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Products: Adipose-Derived Stem Cells

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Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into chondrocytes, ...

Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into chondrocytes, osteoblasts and adipocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1, and then cryopreserved. Post-thaw viable cells are tested for proliferation and differentiation capacity into adipocytes, osteocytes and chondrocytes.

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    Products: Adipose-Derived Stem Cells Products: Adipose-Derived Stem Cells Document Transcript

    •   Adipose-Derived Stem Cells (ADSCs)   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Bovine Adipose-Derived Stem Cells (bADSCs) PRODUCT NAME PRODUCT SIZE CATALOG NUMBER Bovine ADSCs 1 vial, 106 cells, cryopreserved Bovine ADSCs 1 vial, 106 cells, proliferating BAD001C Bovine ADSCs 1 vial, 106 cells, pellet in RNA later BAD001P Bovine ADSCs 4 µg total RNA BAD001R Bovine ADSCs cDNA for 10 PCR rxns BAD001D BAD001F Description Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into adipocytes, chondrocytes, and osteocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1 (Fig. 1), and then cryopreserved. Postthaw viable cells are tested for proliferation and Figure  1.  Bovine  adipose-­‐ derived  stem  cells  in  culture   at  passage  1. differentiation capacity into chondrocytes, osteocytes, and adipocytes (Fig. 2).   Figure  2.  Analysis  of  differentiation  to  chondrogenic  (Alcian  Blue),  osteogenic  (Von  Kossa),   and  adipogenic  (Oil  Red  O)  lineages  of  bovine  adipose-­‐derived  stem  cells.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Specifications Cell Type: Age: Specie: Tissue: Form: Viability: Test performed: Shipping Condition: Storage: adipose-derived stem cells juvenile bovine adipose tissue from subcutaneous fat cryopreserved >70% negative for mycoplasma, bacteria, and fungi dry ice liquid nitrogen temperature Recommended thaw/culture protocol for cryopreserved Bovine Adipose-Derived Stem Cells (BAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous. Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid any possible contamination. Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood. 1. Once the product is delivered, keep it in liquid nitrogen for long storage. 2. If you want to use cells immediately, pre-warm media and wipe the cryovial with 70% ethanol prior to introduce in a laminar flow hood. Then, open the cap carefully to remove the excess of pressure and then close it again. 3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2 minutes. 4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL polypropylene sterile tube with 5-10 mL of pre-warmed media. We recommend using α-MEM or DMEM-low glucose supplemented with 10% (v/v) FBS and 1% (v/v) antibiotics. 5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to eliminate DMSO traces. 6. Discard supernatant and resuspend the pellet in complete medium.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   7. Count cells to see cell viability by Trypan Blue staining or similar methodology. Bovine adipose-derived stem cells (BAD001F) contain at least 1 x 106 viable cells. 8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and 5% CO2. Recommended initial cell seeding is 10,000 cells/cm2. 9. To remove dead cells, replace media after 24-48 hours of plating and since then, every 2-3 days. 10. When cells reached 80-90% of confluence, harvest cells using trypsin or similar technique to detach them from the flask. We recommend not using these cells for experiments and waiting until next passage. 11. Culture into a new flask for expansion at a cell seeding density of 2,000 cells/cm2. 12. Repeat steps 10 and 11 until you use them for your experiments. After 10-15 passages, cells can reduce their proliferation rate. For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic, therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Canine Adipose-Derived Stem Cells (cADSCs) PRODUCT NAME PRODUCT SIZE CATALOG NUMBER Canine ADSCs 1 vial, 106 cells, cryopreserved Canine ADSCs 1 vial, 106 cells, proliferating CAD001C Canine ADSCs 1 vial, 106 cells, pellet in RNA later CAD001P Canine ADSCs 4 µg total RNA CAD001R Canine ADSCs cDNA for 10 PCR rxns CAD001D CAD001F Description Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into adipocytes, chondrocytes, and osteocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1 (Fig. 1), and then cryopreserved. Post-thaw viable cells are tested for proliferation and differentiation Figure  3.  Canine  adipose-­‐ derived  stem  cells  in  culture   at  passage  1. capacity into chondrocytes, osteocytes, and adipocytes (Fig. 2).   Figure  4.  Analysis  of  differentiation  to  chondrogenic  (Alcian  Blue),  osteogenic  (Von  Kossa),   and  adipogenic  (Oil  Red  O)  lineages  of  canine  adipose-­‐derived  stem  cells.