Your SlideShare is downloading. ×
Upcoming SlideShare
Loading in...5

Thanks for flagging this SlideShare!

Oops! An error has occurred.

Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply



Published on

Published in: Education, Technology
  • Be the first to comment

No Downloads
Total Views
On Slideshare
From Embeds
Number of Embeds
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

No notes for slide


  • 1. Microscopy: The Science of the Microscope
  • 2. The Invention of the Microscope • Renaissance invention (Mid 1600s) • Credit for invention goes to Anton Van Leeuenhoek • Constructed simple curved glass lenses in combination
  • 3. Improving the Microscope • Robert Hooke • English biologist who discovered cells • Increased magnification with improved lenses
  • 4. Modern Compound Light Microscopes • Uses 2 lenses in combination to magnify an image • Can view objects too small to be seen with unaided eye • Object must be thin enough for light to pass through • Can view living things • Typical magnification 100x to 1000x See page 17 of your packet for a detailed discussion of: • Parts and their functions • Proper use and handling • Procedures for making a wet mount
  • 5. Pushing the Limits: Electron Microscopes • A light microscope cannot be used to distinguish objects that are smaller than half the wavelength of light • Any object with a diameter smaller than 0.275 micrometers will be invisible or, at best, show up as a blur • Electrons are speeded up in a vacuum until their wavelength is extremely short, only one hundred-thousandth that of white light. • Electron microscopes were developed in the 1930s
  • 6. Electron Microscopes • Uses a beam of electron to view the specimen (not light) • Specimen viewed must be prepared in a vacuum (no air molecules) therefore living things cannot be viewed using this type of scope • Magnifies up to 200,000x magnification
  • 7. Scanning Electron Microscope
  • 8. Scanning Electron Microscope or SEM • Bounces electrons off the surface of the object • Produces a 3 dimensional image of the object
  • 9. Red Blood Cells
  • 10. Blood Clot
  • 11. Nerve Cells
  • 12. Tongue with a Taste Bud
  • 13. Sperm on Surface of Human Egg
  • 14. The Split End of a Human Hair
  • 15. Tooth Plaque
  • 16. Transmission Electron Microscope
  • 17. Transmission Electron Microscope • Electrons pass through the object forming a one dimensional picture • Allows one to view the inside of an object (ex. internal structure of a cell)
  • 18. filamentous bacteria from the gut of a termite
  • 19. Sperm heads from a stick insect
  • 20. Salmonella Bacteria
  • 21. Stereoscope • Allows viewing of macroscopic objects with great detail • Does not require light to pass through object • Can view living things • Typical magnification of 10X to 30X
  • 22. Choosing the Correct Microscope
  • 23. Microscope Lab Skills Review Complete the microscope review activities on pages 41 and 43-44.