Algae Analytics and Nutraceutical Applications for Algae<br />Rhykka Connelly<br />r.connelly@cem.utexas.edu<br />April 27...
Standard Measurement Technologies<br />Nile Red is the current measurement tool of choice in the algae industry<br />Here’...
UT/OpenAlgae Technologies<br />Evaluating the intermediate products requires many analytical tools<br />
UT/OpenAlgae Technologies – Thin Layer Chromatography<br />Samples are taken before and after each processing step. Each s...
UT/OpenAlgae Technologies – Thin Layer Chromatography<br />Lipids released into the supernatant<br />Lipids remaining with...
 Suggests that we’re recovering beneficial lipids from sources other than triglycerides…possibly membrane lipids</li></li>...
UT/OpenAlgae Technologies – GC/MS<br />We can also track specific fatty acids, such as Omega 3-6-7-9, throughout growth an...
UT/OpenAlgae Technologies – Analysis Conclusions<br /><ul><li> The algae industry has relied on crude quantitative technol...
 UT sets itself apart by incorporating cutting edge technologies that measure lipids individually
 Adoption of these technologies will help increase understanding of algal lipid dynamics and standardize the way lipids ar...
 Certain kinds of algae make products that we’re interested in
 oil for fuel
 high in proteins and/or carbohydrates (animal or aquaculture feeds)
 agar (thickener)
carrageenan (stabilizer/emulsifier)
nutraceuticals (carotenoids, omega 3-6-7-9, anti-microbials, anti-fungals)</li></li></ul><li>Omega Oils – Omega 7<br />A P...
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2011 04 oa algae applications (web) connelly 2011

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2011 04 oa algae applications (web) connelly 2011

  1. 1. Algae Analytics and Nutraceutical Applications for Algae<br />Rhykka Connelly<br />r.connelly@cem.utexas.edu<br />April 27, 2011<br />
  2. 2. Standard Measurement Technologies<br />Nile Red is the current measurement tool of choice in the algae industry<br />Here’s what Nile red measures: lipophilic molecules <br />Here’s an example of how Nile red measurements can mislead algae growers:<br />Nile red measurements may indicate that both of these samples has 30% “oil” <br />
  3. 3. UT/OpenAlgae Technologies<br />Evaluating the intermediate products requires many analytical tools<br />
  4. 4. UT/OpenAlgae Technologies – Thin Layer Chromatography<br />Samples are taken before and after each processing step. Each sample is separated into a biomass pellet (P) and supernatant (S) fraction and analyzed by TLC against known standards.<br />
  5. 5. UT/OpenAlgae Technologies – Thin Layer Chromatography<br />Lipids released into the supernatant<br />Lipids remaining with the biomass pellet<br /><ul><li> Triglycerides remain relatively stable throughout processing, whereas diglycerides and free fatty acids rise during processing
  6. 6. Suggests that we’re recovering beneficial lipids from sources other than triglycerides…possibly membrane lipids</li></li></ul><li>UT/OpenAlgae Technologies – HPLC/MS<br />Quantitative Chemical Analysis of Oil Extraction Process<br />HPLC methods have been developed for the quantification of algal lipid classes using Evaporative Light Scattering Detection (ELSD) and Mass Spectrometry (MS)<br />Chl<br />More than 100 discrete ion species have been observed in lipid extracts using MS<br />Hydrocarbons (HC)<br />Prenol lipids (e.g.; β-carotene, BC)<br />Triacylglycerides (TAG)<br />Diacylglycerides (DAG)<br />Monoacylglyceride (MAG)<br />Free Fatty Acids (FFA)<br />Polar Lipids (i.e.; phospholipids)<br />polar lipids<br />neutral lipids<br />HC<br />Glycolipids<br />TAG<br />DAG<br />BC<br />MAGFFA<br />Polar Lipids<br />CEM Chlorella sp.<br />
