
  12. 13. Think of these 3 parts: 1. A small metal cup 2. A glass bowl 3. A piece of tugnsten wire Individually they have no function, but when they are together:
  13. 14. <ul><li>Relative sizes: </li></ul><ul><li>Molecules (1nm) </li></ul><ul><li>Cell membrane thickness (10nm) </li></ul><ul><li>Virus (100nm) </li></ul><ul><li>Bacteria (1µm) </li></ul><ul><li>Organelles (less than 10µm) </li></ul><ul><li>Cells (less than 100µm) </li></ul><ul><li>Generally plant cells are larger than animal cells </li></ul>
  14. 15. <ul><li>Why do cells not grow to larger sizes? </li></ul><ul><li>Answer! </li></ul><ul><li>Surface-area to Volume Ratio! </li></ul><ul><li>Rate of heat and waste production and rate of resource consumption are functions that depend on its volume </li></ul><ul><li>Most chemical reactions takes place inside the cell and its size affects the rate of these reactions </li></ul><ul><li>The rate of exchange of substances therefore depends on the organisms surface area that is in contact with the surroundings </li></ul><ul><li>As the organism gets bigger, their volume and surface area both get bigger, but not by the same amount </li></ul>
  15. 16. Conclusion Large organisms, the rate of exchange of substances with their surroundings occurs more slowly Cell radius (r) 0.25 units 0.5 units 1.25 units Surface area 0.79 units 3.14 units 7.07 units Volume 0.06 units 0.52 units 1.77 units SA: Volume 13.17:1 6.04:1 3.99:1
  16. 17. Benefits of Light Microscope Benefits of Electron Microscope <ul><li>Can see things in colour </li></ul><ul><li>Can look at live organisms </li></ul><ul><li>Much cheaper to buy & easy to operate </li></ul><ul><li>Greater magnification </li></ul><ul><li>Great resolution </li></ul>
  17. 18. Comparisons of microscopes: Light microscope is more limited by its resolving power but allows observation of living organisms, movement and processes Transmission electron microscope is used to study the internal structure of the cell Scanning electron microscope is used to study the surface of a sample
  18. 19. Looking at cells: the microscope <ul><li>Two factors when evaluating a microscope: </li></ul><ul><li>Resolving power : a measure of the clarity of the image, the minimum distance two points can be and still be seen as two separate points </li></ul>2. Magnification : how much bigger the image is, the ratio between the actual size and the image. The limiting factor for a microscope is the resolving power. For light microscopes the shortest wavelength of light enables discrimination of 0.2 microns, the size of a bacteria, magnification is limited to 1000X Electron microscopes use electrons and so their resolving power is 0.1 nanometers
  19. 20. Units of size
  20. 21. Calculating magnification <ul><li>When you see an image like the ones on the last slide of cells (called a micrograph) they appear bigger than they actually are because they are magnified </li></ul><ul><li>You need to know how to calculate the magnification and the actual size of the specimen from a micrograph </li></ul><ul><li>Magnification=size of image/size of specimen </li></ul><ul><li>Size of specimen= size of image/magnification </li></ul>
  21. 22. <ul><li>Example: </li></ul><ul><li>Use the scale bar on the micrograph. 1um measures 9mm </li></ul><ul><li>Convert the 2 measurements to the same units </li></ul><ul><li>There are 1000um in 1mm, so 9mm= 9000um </li></ul><ul><li>Plug into the formula: </li></ul><ul><li>M= image size/specimen size </li></ul><ul><li>9000/1=9000X </li></ul>
  22. 23. <ul><li>To determine the actual size of something on the micrograph: </li></ul><ul><li>Measure it-example the mitochondria is 11mm and then use the formula: </li></ul><ul><li>Size=image/magnification </li></ul><ul><li>11,000um/9000=1.2 um </li></ul><ul><li>Is this a reasonable size for an organelle? </li></ul>
  23. 24. Estimating with help <ul><li>You can also estimate the size of a cell or organelle by using a very small ruler that is etched into a microscope slide, called a micrometer. </li></ul><ul><li>You can measure the size of the field of view under different magnifications and use this to estimate the size of something under the microscope. </li></ul>
  24. 25. Virtual Microscope Lab! <ul><li>http://www.udel.edu/biology/ketcham/microscope/scope.html </li></ul><ul><li>http://nhscience.lonestar.edu/biol/dropdrag/index.htm </li></ul><ul><li>http://nhscience.lonestar.edu/biol/dropdrag/micro.htm </li></ul>
