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Frolicking in France by Brittany Hubert

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  • 1. Frolicking in FranceSummer Enrichment Trip 2011
    By Brittany Hubert
  • 2. Getting Started
    Interested in Molecular Biology
    Medical School?
    Not Really..
    Research?
    I think so…
    Started Lab Work with Dr. Perlin
    Growing Interest in Research
  • 3. My Project
    Biology Research from different aspects
    University lab, service work, research facility, other..??
    Different Areas of Molecular Biology
    Virology, Immunology, Bacteria, Fungus
    Find what fits me
    Gain experience in all areas
    Learn to think like a “scientist”
    Use experience to advance in my personal education experience
    Form lasting relationships with experienced professionals
  • 4. Summer 2011
    Seven weeks at University of South Paris
    Orsay, France
    About 13 miles outside of Paris
    Work with Tatiana Giraud PhD and lab team
    The fungus, Microbotryum
    3 Goals of Project
    Learn as much as possible about plant infections, microsatellites, and inoculations
    Gain general lab experience
    Immerse myself in French culture and practice language for possible minor
  • 5. Gettin’ Down to Business
    Fungus Microbotryum:
    Anther Smut Disease
    Infects anthers of flowers in carnation family (Silenelatifolia & Silenedioica)
    Topics
    Glacial refugia using microsatellites- repeated units of DNA characteristic of different strains
    Hybrid Sterility/Gene Flow between and within clusters
    Competetive Exclusion
    My Jobs
    PCR with Odile and Pierre (microsatellites)
    Making and recording conjugations
    Garden work with Laetitia
    Replating and recording good strains
    Healthy
    Infected
    “Smutted”
  • 6. My Lab Team!
    Odile
    Pierre
    Mandy
    Tatiana
    Alex
    Marina
    Elsa
  • 7. Microbotrym in a Nutshell..or Meristem
    The Lab
    • Lab techniques
    • 8. PCR, Pipetting, Conjugations
    • 9. Working with a Team
    • 10. Reading (and understanding) research articles
    • 11. Making Charts
    • 12. Reasoning
    Microbotryum
    Easily cultured in lab
    Reproduces quickly
    Grows easily on petri dishes
    Spread by pollinators in nature
    (bees, bugs..etc)
    Infects only meristems-embryonic tissue in plants; undifferentiated, growing, actively dividing cells.
    (dictionary.com)
    Host specific
    Followed same speciation path as hosts after glaciations
    High selfing rate/low crossing rates
    Closely related species can reproduce
    Genetic distance determines reproductive ability
  • 13. Why it Matters!
    Allows us to:
    Understand other past disease patterns
    Predict future patterns
    Understand interactions between host-specific or parasitic organisms
    Start work on disease prevention or cures
    Glacial Refugia
    Silenelatifolia
    Smut Life Cycle
    Silenedioica
  • 14. Biology on the Beach
    Four Day Biology Conference
    In Roscoff (Brittany), France
    On New and Emerging Fungal Diseases of Animals and Plants in the context of evolution
    Interesting talks on many different areas
    Eye-opening
    Confusing
    Learned
    What conferences are all about
    New material
    What I don’t know..
    Met many important people/Made new acquaintances
    Spent a day on the beach!
  • 15. Things you learn when you travel completely alone!
    Goals
    • Gain more self-confidence-check!
    • 16. Become more independent-check!
    • 17. Expand my comfort zone-check!
    How (and who) to ask for help
    How to use multiple maps
    What it takes to entertain myself
    How interesting other people’s lives can be
    The Paris metro system (after a while)
    Exactly what you like and don’t like
    How to adjust to different cultures
    Travel to France, Switzerland, Germany, and Rome
  • 18. Goals for the Future
    Continue work in Dr. Perlin’s lab
    Explore different areas of Molecular Biology research
    Not lab work (monotonous)
    More dynamic area
    Possible work with Pasteur Institute or Marie Curie institute in Africa
    Look into forensic fields
  • 19. Special thanks to the James Graham Brown Foundation!!
    And thanks to:
    -Andrew Grubb
    -Dr. Michael Perlin
    Pictures with no citation are from google.com or my camera!