Supervisors: Prof. Dr. Ahmed Mohamed ZaghlolDarwish Professor of Cardiology Faculty of Medicine Tanta University Prof. Dr. Magdy Mohamed El-Masry Professor of Cardiology Faculty of Medicine Tanta University Prof. Dr. SamiaMahmoudSharf El-deen Professor of Cardiology Faculty of Medicine Tanta University
Hematopoietic stem cells Sources : Umbilical cord blood (1 per 1 million nucleated cell). Adult bone marrow (1 per 3 million nucleated cell). Mobilized peripheral blood (1 per 6 million nucleated cell).
Capable of unlimited cell proliferation in bone marrow producing mature blood cells.
Mesenchymal (stromal) stem cells: Present as a rare population of cells in bone marrow (0.001% to 0.01% of the nucleated cells). Other sources of MSC including placenta, adipose tissue, cord blood and liver. They can be cloned and expanded in vitro more than 1 million-fold and retain the ability to differentiate to several cells of mesodermal origin.
Endothelial progenitor cells. Cells can give rise to endothelial cells and contribute to endothelial recovery and new capillary formation after ischemia. Sources of endothelial progenitorcells: Bone marrow-derived EPCs. Non Bone marrow-derived EPCs: Which arise from Various tissues, including adipose tissue, neuronal tissue, and the heart itself.
Umbilical Cord Blood Cells. Perfect source for cell-based therapy as they are rich in both stem and progenitor cells. No ethical limitations regarding its use. No risks to the mother or to the newborn in collecting the cells after labor. There are many public and private UCB banks worldwide with cells readily available for transplantation at the most optimal time.
Adipose-derived stem cells. Recent studies have suggested that stem cells are present within the adipose tissue. This Adipose-derived stem cells, like MSCs, have the ability to differentiate toward the osteogenic, adipogenic, myogenic, chondrogenic and neurogenic cells. The ease of access to fat and its abundance makes adipose tissue a potentially useful source of stem cells for clinical applications.
Skeletal myoblasts. Skeletal myoblasts were the first candidates for cardiac repair. They are derived from skeletal muscles “satellite cells”. They are responsible for regeneration of damaged skeletal muscle after injury.