Common-use Data System for Food
Animal Genetics in Taiwan
Ming-Che Wu
Taiwan Livestock Research Institute

A reservoir of genetic adaptability acts a buffer
against potentially harmful environmental and
economic change.
Food security and mileage (Sustainability)

System for Food Animal Genetics
in Taiwan
Phase I (1987~1992)：
Conservation farms
●
Conservation farms
Phase II(1993~1998)：
● Web site www.angrin.tlri.gov.tw
Web site
Phase III(1999~2004)：
Cryopreservation Bank
●
Conservation center
Phase IV(2005~2008)：
Molecular techniques
●
Research and Utilization
Genetic resources Phase V(2009~2012):
●
exchange Global Bioidentification System

Common-use
• DNA in food chain
• Biogenetic banks in
supporting scientific
research
• Genomic selection on
farm level
• Web-based barcode
system with food mileage

乳 Milk
DNA in food chain
Meat 肉
Egg 蛋

Origin
assurance
- market to farm

Web site: www.angrin.tlri.gov.tw

BioUtilization
Conservation Biodiversity
Species
Species
verification
Value add
Selection Demand
New
breed/line
Tracking
Export
Genetic
improvement
Genomic breeding

(source:FAO, 2007)
Global Movement of AnGR
(milk)
(pork)
(pork)
(pork)
(milk)
(milk)
(chicken meat)
(chicken egg)
(fur sheep)

Biogenetic resources are parts of our life.
Genetic diversity is threatened by
- genetic erosion
- habitat loss
- social value sinking
- economic setback
- food print mileage
From Farm to DNA Bank

Genetic
Banking
Living form
Cell/tissue
DNA/RNA/mRNA/
cDNA/protein

Development of miniature pig for biomedical research in Taiwan
Ming-Che Wu, PhD
Taiwan Livestock Research Institute
www.angrin.tlri.gov.tw
mcwu@mail.tlri.gov.tw
Taiwan
Lanyu

Development of miniature pig for biomedical research in Taiwan
Ming-Che Wu, PhD
Taiwan Livestock Research Institute
www.angrin.tlri.gov.tw
mcwu@mail.tlri.gov.tw
Lanyu 200
Hybrid
Hybrid
Inbreeding Duroc
GPI-CRC-PGD Genotyped Mitsae (Lanyu 50)
Spotty Lanyu
Line (Lanyu 300) (Lanyu 100) (50% Lanyu + 50% Duroc)

Mitasi minipig was developed from sib-mating on hybrid of Duroc
and Lanyu breed.
1989
1990
1991
1992
1993
1994
1995
(Lanyu 50)
50

Fullsib Mating on
coat color selection
19

Development of miniature pig for biomedical research in Taiwan
Ming-Che Wu, PhD
Taiwan Livestock Research Institute
Lanyu 300 www.angrin.tlri.gov.tw
mcwu@mail.tlri.gov.tw
Lanyu 50
Lanyu 100
Lanyu 200
Conservation to Bio-
utilization (1987-2008) Lanyu 300
Lanyu 100
Lanyu
Lanyu 100 Lanyu 400
Lanyu 50
Black
1980
Lanyu White
Spotty Lanyu
2005
1993

Semen collection and storage
Ex situ conservation of minipig

• Cell Bank
- Sperm bank, Oocyte bank, Embryo bank
• DNA Bank
Taiwan Animal Germplasm Center
http://www.angrin.tlri.gov.tw/

Taiwan Animal Germplasm Center
http://www.angrin.tlri.gov.tw/

CryoArk for Taiwan
Cryotanks: 24K*5 + 38K*17 + 80K*2 = 926K
(CryoArk for Taiwan: 46,000 sp. in total)

Oocyte
Sperm
Embryo
Cell Bank

•K Series - Liquid nitrogen storage with controllable
temperatures between (-100°C to -196°C)
Small LN Tank
-185℃ Vapor
storage
•designed for large
vial capacities up
to 6000 vials (2 ml
vial) in box type
racks.
-80℃ Freezer

Bio-security
-185℃ Vapor
storage
•designed for large
vial capacities up to
6000 vials (2 ml vial) in
box type racks.

Gene Banking
DNA Banking
2007 Living form/Cell/Tissue
DNA/RNA/mRNA/cDNA/Protein
Cattle 6180
Water Buffalo 58
Goat 3127
Oocyte
Sheep 14
Horse 14
Rabbit 88
Swine 25129
Chicken 11650
Muscovy 151
Embryo
Duck 689
Hybrid Duck 128
Goose 431
Turkey 14
Black Swan 135
Quail 50
Total 47858

Landrace Lanyu Duroc Taoyuan
Lee-Sung Inbreeding Mitsae TLRI Black
Spotty
Meishing
Yorkshire
Hampshire Berkshire
LY
Pig Breeding
LYD Hog

Impact of common-use breeding data on production efficiency
2.20
Duroc Landrace Yorkshire
2.15 Feed efficiency(Feed/Gain) from 40 to 110 kg of body weight
2.10
2.05
2.00
2000 2001 2002 2003 2004 2005 2006 2007
Birth year of boar

