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Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
Microscopes
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Microscopes

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  • 1. Microscopy: The Science of the Microscope
  • 2. The Invention of the Microscope • Renaissance invention (Mid 1600s) • Credit for invention goes to Anton Van Leeuenhoek • Constructed simple curved glass lenses in combination
  • 3. Improving the Microscope • Robert Hooke • English biologist who discovered cells • Increased magnification with improved lenses
  • 4. Modern Compound Light Microscopes • Uses 2 lenses in combination to magnify an image • Can view objects too small to be seen with unaided eye • Object must be thin enough for light to pass through • Can view living things • Typical magnification 100x to 1000x See page 17 of your packet for a detailed discussion of: • Parts and their functions • Proper use and handling • Procedures for making a wet mount
  • 5. Pushing the Limits: Electron Microscopes • A light microscope cannot be used to distinguish objects that are smaller than half the wavelength of light • Any object with a diameter smaller than 0.275 micrometers will be invisible or, at best, show up as a blur • Electrons are speeded up in a vacuum until their wavelength is extremely short, only one hundred-thousandth that of white light. • Electron microscopes were developed in the 1930s
  • 6. Electron Microscopes • Uses a beam of electron to view the specimen (not light) • Specimen viewed must be prepared in a vacuum (no air molecules) therefore living things cannot be viewed using this type of scope • Magnifies up to 200,000x magnification
  • 7. Scanning Electron Microscope
  • 8. Scanning Electron Microscope or SEM • Bounces electrons off the surface of the object • Produces a 3 dimensional image of the object
  • 9. Red Blood Cells
  • 10. Blood Clot
  • 11. Nerve Cells
  • 12. Tongue with a Taste Bud
  • 13. Sperm on Surface of Human Egg
  • 14. The Split End of a Human Hair
  • 15. Tooth Plaque
  • 16. Transmission Electron Microscope
  • 17. Transmission Electron Microscope • Electrons pass through the object forming a one dimensional picture • Allows one to view the inside of an object (ex. internal structure of a cell)
  • 18. filamentous bacteria from the gut of a termite
  • 19. Sperm heads from a stick insect
  • 20. Salmonella Bacteria
  • 21. Stereoscope • Allows viewing of macroscopic objects with great detail • Does not require light to pass through object • Can view living things • Typical magnification of 10X to 30X
  • 22. Choosing the Correct Microscope
  • 23. Microscope Lab Skills Review Complete the microscope review activities on pages 41 and 43-44.

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