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Microscopes

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Microscopes Microscopes Presentation Transcript

  • Microscopy: The Science of the Microscope
  • The Invention of the Microscope
    • Renaissance invention (Mid 1600s)
    • Credit for invention goes to Anton Van Leeuenhoek
    • Constructed simple curved glass lenses in combination
  • Improving the Microscope
    • Robert Hooke
    • English biologist who discovered cells
    • Increased magnification with improved lenses
  • Modern Compound Light Microscopes
    • Uses 2 lenses in combination to magnify an image
    • Can view objects too small to be seen with unaided eye
    • Object must be thin enough for light to pass through
    • Can view living things
    • Typical magnification 100x to 1000x
    See page 17 of your packet for a detailed discussion of:
    • Parts and their functions
    • Proper use and handling
    • Procedures for making a wet mount
  • Pushing the Limits: Electron Microscopes
    • A light microscope cannot be used to distinguish objects that are smaller than half the wavelength of light
    • Any object with a diameter smaller than 0.275 micrometers will be invisible or, at best, show up as a blur
    • Electrons are speeded up in a vacuum until their wavelength is extremely short, only one hundred-thousandth that of white light.
    • Electron microscopes were developed in the 1930s
  • Electron Microscopes
    • Uses a beam of electron to view the specimen (not light)
    • Specimen viewed must be prepared in a vacuum (no air molecules) therefore living things cannot be viewed using this type of scope
    • Magnifies up to 200,000x magnification
  • Scanning Electron Microscope
  • Scanning Electron Microscope or SEM
    Bounces electrons off the surface of the object
    Produces a 3 dimensional image of the object
  • Red Blood Cells
  • Blood Clot
  • Nerve Cells
  • Tongue with a Taste Bud
  • Sperm on Surface of Human Egg
  • The Split End of a Human Hair
  • Tooth Plaque
  • Transmission Electron Microscope
  • Transmission Electron Microscope
    Electrons pass through the object forming a one dimensional picture
    Allows one to view the inside of an object (ex. internal structure of a cell)
  • filamentous bacteria from the gut of a termite
  • Sperm heads from a stick insect
  • Salmonella Bacteria
  • Stereoscope
    • Allows viewing of macroscopic objects with great detail
    • Does not require light to pass through object
    • Can view living things
    • Typical magnification of 10X to 30X
  • Choosing the Correct Microscope
  • Microscope Lab Skills Review
    Complete the microscope review activities on pages 41 and 43-44.