  7. 7. UT/OpenAlgae Technologies – GC/MS<br />We can also track specific fatty acids, such as Omega 3-6-7-9, throughout growth and processing<br />GC/MS<br />(gas chromatography)<br /><ul><li> GC – identify lipid species by chain length </li></ul>Peak identification in GC profile above: (1) caprylic acid (C8:0); (2) capric acid (C10:0); (3) lauric acid (C12:0); (4) myristoleic acid (C14:1); (5) myristic acid (C14:0); (6) pentadecanoic acid (C15:0); (7) palmitoleicacid (C16:1); (8) palmitic acid (C16:0); (9) heptadecanoic acid (C17:0); (10) linoleic acid (C18:2n-6c); (11) oleic acid (C18:1n-9c); (12) -linolenic acid (C18:3n-3); (13) stearic acid (C18:0); (14) arachidonic acid (C20:4n-6); (15) eicosapentaenoic acid (C20:5n-3); (16) eicosenoic acid (C20:1); (17) arachidic acid (C20:0); (18) docosahexaenoic acid (C22:6n-3); (19) erucicacid (C22:1); (20) behenic acid (C22:0); (21) nervonic acid (C24:1); (22) hexacosanoic acid (C26:0); (23) octacosanoic acid (C28:0); (I.S.) tricosanoic acid (C23:0).<br />
  8. 8. UT/OpenAlgae Technologies – Analysis Conclusions<br /><ul><li> The algae industry has relied on crude quantitative technologies that can mislead growers, researchers, and investors
  9. 9. UT sets itself apart by incorporating cutting edge technologies that measure lipids individually
  10. 10. Adoption of these technologies will help increase understanding of algal lipid dynamics and standardize the way lipids are measured in the industry.</li></li></ul><li>The Diversity of Algae<br /><ul><li> There are ~100,000 species of algae
  11. 11. Certain kinds of algae make products that we’re interested in
  12. 12. oil for fuel
  13. 13. high in proteins and/or carbohydrates (animal or aquaculture feeds)
  14. 14. agar (thickener)
  15. 15. carrageenan (stabilizer/emulsifier)
  16. 16. nutraceuticals (carotenoids, omega 3-6-7-9, anti-microbials, anti-fungals)</li></li></ul><li>Omega Oils – Omega 7<br />A Possible Solution: Omega-7<br />The essential fatty acid palmitoleic acid (Omega-7) promotes the formation of new blood vessels and collagen deposition at the site of injury.<br /><ul><li> Currently, Omega-7 is primarily derived from Sea Buckthorn, a cold weather plant that is harvested once a year.
  17. 17. We have identified an algae that produces large quantities of Omega-7 and can be harvested daily. </li></ul>The problem: <br />Limited vascularization at the wound site<br />
  18. 18. Omega Oils – Commercially Available Omega 7 Wound Healing Study Preliminary Results<br />Wound Closure<br />Omega-7 accelerates wound closure<br />
  19. 19. Omega Oils – Commercially Available Omega 7 Wound Healing Study Preliminary Results<br />Blood Flow Due to Neovascularization<br />Scar Formation<br />Omega-7 extracts improve blood flow and minimizes scar formation in wounded sheep. <br />
  20. 20. Omega 7 – A Collaborative Approach<br />Algae Scale-up and Processing<br />In Vitro and in Vivo Study<br />The results of the study will be published, and if successful, be extended to industry.<br />
  21. 21. Algae Biomass Fertilizer – A Pilot Project<br />Measure:<br /><ul><li> Plant height
  22. 22. Circumference
  23. 23. Number of fruits/leaves produced
  24. 24. Soil analyses</li></ul>Algae<br />Commercial fertilizer<br />Control<br />
  25. 25. Other Algae Applications – Conclusions<br /><ul><li> In addition to biofuel oils, algae produce many “high-value” products
  26. 26. Some of the “high-value” products are billion dollar industries
  27. 27. UT has developed technologies that can cost-effectively recover biofuel oils and high-value products
  28. 28. Using CEM/OpenAlgae-developed technologies, we can collaborate with other research universities and private institutions to advance health applications
  29. 29. The processed algae biomass is useful too -- we have initiated an algae fertilizer pilot program on the UT campus
  30. 30. We continue to develop technologies useful to UT and industry</li></li></ul><li>Contact Information<br />Dr. Rhykka Connelly<br />Center for Electromechanics<br />Research Scientist<br />(512) 232-1604<br />r.connelly@cem.utexas.edu<br />Mr. Hoyt Thomas<br />OpenAlgae<br />President and CEO<br />(713) 979-2600<br />hhthomas@openalgae.com<br />Dr. Robert Hebner<br />Center for Electromechanics<br />Director<br />(512) 232-1628<br />r.hebner@cem.utexas.edu<br />
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