3D liver                      microtissuesToxicology Products                        » Liver-specific morphology and high ...
What are 3D liver microtissues?Liver microtissues are multicellular spheroids of hepatocyte cells either in monoculture, o...
Natural               environment         Advantages of InSphero 3D Liver Microtissues                      Native-like ti...
Services Overview                                                                                                         ...
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3D Liver Microtissues ready-to-assay

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Products toxicology web

  1. 1. 3D liver microtissuesToxicology Products » Liver-specific morphology and high functionality over 21 days » Chronic in vitro toxicity studies possible » Assay-ready, no cell culturing needed » No scaffolds, so no unwanted artifacts » Supplied in automation-friendly 96 well-format
  2. 2. What are 3D liver microtissues?Liver microtissues are multicellular spheroids of hepatocyte cells either in monoculture, or specially de-signed in co-culture systems. These scaffold-free tissue models display remarkably similar histomorpho-logical, functional and microenvironmental features to in vivo liver tissue.3D liver microtissues are ideal for toxicology and metabolic analysis and are far more likely to be predic-tive of adverse toxicological effects compared to sandwich cultures. At last these valuable 3D modelsare conveniently available on demand in large quantities, ready-for-use in high-throughput applications. How are InSphero 3D tumor microtissues made? InSphero’s GravityPLUS process gently coerces cells to assemble into microtissues in a very reproducible 2D/3D cell culture comparison mass-production process. This process generates assay-ready 96 well plates, each containing one micro- 2D Sandwich 3D Microtissue tissue per well. By combining hepatocytes with other cells, such as liver-derived non-parenchymal cells,Scaffold Yes No highly organotypic liver microtissues can be produced that retain liver-specific morphology and functio-Co-culture – +++ nality for more than 21 days.Long term functionality + +++Bile canalicular network ++ +++Cell-cell contacts + +++Differentiation + +++High-throughput analysis + +++High-content analysis ++ ++
  3. 3. Natural environment Advantages of InSphero 3D Liver Microtissues Native-like tissue: Hepatocytes in 3D microtissues maintain a polarized structure, form bile canaliculi and maintain native-like functionality for extended periods. Faster and easier: Available in industrial-scale quantities, in standard 96-well format, ready for screen- ing or other high-throughput applications, eliminating the effort required to produce 2D sandwich cultures. Compatible with established assays: No concerns about compatibility with current approved assays. No scaffolds: Artificial scaffolds are not used which means freedom from concerns about unknown and unwanted assay artefacts due to scaffold interactions with drug compounds, assay reagents or cells. Longer culture lifetime: Whereas 2D sandwich cultures offer only a very narrow time window for gathering data this is extended many-fold with InSphero’s liver microtissues (21 days or longer).© HLPhoto - Fotolia www.insphero.com
  4. 4. Services Overview For feasibility studies or compound testing offers using our rat-liver microtissues please contact our sales department at sales@insphero.com Product OverviewAssay-ready liver microtissuesAssay-ready microtissues delivered in a specially coated, non-adhesive, 96-well V-bottom plate:Cat. # Name Description Cell sourceMT-02-001-04 rLiMT Rat liver microtissues formed by primary Wistar hepatocytes in co-culture with non-parenchymal cells InSphero AGMT-02-002-01 hLiMT Human liver microtissues formed by fresh Lonza Technoparkstrasse 1 human hepatocytes (homotypic culture) CH-8005 ZurichMT-02-002-03 hLiMT Human liver microtissues formed by cryo- Lonza Switzerland preserved human hepatocytes in co-culture with normal human dermal fibroblasts www.insphero.com Phone: +41-44-515049-0 e-mail: sales@insphero.comAll technical and pricing information subject to change.All products are for research use only. They are not intended for any animalor human therapeutic or diagnostic use unless otherwise stated.

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