  25. 26. Practice with the microscope <ul><li>First go through the virtual microscope tour. We do not have binocular microscopes, or light rheostat, otherwise same. Follow directions- you will get a green check when you have done the task successfully. View an image under 3 powers, adjusting light and focus. </li></ul><ul><li>Then do the activity </li></ul>
  26. 27. Cell differentiation <ul><li>All multicelluar organisms start out from one cell. </li></ul><ul><li>All cells in an organism (except gametes) have the exact same genetic information which guides all the structure and functions of the organism. </li></ul><ul><li>How how is it that a skin cell looks totally different than a nerve cell and that they do completely different things? </li></ul>
  27. 28. Cell differentiation <ul><li>This quality of cells in different tissues having different structure and function is the result of cell differentiation. </li></ul><ul><li>This difference is not due to a difference in the DNA or genes but in how the genes are controlled or expressed. </li></ul><ul><li>Once a cell has developed into a certain kind of cell it cannot be reprogrammed to become another kind of cell. </li></ul>
  28. 29. Stem cells <ul><li>Stems cells are unspecialized cells. They can reproduce indefinitely and have the capability of differentiation. </li></ul><ul><li>They have the ability to become any kind of cell. This is called totiopotency. Cells that can become almost any kind of cell are called pluripotent. </li></ul><ul><li>Human embryo is made up of stem cells up about 5 days old- called the blastula stage. </li></ul><ul><li>These cells can be made to differentiate into almost any cell type. This is why they are the focus of so much research. </li></ul><ul><li>Adults also have stem cells- these can’t make any kind of cell but they can make many different kinds. Ex: bone marrow stem cells can make all the different kinds blood cells as well as bone and muscle cells. </li></ul>
  29. 30. Embryonic stem cells/adult stem cells
  30. 32. Uses of stem cells <ul><li>Tissue repair </li></ul><ul><li>Treatment of disease- Parkinson’s disease, multiple sclerosis, leukemia </li></ul><ul><li>Possible growth of organs </li></ul><ul><li>http://learn.genetics.utah.edu/units/stemcells/whatissc/ </li></ul>
  31. 33. Why are cells small? <ul><li>As the size of a sphere increases, the volume increases more than the surface area. </li></ul><ul><li>The surface area to volume ratio is important because materials must enter and leave the cell through the cell membrane. If the volume of the cell gets too big, the surface area can’t keep up with exchange of materials needed to support the metabolic activities of the cell. </li></ul>So cells maintain a high surface area-to-volume ratio
  32. 34. Two kinds of cells: prokaryotic and eukaryotic Prokaryotic Small- 1 micron Lack nucleus and intermembranous systems Have cell wall DNA: one main circular chromosome and additional DNA in plasmid
  33. 35. Two kinds of cells: prokaryotic and eukaryotic Prokaryotic Eukaryotic Small- 1 micron Larger-10-100 microns Lack nucleus and intermembranous systems Organelles bound by membranes Have cell wall May have cell wall DNA: one main circular chromosome and additional DNA in plasmid DNA: in the nucleus, double stranded, no plasmid
  34. 36. Prokaryotic cells <ul><li>Draw a diagram of the ultrastructure of Escherichia coli . Include: </li></ul><ul><li>Cell wall </li></ul><ul><li>Plasma membrane </li></ul><ul><li>Cytoplasm </li></ul><ul><li>Pilli </li></ul><ul><li>Flagella </li></ul><ul><li>Ribosomes </li></ul><ul><li>Nucleiod region </li></ul>
  35. 37. Generalized prokaryotic cell
  36. 38. Functions <ul><li>Cell wall : made of peptidglcans (sugars and proteins),rigid structure, protects cell gives it shape, prevents entry of water </li></ul><ul><li>Plasma membrane : regulates what goes in and out of cell </li></ul><ul><li>Cytoplasm: fluid inside cell, has enzymes, metabolic reactions </li></ul><ul><li>Pili : hair like structures that can move in and out of cell. Allows one cell to attach to another </li></ul><ul><li>Flagellum : made of proteins provides locomotion in some bacteria </li></ul><ul><li>Ribosomes : protein synthesis </li></ul><ul><li>Nucleoid region : DNA located here (genetic info), no nuclear membrane </li></ul>
  37. 39. Prokaryotic cells divide by binary fission
  38. 40. EUKARYOTIC CELLS
  39. 42. NUCLEUS
