Malformation of thephysicalstructureisbecauseitdidn’tdevelopedcompletelywhileembryonicdevelopment. Lack of embryonicdevelopment: Low blood flow CongenicCitomegalovirus (cCMV) Genetic mutation (LIS1, XLIS)
Researches Malformations of cortical development in children with congenital cytomegalovirus infection - A study of nine children with proven congenital cytomegalovirus infection Development of Lissencephaly in the fetus as direct consequence of early infection. Most common vertically transmitted disease rate of the infection ranging: 0.2 to 2.4% in newborn infants 40.000 infants per year
Chemical remedy Oral Hexadecyloxypropyl-Cidofovir Therapy in Pregnant Guinea Pigs Improves Outcome in the Congenital Model of Cytomegalovirus Infection Alternative orally supply analog of the existing drug, Cidofovir Only proved effiency of Guinea Pigs
Why supply antibodies?
Hypothesis The augment of antibodies IgG and IgM may reduce the presence of CVM, representing a minor risk to the fetus in developing Lissencephaly.
Develop a treatment with antibodies against the growth of Citomegalovirus that causes Lyssencephaly
Reduce the CMV presence in the placenta
Lower the risks of developing Lissencephaly caused by cCMV.
Prepare research models Mice from 12-13 days Anesthiaze mice Inject with CMV vector solution with a glass micropipette Place pipette for an 30 s and then slowly withdrawn.
Research model’s distribution Groups: IL 6 IL 28 IgG IgM Sub-groups: A= every week B= every 48 hrs C= every three days
Step: Viral presence assays
Extract serology: CVS Placenta is the common path Chronic Villous Sampling Suction of a tissue from placenta
Step: ELISPOT Quantify presence of IgG and IgM in all experimental groups Procedure according to: “General ELISPOT procedure” by abcam.com
Step: Direct immunofluorescence Cualitative presence of IgG and IgM in all experimental groups Process according to: “Immunostaining of cells in tissue culture” by ENCORE biotechnology INC.
Spected results ELISPOT will indicate: augment in antibodies in experimental groups will mean lower presence of CMV Direct immunofluorescence: Control group will be brighter than experimental groups will mean high presence of CMV
Bibliography Bosnjak VM, Daković I, et al. Malformations of cortical development in children with congenital cytomegalovirus infection - A study of nine children with proven congenital cytomegalovirus infection. January, 2011. http://www.ncbi.nlm.nih.gov/pubmed/21648339 Ravo FJ, Bernstein DI, et al. Oral Hexadecyloxypropyl-Cidofovir Therapy in Pregnant Guinea Pigs Improves Outcome in the Congenital Model of Cytomegalovirus Infection. November 15, 2011. http://www.ncbi.nlm.nih.gov/pubmed?term=Oral%20Hexadecyloxypropyl-Cidofovir%20Therapy%20in%20Pregnant%20Guinea%20Pigs%20Improves%20Outcome%20in%20the%20Congenital%20Model%20of%20Cytomegalovirus%20Infection%20
Araswathy TS, Az-Ulhusna A, et al. Seroprevalence of cytomegalovirus infection in pregnant women and associated role in obstetric complications: a preliminary study. March, 2011. http://www.ncbi.nlm.nih.gov/pubmed/21710852 Kaneda K, Kasahara H, et al.Selective Optical Control of Synaptic Transmission in the Subcortical Visual Pathway by Activation of Viral Vector-Expressed Halorhodopsin. April 5, 2011. http://www.ncbi.nlm.nih.gov/pubmed?term=Selective%20Optical%20Control%20of%20Synaptic%20Transmission%20in%20the%20Subcortical%20Visual%20Pathway%20by%20Activation%20of%20Viral%20Vector-Expressed%20Halorhodopsin Boeckh, M. and Boivin, G. Quantitation of Cytomegalovirus: Methodologic Aspects and Clinical Applications. Clinical Microbiology Reviews, July 1998, p. 533-554, Vol. 11, No. 3. http://cmr.asm.org/cgi/content/full/11/3/533