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7.3 Transcription
Topic 7 Nucleic Acids & Proteins
Transcription
 7.3.1 State that transcription is carried out in a 5’→ 3’
direction. (The 5’ end of the free RNA nucleotid...
Transcription
 7.3.3 Explain the process of transcription in prokaryotes,
including the role of the promoter region, RNA ...
Transcription
 7.3.4 State that eukaryotic RNA needs the removal of
introns to form mature mRNA.
 Further details of the...
Transcription
 Transcription is the formation of a molecule of messenger
RNA (mRNA) from a DNA template in the nucleus.
...
Transcription
Ref: Biology for the IB Diploma, Allott
Transcription
Ref: IB Biology, Oxford Study Courses
Transcription
 The Promoter Region
 A specific sequence of DNA bases at the start of the gene to
which RNA polymerase bi...
Role of the Promoter and Terminator in
Transcription
Ref: Biology for the IB Diploma, Allott
Introns and Exons
 Many genes in Eukaryotes contain Introns.
 These are non-coding sequences that are transcribed but
no...
Introns and Exons
Ref: IB Biology, Oxford Study Courses
Transcription
 7.3.1 State that transcription is carried out in a 5’→ 3’
direction. (The 5’ end of the free RNA nucleotid...
Transcription
 7.3.3 Explain the process of transcription in prokaryotes,
including the role of the promoter region, RNA ...
Transcription
 7.3.4 State that eukaryotic RNA needs the removal of
introns to form mature mRNA.
 Further details of the...
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7.3 transcription

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Transcript of "7.3 transcription"

  1. 1. 7.3 Transcription Topic 7 Nucleic Acids & Proteins
  2. 2. Transcription  7.3.1 State that transcription is carried out in a 5’→ 3’ direction. (The 5’ end of the free RNA nucleotide is added to the 3’ end of the RNA molecule that is already synthesized).  7.3.2 Distinguish between the sense and antisense strands of DNA.  The sense strand (coding strand) has the same base sequence as mRNA with uracil instead of thymine. The antisense (template) strand is transcribed.
  3. 3. Transcription  7.3.3 Explain the process of transcription in prokaryotes, including the role of the promoter region, RNA polymerase, nucleoside triphosphates and the terminator.  The following details are not required: there is more than one type of RNA polymerase; features of the promoter region; the need for transcription protein factors for RNA polymerase binding; TATA boxes (and other repetitive sequences); and the exact sequence of the bases that act as terminators.
  4. 4. Transcription  7.3.4 State that eukaryotic RNA needs the removal of introns to form mature mRNA.  Further details of the process of post-transcriptional modification of RNA are not required.
  5. 5. Transcription  Transcription is the formation of a molecule of messenger RNA (mRNA) from a DNA template in the nucleus.  Transcription is carried out in a 5’  3’ direction.  DNA acts as a template but only one side of the DNA double helix is a gene. This is called the antisense strand.  The antisense strand is transcribed into mRNA by RNA polymerase.  The other side is complementary to the antisense strand and is called the sense strand.  The sense strand has the same sequence of bases as the mRNA except with T not U.
  6. 6. Transcription Ref: Biology for the IB Diploma, Allott
  7. 7. Transcription Ref: IB Biology, Oxford Study Courses
  8. 8. Transcription  The Promoter Region  A specific sequence of DNA bases at the start of the gene to which RNA polymerase binds.  It is found on the sense strand.  RNA Polymerase  The enzyme adds nucleoside triphosphates (NTPs) using base pairing to the DNA antisense strand.  Terminator Region  A specific sequence of DNA bases marking the end of the transcription process. RNA polymerase breaks free and the mRNA strand is released. It is found on the sense strand.
  9. 9. Role of the Promoter and Terminator in Transcription Ref: Biology for the IB Diploma, Allott
  10. 10. Introns and Exons  Many genes in Eukaryotes contain Introns.  These are non-coding sequences that are transcribed but not translated.  The are found in newly transcribed mRNA but removed to form mature mRNA.  The sequences that are not removed are called Exons.  Prokaryotes do not usually have introns.  The removal of introns is called post-transcriptional modification of RNA.
  11. 11. Introns and Exons Ref: IB Biology, Oxford Study Courses
  12. 12. Transcription  7.3.1 State that transcription is carried out in a 5’→ 3’ direction. (The 5’ end of the free RNA nucleotide is added to the 3’ end of the RNA molecule that is already synthesized).  7.3.2 Distinguish between the sense and antisense strands of DNA.  The sense strand (coding strand) has the same base sequence as mRNA with uracil instead of thymine. The antisense (template) strand is transcribed.
  13. 13. Transcription  7.3.3 Explain the process of transcription in prokaryotes, including the role of the promoter region, RNA polymerase, nucleoside triphosphates and the terminator.  The following details are not required: there is more than one type of RNA polymerase; features of the promoter region; the need for transcription protein factors for RNA polymerase binding; TATA boxes (and other repetitive sequences); and the exact sequence of the bases that act as terminators.
  14. 14. Transcription  7.3.4 State that eukaryotic RNA needs the removal of introns to form mature mRNA.  Further details of the process of post-transcriptional modification of RNA are not required.
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