Microscopy101

Microscopy 101

Proper Handling
Make sure cord is secure
Support the base with one hand
Lift with the arm of the scope
Do not swing!

Ocular Arm Stage Base Coarse Adjustment Fine Adjustment Light Source Diaphragm Stage Clips Objectives Nosepiece Tube

Magnification Ocular = 10x 4x Objective 10x Objective 40x Objective Therefore, 40x, 100x, 400x Magnification

Getting Started
Plug in & turn on
Rotate nosepiece to place 4x objective into position
Raise stage all the way up by turning the coarse adjustment knob
Place specimen slide into place
Look into ocular as you lower the stage VERY SLOWLY until low magnification image focuses

Increasing Magnification & Focusing
Once low magnification is focused, DO NOT touch coarse adjustment knob ever again!
Switch to 10x objective by rotating nosepiece
Use fine adjustment knob to focus
Once focused, switch to 40x objective to view high magnification
Focus with fine adjustment knob

Before you leave…
Return slides to proper boxes
Turn off & unplug scopes
Rotate to lowest objectives (4x)
Raise stage all the way up with coarse adjustment knob
Secure cords
Return to cabinets, carrying properly

Microscopy101

by Brad Hanson on Jan 20, 2010

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Microscopy101 — Presentation Transcript