The efficiency of extracts of plants that have

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  • 1. EFFICIENCY OF PLANTS EXTRACTS THAT CONTAIN MELATONIN IN PARKINSON DISEASE RATS Carlos J. Santos Pérez, RISE Student
  • 2. Background Information  The rats have a very similar neurological system to human.  The cause of Parkinson Disease in a patient is the absence of dopamine.  Symptoms:  Trembling of the extremities  Stiffness  Slowness  Poor balance or coordination
  • 3. Melatonin  Is an hormone that is produce in the brain by the pineal gland, from the amino acid tryptophan.  Synthesis and release of melatonin are stimulated by darkness and suppressed by light, suggesting the involvement of melatonin in circadian rhythm and regulation of diverse body functions.  Levels of melatonin in the blood are highest prior to bedtime.
  • 4. Melatonin Extract: Achillea millefolium  Achillea millefolium- a plant that is commonly called Achillea, we can found it in North America and it contains a high concentration of melatonin  45.4 ng/g of the plant.
  • 5. Melatonin & Alzheimer  Recent studies indicate that the presence of melatonin in Alzheimer improve patient health, so we can investigate if melatonin has the same effect in patients with other neurodegenerative disease , in this case Parkinson’s Disease.
  • 6. Objectives  The objectives of this investigation are:  Determine if the extract of Achillea help patience with Parkinson Disease to control their involuntarily movements.  Prove that melatonin is a diverse remedial hormone that can be use to treat Parkinson Disease Symptoms including the lack of sleeping symptom
  • 7. Hypothesis  If we apply melatonin extract of Achillea plant in rats with Parkinson Disease then we should see less involuntarily movement in the rats at higher concentrations of extract.
  • 8. Rotenone  The Rotenone Molecule is a mithocondrial inhibitor that have been used to induce dopaminergic neuronal death.  Inhibit the pathway of dopamine production.  That’s why it cause Parkinson’s Disease.  Rotenone at 3 mg/kg/day will induce degeneration of the nigrostriatal dopaminergic pathway (Caboni, 2004)  This will be subcutaneously
  • 9. Procedure  Extracts of Achillea millefolium.  Need a Population of mice for the experiment.  Groups:  Control- It consist of 25 healthy rats and 25 sick rats. They will not be affected by the extracts of Achillea millefolium.  Experimental- It consist of three group:  Concentration of 60%, will be injected subcutaneously.(25 rats)  Concentration of 10% , will be injected subcutaneously(25 rats)
  • 10. Rats Control Group Experimental Group Rats with Rats with Parkinson’s- 40% Rats with Parkinson’s Healthy Rat(No Parkinon’s concentration of 10% of ML(5mL ML) Disease(No Ml) ML(5mL subcutaneously) subcutaneously) Alcohol(25) Levodopa(25)
  • 11. How to do the extracts?  Tincture- Alcoholic extract that alcohol is the solvent.  We will use: 40% and 90% of alcohol and plant.  The ethanol extract have to be placed in rotatory evaporator to eliminate the solvent, obtaining an extract, which was weighed and stored in dissecator.
  • 12. Variables  Time of Dosis of both of the concentrations: daily 5ml/day This will be for at least 1 year.  Independent Variables- Concentrations and dosis of the extracts may vary.  Dependent Variable- the temblors and involuntary movements of the rats.  Type of Data- Qualitative. This research is based on the observation of the rats behaviors.
  • 13. Goals  If my hypothesis is reaffirmed I will proceed to use different plants to do the extracts and compare results.  As a second phase for my research project I will expand my research work to primates.
  • 14. References  Ferri, Fred F. Practical guide to the care of the medical patient. – 7th ed. Pages: 776-777  Marsden CD. Problems with long-term levodopa therapy for Parkinson's disease. Clin Neuropharmacol. 1994;Page 17  Marioni F. A straightforward procedure to biosynthesise melatonin using freshly chopped Achillea millefolium L. as reagent Phytochemistry Letters, Volume 1, Issue 2, 21 August 2008, Pages 107-110  German DC, Manaye K, Smith WK, Woodward DJ, Saper CB. Midbrain dopaminergic cell loss in Parkinson's disease: Computer visualization. Ann Neurol. 1989; 26:507–514.  http://www.ncbi.nlm.nih.gov/pubmed/18289173 Last visit- 7/25/2010  http://www.ncbi.nlm.nih.gov/pubmed/18289173 Last visit- 7/25/2010  http://www.ncbi.nlm.nih.gov/pubmed/15540952 Last visit- 7/25/2010  http://www.ncbi.nlm.nih.gov/pubmed/15540952 Last visit- 7/25/2010