  40. 43. nucleus <ul><li>Structure: </li></ul><ul><li>Double membrane, continuous with ER </li></ul><ul><li>Contains DNA and proteins </li></ul><ul><li>DNA wound around proteins organized into chromosomes </li></ul><ul><li>Perforated by pores </li></ul><ul><li>Nucleolus inside </li></ul><ul><li>Function: </li></ul><ul><li>DNA is code for proteins, genetic information </li></ul><ul><li>Nucleolus made up of ribosomal RNA, which makes up the ribosomes where protein synthesis happens </li></ul><ul><li>Pores allow mRNA and rRNA to leave nucleus </li></ul>
  41. 44. RIBOSOMES
  42. 45. ribosomes <ul><li>Structure: </li></ul><ul><li>Made of rRNA and protein </li></ul><ul><li>Small and large subunits </li></ul><ul><li>Some are attached to endomembranous system, some are free </li></ul><ul><li>Function </li></ul><ul><li>Site of protein synthesis </li></ul><ul><li>Proteins made on bound ribosomes are secreted outside the cell </li></ul><ul><li>Proteins made on free ribosomes are used inside the cell. </li></ul>
  43. 46. ENDOMEMBRANE SYSTEM Endoplasmic reticulum
  44. 47. membrane system <ul><li>Structure: </li></ul><ul><li>Made of a phospholipid bilayer similar to the membrane around the cell </li></ul><ul><li>Organelles are surrounded by membranes </li></ul><ul><li>It goes from the nuclear envelope out to the edge of the cell </li></ul><ul><li>Functions: </li></ul><ul><li>Regulation of protein traffic from the ER to vesicles </li></ul><ul><li>Metabolic functions: the inner membranes of mitochondria and chloroplasts </li></ul>
  45. 48. endoplasmic reticulum <ul><li>Smooth ER has no ribosomes, has enzymes embedded in the membrane </li></ul><ul><li>Rough ER has ribosomes </li></ul><ul><li>Smooth ER: synthesis of lipids, metabolism of carbohydrates, detoxification of drugs and poisons. </li></ul><ul><li>Stores Ca++ in muscle cells </li></ul><ul><li>Rough ER: synthesis of proteins and glycoproteins </li></ul><ul><li>Makes membrane proteins and phospholipids that makes up its own membrane </li></ul>
  46. 49. Golgi Apparatus
  47. 50. Golgi apparatus <ul><li>Structure: </li></ul><ul><li>Flattened, membranous sacs called cisterns </li></ul><ul><li>The inside of each sac is kept separate from the cytosol </li></ul><ul><li>Two faces: cis and trans. cis faces the ER and trans faces the outside of the cell </li></ul><ul><li>Function: </li></ul><ul><li>Products from the ER come to the cis face in vesicles </li></ul><ul><li>Proteins and phospholipids are modified </li></ul><ul><li>Makes polysaccharides and other macromolecules </li></ul><ul><li>Products leave from the trans face in vesicles, these can go to other places in the cell or to the membrane for secretion. Products are tagged chemically so they go to the correct places </li></ul>
  48. 51. Lysosome
  49. 52. lysosome <ul><li>Structure: </li></ul><ul><li>Membranous sac with digestive enzymes </li></ul><ul><li>Only in animal cells </li></ul><ul><li>Functions: </li></ul><ul><li>Intracellular digestion of macromolecules </li></ul><ul><li>Recycling of damaged organelles called autophagy </li></ul>
  50. 54. vacuoles <ul><li>Similar to lysosomes, have hydrolytic enzymes </li></ul><ul><li>Present in plant and fungi, protista </li></ul><ul><li>Functions: </li></ul><ul><li>Food vacuoles store food </li></ul><ul><li>Contractile vacuoles in protists control water and salts </li></ul><ul><li>Plants have central vacuole that gives internal support, stores organic molecules, pigments, poisons </li></ul>
  51. 56. mitochondria <ul><li>Structure: </li></ul><ul><li>Double membrane </li></ul><ul><li>Inner membrane has infolds called cristae </li></ul><ul><li>Inside is called matrix </li></ul><ul><li>Has its own DNA and ribosomes </li></ul><ul><li>Enzymes embedded into the membrane and in the matrix </li></ul><ul><li>Function: </li></ul><ul><li>Synthesis of ATP from glucose  cellular respiration </li></ul>
  52. 58. chloroplast <ul><li>Structure </li></ul><ul><li>Double membrane </li></ul><ul><li>Inner compartment made up of another intermebranous system called thylakoids </li></ul><ul><li>Fluid around the thylakoids called stroma </li></ul><ul><li>Function </li></ul><ul><li>Photosynthesis </li></ul>
  53. 59. Extracellular components <ul><li>Plant cells have a cell wall made of cellulose </li></ul><ul><li>Helps maintain shape, prevents water uptake </li></ul><ul><li>Animal cells secrete glycoproteins that function in support, adhesion and movement </li></ul>

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