Growth performance test for breeding pig

Auction

Elite animal labeled with the breeder name
prior to auction

Performance data of individual pig with its own pedigree along with genotype
information
Auction price of each pig and buyer soon after sold

On-line Statistics for Auction Data of Elite Pig
Auction Price (NT$)
No. of Breeder and Buyer
Year

Cow Milk Production in Dairy Herd Improvement System

Regional Statistics on Dairy Farming Sector
Cow Milking Performance
No. of Farmers
County

Profiles of daily milk yield per cow in average and number of DHI farms
(1990 ~ 2008)
400
25
Daily Milk Yield (kg)
24
DHI Farm (N)
350
23
22
300
21
250
20
19 4.6
4
3.8
4.4
200
3.6
4.2
3.4
18 3.2
4
3
3.8
2.8
Profile on milk quality in
Protein % Protein % (milk yield adjusted)
2.6
3.6
2.4
17 protein percentage from
2.2 3.4
150
those farms having more
2
3.2
1.8
than 22 kg of milk yield
1.6 3
16 1.4
recorded in 2007.
2.8
1.2
1 2.6
.1
.2
.07
.14
.32
.37
.39
.59
.77
.86
.15
.39
.45
.52
.55
.76
.81
.04
.11
.24
.36
.62
.69
.79
.92
.07
.17
.24
.52
.97
.28
.51
.57
.28
.42
.71
.74
.05
.16
.51
23
29
22
22
22
22
22
22
22
22
23
23
23
23
23
23
23
24
24
24
24
24
24
24
24
25
25
25
25
25
26
26
26
27
27
27
27
28
30
34
15 100
07
11
03
07
11
03
07
11
03
07
11
03
07
11
03
07
11
03
07
11
03
07
11
03
07
11
03
99
99
00
00
00
01
01
01
02
02
02
03
03
03
04
04
04
05
05
05
06
06
06
07
07
07
08
19
19
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20

Regulation for genomic breeding - Animal Industry Act Article 17
The competent authority may dispatch inspector to examine or test the breeding
flock, breeding stock, facilities, pedigree registry and related records of breeders, and
the breeders shall not evade, interfere with or object to such examination or testing.
Breeding flock or breeding stock found to have contracted notified disease or have
hereditary disease during the aforesaid examination or testing shall be banned from
breeding. The aforesaid inspectors shall present their identification when carrying out
their duty.
Pig Cow Goat Chicken Duck Goose Deer Rabbit
Hal-1843 BLAD G6S HSP70 PRL PRL IGF2 GPI
ESR DUMP IGF2 PRLR ESR ESR XY PGD
Gene
HFABP-H CITR mtDN PRL ZW ZW D5FAD XY
A
HFABP-ad CVM ESR mtDNA mtDNA mtDNA IGF2
IGF2-In3 XY mtDNA mtDNA
IGF2-In7 GH ZW
D5FAD mtDNA
D-Loop
SW18
SW1943
SW2588
PRLR

DNA barcoding for pigs
DNA
Hal-1843 gene for stress (AB), ESR gene for prolificacy
(MN) and 16,613bp mtDNA for maternal origin,
especially on D-loop region (1,175bp) of 29 SNPs and
UTRn (tacacgtgcg 10bp)17~27 in various breeds of pigs.
AB+MN+18SNP+UTR+11SNP
mtDNA D-Loop
AAMNcaattgctcgtttctcag24tgctcgaaacc
A0021-02 Lanyu
T0596-01 Taoyuan AAMMcaattgcttgttttccag24tgctcgaaact
L0074-10 Landrace AAMNttgccattcaccctccaa27tgcttaaaatt
Y0150-03 Yorkshire AAMNcaattgcctgttttccag25tccccgaaacc
AANNttgccattcaccctccaa26tgcttaaaatc
D0167-05 Duroc

Native Chicken Barcode
MMFFAARR
2-3-5-Z (Red feather)
PRL-ESR-HSP70-PRLR
NNEEBBPP
Chicken genome
(Black feather)
(Silky feather/Black meat)
2-3-5-Z-mtDNA
PRL-ESR-HSP70-PRLR-CoI
MMEEAAPP CCGGTCC Broiler

Common-use
• DNA in food chain
• Biogenetic banks in
supporting scientific
research
• Genomic selection on
farm level
• Web-based barcode
system with food mileage

Acknowledgements • Wu Lab
– Mr. Yun-Yu Lai
– Dr. Ren-Bao Liaw
• –
Funding Sponsors Dr. Yuchia Huang
–
– COA Dr. Ter-Yu Lin
–
– TLRI Ms. Hsiu-Rong Tsai
– Mr. Hsenn-Ben Chu
– NAIF
• Collaborators
– Prof. Hsiu-Luan Chang
– Dr. Lih-Ren Chen
– Prof. Feng-Pang Cheng
– Prof. Larry Schook, UIUC
– Prof. Harris Lewin, UIUC
– Dr. Le Thi Thuy, Vietnam
– Prof. Rangsun Parnpai, Thailand
– Dr. Libertado C. Cruz, Philippines
– Prof. Chis Maxwell, Australia
– Prof. Locksley E. McGann, Canada
– Prof. Hoon Taek Lee, Korea