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  • 1. Microscopy: The Science of the Microscope
  • 2. The Invention of the Microscope
    • Renaissance invention (Mid 1600s)
    • 3. Credit for invention goes to Anton Van Leeuenhoek
    • 4. Constructed simple curved glass lenses in combination
  • Improving the Microscope
    • Robert Hooke
    • 5. English biologist who discovered cells
    • 6. Increased magnification with improved lenses
  • Modern Compound Light Microscopes
    • Uses 2 lenses in combination to magnify an image
    • 7. Can view objects too small to be seen with unaided eye
    • 8. Object must be thin enough for light to pass through
    • 9. Can view living things
    • 10. Typical magnification 100x to 1000x
    See page 17 of your packet for a detailed discussion of:
    • Parts and their functions
    • 11. Proper use and handling
    • 12. Procedures for making a wet mount
  • Pushing the Limits: Electron Microscopes
    • A light microscope cannot be used to distinguish objects that are smaller than half the wavelength of light
    • 13. Any object with a diameter smaller than 0.275 micrometers will be invisible or, at best, show up as a blur
    • 14. Electrons are speeded up in a vacuum until their wavelength is extremely short, only one hundred-thousandth that of white light.
    • 15. Electron microscopes were developed in the 1930s
  • Electron Microscopes
    • Uses a beam of electron to view the specimen (not light)
    • 16. Specimen viewed must be prepared in a vacuum (no air molecules) therefore living things cannot be viewed using this type of scope
    • 17. Magnifies up to 200,000x magnification
  • Scanning Electron Microscope
  • 18. Scanning Electron Microscope or SEM
    Bounces electrons off the surface of the object
    Produces a 3 dimensional image of the object
  • 19. Red Blood Cells
  • 20. Blood Clot
  • 21. Nerve Cells
  • 22. Tongue with a Taste Bud
  • 23. Sperm on Surface of Human Egg
  • 24. The Split End of a Human Hair
  • 25. Tooth Plaque
  • 26. Transmission Electron Microscope
  • 27. Transmission Electron Microscope
    Electrons pass through the object forming a one dimensional picture
    Allows one to view the inside of an object (ex. internal structure of a cell)
  • 28. filamentous bacteria from the gut of a termite
  • 29. Sperm heads from a stick insect
  • 30. Salmonella Bacteria
  • 31. Stereoscope
    • Allows viewing of macroscopic objects with great detail
    • 32. Does not require light to pass through object
    • 33. Can view living things
    • 34. Typical magnification of 10X to 30X
  • Choosing the Correct Microscope
  • 35. Microscope Lab Skills Review
    Complete the microscope review activities on pages 41 and 43-44.