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Specifications Cell Type: Age: Specie: Tissue: Form: Viability: Test performed: Shipping Condition: Storage: adipose-derived stem cells adult canine adipose tissue from subcutaneous fat cryopreserved >70% negative for mycoplasma, bacteria, and fungi dry ice liquid nitrogen temperature Recommended thaw/culture protocol for cryopreserved Canine Adipose-Derived Stem Cells (CAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous. Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid any possible contamination. Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood. 1. Once the product is delivered, keep it in liquid nitrogen for long storage. 2. If you want to use cells immediately, pre-warm media and wipe the cryovial with 70% ethanol prior to introduce in a laminar flow hood. Then, open the cap carefully to remove the excess of pressure and then close it again. 3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2 minutes. 4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL polypropylene sterile tube with 5-10 mL of pre-warmed media. We recommend using α-MEM or DMEM-low glucose supplemented with 10% (v/v) FBS and 1% (v/v) antibiotics. 5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to eliminate DMSO traces. 6. Discard supernatant and resuspend the pellet in complete medium.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   7. Count cells to see cell viability by Trypan Blue staining or similar methodology. Canine adipose-derived stem cells (CAD001F) contain at least 1 x 106 viable cells. 8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and 5% CO2. Recommended initial cell seeding is 10,000 cells/cm2. 9. To remove dead cells, replace media after 24-48 hours of plating and since then, every 2-3 days. 10. When cells reached 80-90% of confluence, harvest cells using trypsin or similar technique to detach them from the flask. We recommend not using these cells for experiments and waiting until next passage. 11. Culture into a new flask for expansion at a cell seeding density of 2,000 cells/cm2. 12. Repeat steps 10 and 11 until you use them for your experiments. After 10-15 passages, cells can reduce their proliferation rate. For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic, therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Equine Adipose-Derived Stem Cells (eADSCs) PRODUCT NAME PRODUCT SIZE CATALOG NUMBER Equine ADSCs 1 vial, 106 cells, cryopreserved Equine ADSCs 1 vial, 106 cells, proliferating EAD001C Equine ADSCs 1 vial, 106 cells, pellet in RNA later EAD001P Equine ADSCs 4 µg total RNA EAD001R Equine ADSCs cDNA for 10 PCR rxns EAD001D EAD001F Description Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into adipocytes, chondrocytes, and osteocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1 (Fig. 1), and then cryopreserved. Post-thaw viable cells are tested for proliferation and differentiation Figure  5.  Equine  adipose-­‐ derived  stem  cells  in  culture   at  passage  1. capacity into chondrocytes, osteocytes, and adipocytes (Fig. 2).   Figure  6.  Analysis  of  differentiation  to  chondrogenic  (Alcian  Blue),  osteogenic  (Von  Kossa),   and  adipogenic  (Oil  Red  O)  lineages  of  equine  adipose-­‐derived  stem  cells.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Specifications Cell Type: Age: Specie: Tissue: Form: Viability: Test performed: Shipping Condition: Storage: adipose-derived stem cells juvenile equine adipose tissue from subcutaneous fat cryopreserved >70% negative for mycoplasma, bacteria, and fungi dry ice liquid nitrogen temperature Recommended thaw/culture protocol for cryopreserved Equine Adipose-Derived Stem Cells (EAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous. Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid any possible contamination. Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood. 1. Once the product is delivered, keep it in liquid nitrogen for long storage. 2. If you want to use cells immediately, pre-warm media and wipe the cryovial with 70% ethanol prior to introduce in a laminar flow hood. Then, open the cap carefully to remove the excess of pressure and then close it again. 3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2 minutes. 4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL polypropylene sterile tube with 5-10 mL of pre-warmed media. We recommend using α-MEM or DMEM-low glucose supplemented with 10% (v/v) FBS and 1% (v/v) antibiotics. 5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to eliminate DMSO traces. 6. Discard supernatant and resuspend the pellet in complete medium.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   7. Count cells to see cell viability by Trypan Blue staining or similar methodology. Equine adipose-derived stem cells (EAD001F) contain at least 1 x 106 viable cells. 8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and 5% CO2. Recommended initial cell seeding is 10,000 cells/cm2. 9. To remove dead cells, replace media after 24-48 hours of plating and since then, every 2-3 days. 10. When cells reached 80-90% of confluence, harvest cells using trypsin or similar technique to detach them from the flask. We recommend not using these cells for experiments and waiting until next passage. 