Southern blot power point
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  • 1. SOUTHERN BLOTCAROLYN EYRE
  • 2. WHAT IS SOUTHERN BLOTTING? A process used for DNA analysis Developed by Edwin Southern in 1975 Is also referred to as DNA blotting Edwin Southern
  • 3. USE IN GENETICALLY MODIFIED ORGANISMS In genetically modified organisms, Southern blots are used to make certain that the necessary section of DNA has been inserted into the genome of the organism being modified.
  • 4. STEP 1 DNA is separated from it’s source and cut with a restriction enzyme.
  • 5. STEP 2 The fragments are put into a gel and are sorted by electrophoresis in order of length, the shortest at the top and the longest at the bottom.
  • 6. STEP 3 Ethidium bromide is then used to make the fragments visible, and the DNA is taken from the gel and placed in a nylon filter.
  • 7. STEP 4 Then a radioactive probe is added, which binds to complementary DNA fragments.
  • 8. STEP 5 The nylon membrane is then covered with an X- ray film which, when developed, reveals the position of the radioactive probe.