2011 04 oa algae applications (web) connelly 2011Presentation Transcript
Algae Analytics and Nutraceutical Applications for Algae Rhykka Connelly firstname.lastname@example.org April 27, 2011
Standard Measurement Technologies Nile Red is the current measurement tool of choice in the algae industry Here’s what Nile red measures: lipophilic molecules Here’s an example of how Nile red measurements can mislead algae growers: Nile red measurements may indicate that both of these samples has 30% “oil”
UT/OpenAlgae Technologies Evaluating the intermediate products requires many analytical tools
UT/OpenAlgae Technologies – Thin Layer Chromatography Samples are taken before and after each processing step. Each sample is separated into a biomass pellet (P) and supernatant (S) fraction and analyzed by TLC against known standards.
UT/OpenAlgae Technologies – Thin Layer Chromatography Lipids released into the supernatant Lipids remaining with the biomass pellet
Triglycerides remain relatively stable throughout processing, whereas diglycerides and free fatty acids rise during processing
Suggests that we’re recovering beneficial lipids from sources other than triglycerides…possibly membrane lipids
UT/OpenAlgae Technologies – HPLC/MS Quantitative Chemical Analysis of Oil Extraction Process HPLC methods have been developed for the quantification of algal lipid classes using Evaporative Light Scattering Detection (ELSD) and Mass Spectrometry (MS) Chl More than 100 discrete ion species have been observed in lipid extracts using MS Hydrocarbons (HC) Prenol lipids (e.g.; β-carotene, BC) Triacylglycerides (TAG) Diacylglycerides (DAG) Monoacylglyceride (MAG) Free Fatty Acids (FFA) Polar Lipids (i.e.; phospholipids) polar lipids neutral lipids HC Glycolipids TAG DAG BC MAGFFA Polar Lipids CEM Chlorella sp.
UT/OpenAlgae Technologies – GC/MS We can also track specific fatty acids, such as Omega 3-6-7-9, throughout growth and processing GC/MS (gas chromatography)
Omega Oils – Omega 7 A Possible Solution: Omega-7 The essential fatty acid palmitoleic acid (Omega-7) promotes the formation of new blood vessels and collagen deposition at the site of injury.
Currently, Omega-7 is primarily derived from Sea Buckthorn, a cold weather plant that is harvested once a year.
We have identified an algae that produces large quantities of Omega-7 and can be harvested daily.
The problem: Limited vascularization at the wound site
Omega Oils – Commercially Available Omega 7 Wound Healing Study Preliminary Results Wound Closure Omega-7 accelerates wound closure
Omega Oils – Commercially Available Omega 7 Wound Healing Study Preliminary Results Blood Flow Due to Neovascularization Scar Formation Omega-7 extracts improve blood flow and minimizes scar formation in wounded sheep.
Omega 7 – A Collaborative Approach Algae Scale-up and Processing In Vitro and in Vivo Study The results of the study will be published, and if successful, be extended to industry.
Algae Biomass Fertilizer – A Pilot Project Measure:
Number of fruits/leaves produced
Algae Commercial fertilizer Control
Other Algae Applications – Conclusions
In addition to biofuel oils, algae produce many “high-value” products
Some of the “high-value” products are billion dollar industries
UT has developed technologies that can cost-effectively recover biofuel oils and high-value products
Using CEM/OpenAlgae-developed technologies, we can collaborate with other research universities and private institutions to advance health applications
The processed algae biomass is useful too -- we have initiated an algae fertilizer pilot program on the UT campus
We continue to develop technologies useful to UT and industry
Contact Information Dr. Rhykka Connelly Center for Electromechanics Research Scientist (512) 232-1604 email@example.com Mr. Hoyt Thomas OpenAlgae President and CEO (713) 979-2600 firstname.lastname@example.org Dr. Robert Hebner Center for Electromechanics Director (512) 232-1628 email@example.com