Mechanism of enzyme action

EDT 5303 TECHNOLOGY ANDINNOVATION IN EDUCATION ( GROUP 12) TOPIC : MECHANISM OF ENZYME ACTION 1 . N U R F AU ZAN A BT ZU LKIF LI (G 1 027668) 2. N O R ‘AID AH BT LU K M AN (G 1 02731 2) 3. WAN S U H AILA BT WAN IS M AIL (G 1 022282 )

C O N TE N T LEARNING1 OUTCOME2 INTRODUCTION PRIOR3 KNOWLEDGE DEFINITION OF4 ENZYME MECHANISM OF5 ENZYME ACTION6 ASSESSMENT

LE AR N IN G O U TC O M E 4.10 : Explain the mechanism of enzyme action

IN TR O D U C TIO N Do you realize the term of COENZYME on detergent products? CLICK HERE !!

P R IO R K N O WLE D G E RECALL !!!Maltose + Water Glucose + Glucose ENZYME maltase BREAK DOWN maltose into 2 molecules of glucose

P rior knowle d ge Enzyme s Cloth StainsHow the stains have beenremoved from the cloth?

D E F IN ITIO N O F E N ZYM E Enzymes are biologicalcatalyst that direct or guidealmost all cellular reactions.

M E C H AN IS M O F E N ZM E AC TIO N ‘LOCK AND KEY’ HYPOTHESIS

M E C H AN IS M O F E N ZM E AC TIO N Product ProductEnzyme Enzyme-substrate complexEXPLANATION EXPLANATION EXPLANATION Substrate

M E C H AN IS M O F E N ZM E AC TIO NA specific substrate moleculearrives at the active site of the enzyme molecule.

M E C H AN IS M O F E N ZM E AC TIO NThe substrate molecule binds to the active site to form an enzyme-substrate complex which is very unstable.

M E C H AN IS M O F E N ZM E AC TIO NProduct molecules leave the active site of the enzyme. The enzyme is free to take another substrate moleculeinto its active site to catalyse another reaction.

M E C H AN IS M O F E N ZM E AC TIO NWhich substrate can bind to active site of enzyme sucrase? Sucrose can bind to active site of sucrase Enzymes have specific sites called Sucrose active site which bind to specific substratesSucrase Maltose Maltose CANNOT bind to active site of sucrase

as s e s s m e ntQuestion 1 Question 2Question 3 Question 4

Q u e s tion 1 Fill the blank with the correct answer.2. A specific ______________ molecule arrives Product at the active site of the ______________ molecule. enzyme- substrate2. The substrate molecule binds to the active site of the complex enzyme to form an ______________. enzyme3. The enzyme is free to take another substrate molecule active site into its ______________ to catalyse another reaction. substrate4. ______________ molecules leave the active site of the enzyme.

Q u e s tion 2Which of the following shows the characteristic of enzymes? A. Enzyme reaction is specific A. Enzyme reaction is influenced by pH C. Enzyme reaction is influence by temperature D. The structure of the enzyme change at the end of the reaction.

Q u e s tion 3Protease Dissolve starch stains on clothAmylase Ripening cheeseLipase Dissolve protein stains on cloth

Q u e s tion 4 Enzyme is used to wash away the blood stain on a shirt. Explain how the enzyme acts on the blood stain at a maximum rate. (3 MARKS) ANSWER : Blood is made up from proteins. Protease in detergentwill break down protein into amino acids. This reactionoccur effectively at 40 ºC which is optimum temperature for enzyme action.

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Mechanism of enzyme action

by bioteachers on Jun 07, 2012

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