11. Culture into a new flask for expansion at a cell seeding density of 2,000 cells/cm2. 12. Repeat steps 10 and 11 until you use them for your experiments. After 10-15 passages, cells can reduce their proliferation rate. For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic, therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Feline Adipose-Derived Stem Cells (fADSCs) PRODUCT NAME PRODUCT SIZE CATALOG NUMBER Feline ADSCs 1 vial, 106 cells, cryopreserved Feline ADSCs 1 vial, 106 cells, proliferating FAD001C Feline ADSCs 1 vial, 106 cells, pellet in RNA later FAD001P Feline ADSCs 4 µg total RNA FAD001R Feline ADSCs cDNA for 10 PCR rxns FAD001D FAD001F Description Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into adipocytes, chondrocytes, and osteocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1 (Fig. 1), and then cryopreserved. Post-thaw viable cells are tested for proliferation and differentiation Figure  7.  Feline  adipose-­‐ derived  stem  cells  in  culture   at  passage  1. capacity into chondrocytes, osteocytes, and adipocytes (Fig. 2).   Figure  8.  Analysis  of  differentiation  to  chondrogenic  (Alcian  Blue),  osteogenic  (Von  Kossa),   and  adipogenic  (Oil  Red  O)  lineages  of  feline  adipose-­‐derived  stem  cells.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Specifications Cell Type: Age: Specie: Tissue: Form: Viability: Test performed: Shipping Condition: Storage: adipose-derived stem cells adult feline adipose tissue from subcutaneous fat cryopreserved >70% negative for mycoplasma, bacteria, and fungi dry ice liquid nitrogen temperature Recommended thaw/culture protocol for cryopreserved Feline Adipose-Derived Stem Cells (FAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous. Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid any possible contamination. Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood. 1. Once the product is delivered, keep it in liquid nitrogen for long storage. 2. If you want to use cells immediately, pre-warm media and wipe the cryovial with 70% ethanol prior to introduce in a laminar flow hood. Then, open the cap carefully to remove the excess of pressure and then close it again. 3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2 minutes. 4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL polypropylene sterile tube with 5-10 mL of pre-warmed media. We recommend using α-MEM or DMEM-low glucose supplemented with 10% (v/v) FBS and 1% (v/v) antibiotics. 5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to eliminate DMSO traces. 6. Discard supernatant and resuspend the pellet in complete medium.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   7. Count cells to see cell viability by Trypan Blue staining or similar methodology. Feline adipose-derived stem cells (FAD001F) contain at least 1 x 106 viable cells. 8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and 5% CO2. Recommended initial cell seeding is 10,000 cells/cm2. 9. To remove dead cells, replace media after 24-48 hours of plating and since then, every 2-3 days. 10. When cells reached 80-90% of confluence, harvest cells using trypsin or similar technique to detach them from the flask. We recommend not using these cells for experiments and waiting until next passage. 11. Culture into a new flask for expansion at a cell seeding density of 2,000 cells/cm2. 12. Repeat steps 10 and 11 until you use them for your experiments. After 10-15 passages, cells can reduce their proliferation rate. For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic, therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Ovine Adipose-Derived Stem Cells (oADSCs) PRODUCT NAME PRODUCT SIZE CATALOG NUMBER Ovine ADSCs 1 vial, 106 cells, cryopreserved Ovine ADSCs 1 vial, 106 cells, proliferating OAD001C Ovine ADSCs 1 vial, 106 cells, pellet in RNA later OAD001P Ovine ADSCs 4 µg total RNA OAD001R Ovine ADSCs cDNA for 10 PCR rxns OAD001D OAD001F Description Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into adipocytes, chondrocytes, and osteocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1 (Fig. 1), and then cryopreserved. Post-thaw viable cells are tested for proliferation and differentiation Figure  9.  Ovine  adipose-­‐ derived  stem  cells  in  culture   at  passage  1. capacity into chondrocytes, osteocytes, and adipocytes (Fig. 2).   Figure  10.  Analysis  of  differentiation  to  chondrogenic  (Alcian  Blue),  osteogenic  (Von   Kossa),  and  adipogenic  (Oil  Red  O)  lineages  of  ovine  adipose-­‐derived  stem  cells.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Specifications Cell Type: Age: Specie: Tissue: Form: Viability: Test performed: Shipping Condition: Storage: adipose-derived stem cells juvenile ovine adipose tissue from subcutaneous fat cryopreserved >70% negative for mycoplasma, bacteria, and fungi dry ice liquid nitrogen temperature Recommended thaw/culture protocol for cryopreserved Ovine Adipose-Derived Stem Cells (OAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous. Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid any possible contamination. Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood. 1. Once the product is delivered, keep it in liquid nitrogen for long storage. 2. If you want to use cells immediately, pre-warm media and wipe the cryovial with 70% ethanol prior to introduce in a laminar flow hood. Then, open the cap carefully to remove the excess of pressure and then close it again. 3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2 minutes. 4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL polypropylene sterile tube with 5-10 mL of pre-warmed media. We recommend using α-MEM or DMEM-low glucose supplemented with 10% (v/v) FBS and 1% (v/v) antibiotics. 5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to eliminate DMSO traces. 6. Discard supernatant and resuspend the pellet in complete medium.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   7. Count cells to see cell viability by Trypan Blue staining or similar methodology. Ovine adipose-derived stem cells (OAD001F) contain at least 1 x 106 viable cells. 8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and 5% CO2. Recommended initial cell seeding is 10,000 cells/cm2. 9. To remove dead cells, replace media after 24-48 hours of plating and since then, every 2-3 days. 10. When cells reached 80-90% of confluence, harvest cells using trypsin or similar technique to detach them from the flask. We recommend not using these cells for experiments and waiting until next passage. 11. Culture into a new flask for expansion at a cell seeding density of 2,000 cells/cm2. 12. Repeat steps 10 and 11 until you use them for your experiments. After 10-15 passages, cells can reduce their proliferation rate. For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic, therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Swine Adipose-Derived Stem Cells (sADSCs) PRODUCT NAME PRODUCT SIZE CATALOG NUMBER Swine ADSCs 1 vial, 106 cells, cryopreserved SAD001F Swine ADSCs 6 1 vial, 10 cells, proliferating 6 SAD001C Swine ADSCs 1 vial, 10 cells, pellet in RNA later SAD001P Swine ADSCs 4 µg total RNA SAD001R Swine ADSCs cDNA for 10 PCR rxns SAD001D Description Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into adipocytes, chondrocytes, and osteocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1 (Fig. 1), and then cryopreserved. Postthaw viable cells are tested for proliferation and Figure  11.  Swine  adipose-­‐ derived  stem  cells  in  culture   at  passage  1. differentiation capacity into chondrocytes, osteocytes, and adipocytes (Fig. 2).   Figure  12.  Analysis  of  differentiation  to  chondrogenic  (Alcian  Blue),  osteogenic  (Von   Kossa),  and  adipogenic  (Oil  Red  O)  lineages  of  swine  adipose-­‐derived  stem  cells.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   Specifications Cell Type: Age: Specie: Tissue: Form: Viability: Test performed: Shipping Condition: Storage: adipose-derived stem cells juvenile swine adipose tissue from subcutaneous fat cryopreserved >70% negative for mycoplasma, bacteria, and fungi dry ice liquid nitrogen temperature Recommended thaw/culture protocol for cryopreserved Swine Adipose-Derived Stem Cells (SAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous. Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid any possible contamination. Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood. 1. Once the product is delivered, keep it in liquid nitrogen for long storage. 2. If you want to use cells immediately, pre-warm media and wipe the cryovial with 70% ethanol prior to introduce in a laminar flow hood. Then, open the cap carefully to remove the excess of pressure and then close it again. 3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2 minutes. 4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL polypropylene sterile tube with 5-10 mL of pre-warmed media. We recommend using α-MEM or DMEM-low glucose supplemented with 10% (v/v) FBS and 1% (v/v) antibiotics. 5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to eliminate DMSO traces. 6. Discard supernatant and resuspend the pellet in complete medium.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com
    •   7. Count cells to see cell viability by Trypan Blue staining or similar methodology. Swine adipose-derived stem cells (SAD001F) contain at least 1 x 106 viable cells. 8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and 5% CO2. Recommended initial cell seeding is 10,000 cells/cm2. 9. To remove dead cells, replace media after 24-48 hours of plating and since then, every 2-3 days. 10. When cells reached 80-90% of confluence, harvest cells using trypsin or similar technique to detach them from the flask. We recommend not using these cells for experiments and waiting until next passage. 11. Culture into a new flask for expansion at a cell seeding density of 2,000 cells/cm2. 12. Repeat steps 10 and 11 until you use them for your experiments. After 10-15 passages, cells can reduce their proliferation rate. For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic, therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.   www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. info@cellider.com