PREPARED BY
B.SUNIL REDDY
I M.PHARM
PHARMACEUTICS
Definition:
Chromatography is a technique for separating
mixtures into their components in order to
analyze, identify, pur...
Invention of chromatography
Micheal Tswett invented
the chromatography in
1901 during his research
on plant pigments.
He u...
Components of Chromatography
Mobile Phase – gas or liquid that carries the
mixture of components through the stationary
ph...
Uses for Chromatography
Chromatography is used by scientists to:
•Analyze – examine a mixture, its components,
and their r...
Uses for Chromatography
Real-life examples of uses for chromatography:
Pharmaceutical Company
Hospital
Law Enforcement
...
Types of Chromatography
•Liquid Chromatography – separates liquid samples with a
liquid solvent (mobile phase) and a colum...
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
Chromatography
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Chromatography

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Chromatography

  1. 1. PREPARED BY B.SUNIL REDDY I M.PHARM PHARMACEUTICS
  2. 2. Definition: Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components. Mixture Separat e  Analyze • Identify • Purify • QuantifyComponent s CHROMATOGRAPHY
  3. 3. Invention of chromatography Micheal Tswett invented the chromatography in 1901 during his research on plant pigments. He used the technique to separate various plant pigments such as chlorophyll, xanthophylls, Carotenoids. Micheal Tswett Russian botanist 1872-1972
  4. 4. Components of Chromatography Mobile Phase – gas or liquid that carries the mixture of components through the stationary phase. Stationary Phase – the part of the apparatus that holds the components as they move through it, separating them.
  5. 5. Uses for Chromatography Chromatography is used by scientists to: •Analyze – examine a mixture, its components, and their relations to one another •Identify – determine the identity of a mixture or components based on known components •Purify – separate components in order to isolate one of interest for further study •Quantify – determine the amount of the a mixture and/or the components present in the sample
  6. 6. Uses for Chromatography Real-life examples of uses for chromatography: Pharmaceutical Company Hospital Law Enforcement Environmental Agency Manufacturing Plant
  7. 7. Types of Chromatography •Liquid Chromatography – separates liquid samples with a liquid solvent (mobile phase) and a column composed of solid beads (stationary phase) • Gas Chromatography – separates vaporized samples with a carrier gas (mobile phase) and a column composed of a liquid or of solid beads (stationary phase) •Paper Chromatography – separates dried liquid samples with a liquid solvent (mobile phase) and a paper strip (stationary phase) •Thin-Layer Chromatography – separates dried liquid samples with a liquid solvent (mobile phase) and a glass plate covered with a thin layer of alumina or silica gel (stationary

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