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Presentation Sirion Web V7 Spoken

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SIRION BIOTECH Portfolio

SIRION BIOTECH Portfolio

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  • Virus vectors for basic research and vaccine development include Adenovirus vectors, Adeno-associated virus vectors, and lentivirus vectors for experimental gene transfer and target validation in cell culture and in vivo. Viral vectors produced by Sirion are safe and replication defective and can be used in vitro or supplied as high titer stocks for use in vivo. Sirion specializes in customized viral vector construction and production, but also supplies vectors direct from stock.. Each vector type has its individual benefits and application spectrum:

Presentation Sirion Web V7 Spoken Presentation Sirion Web V7 Spoken Presentation Transcript

  • Experts in Gene Silencing and Over-expression
    Delivering ANY Gene to ANY Cell
  • Sirion Offers
    Shorter Timelines and
    Improved Quality
    Custom development of genetically modified mammalian cells
    Cell line optimization services
    Cell line immortalization services
  • Sirion Delivers
    Shorter Timelines and
    Improved Quality
    Functionally tested, ready-to-use tailor-made genetically modified cells,
    Guaranteed gene delivery into virtually any mammalian cell type
    Guaranteed > 90% knockdown maintained over the required experimental time frame
    Results in weeks instead of the months needed for in-house solutions
  • Our expertise
    Viral vectors ─ Adenovirus, AAV, Lentivirus
    • For cell research and vaccine development
    Genetically modified cell systems for target validation
    • Cellular models for oncology
    • Ready-to use frozen cells
    • Immortalization of primary human cells
    Optimization of production cells
    • Rational target identification by dynamic mRNA profiling
  • Our expertise: Virus technology
    AAV
    Adenovirus
    Lentivirus
    Basic Research
    Vaccine Development
    Safe, replication defective vectors
    Custom construction and production, or direct supply from stock
    High titer stocks for in vivo use available
  • Guaranteed custom virus vectors
    Custom virus vector construction
    • shRNA expression with certified knockdown efficiency
    • Flexible choice of promoters and expression cassettes
    • Expression up to 4 genes in one vector possible
    • QC, RCA-tested vector preparations
    High titer virus stocks for in vivo
    Your Benefits
    All vectors are guaranteed for function
    S1/BSL1 ready-to-use, pre-transduced, frozen cell stocks available
    Fast time lines ─ 4 weeks from gene to vector
    SIRION-Viral vectors
    SIRION BIOTECH
    State of the art transfection
    Molecular Myology, LMU Munich
  • 20 to 50 fold higher gene transfer
    TransMAX Transduction Reagent
    GFP Transduction of NIH-3T3
    without with TransMAX
    • 20 to 50-fold enhanced adenovirus-mediated gene expression
    • Increased gene transfer in vivo
    • Accessibility of cell types
    MOI 200 MOI 200
    NIH-3T3 cells were transduced with an Adenovirus Serotype 5 expressing EGFP with a MOI of 200 without and in the presence of TransMax.
  • Our expertise: Cell technology
    • High performance shRNAs with >90% knockdown efficiency
    • Inducible knockdown via partnership with TET systems
    shRNA design and
    cloning into Sirion screening system
    Determination of
    knockdown efficiency by QPCR
    Confirmation of knockdown
    on the protein level
    by Western blot
    Ad368-high
    performance
    shRNA
    >90% KD
  • >90% knockdown – Guaranteed!
    Shorter Timelines and
    Improved Quality
    High performance viral vectors with guaranteed >90% gene knockdown
    Stable or inducible knockdown cells for reliable results
    Short timelines save money compared to long in house development
    Sirion’s experienced team can quickly generate the cell model you need
  • More predictive screening
    Primary cell line generation
    • Permanent Human Primary Cell immortalization
    • Polyclonal or monoclonal cell line generation
    • Phenotypic and functional characterization
    Conditional primary cell immortalization
    • The immortalizing gene is active at 33°C / inactive at 37°C
    • Conditionally immortalized cells terminally differentiate with close to primary cell type phenotype and function for more reliable results.
    * hTERT requires a license from Geron Inc.
  • Validated frozen cells direct to your lab
    • Validated gene knock-down or over expression
    • Guaranteed cell viability >90%
    • Standardized product (cell passage number, cell physiology, gene expression / knock-down)
    Applications
    • Infectious disease research
    • Drug candidate screening
    • Target validation in vivo (tumor models)
    • Production cell line optimization
  • Our expertise: Cell line optimization
    Optimization of production cell lines for vaccines and antibodies
    Target Gene
    Identification
    Mission®
    Cells
    Functional
    Testing
    Production
    Cell Line
    Target
    Validation
    Target
    Identification
    Cellular
    Assays
    Optimized
    Production cell
    • Rational choiceof target genes by dynamic mRNA profiling
    • Identification ofcritical cellular pathways for productivity
    • Over-expression or knockdown of target genes
    • Stable genetic modification
    • Antibody yield (ELISA testing)
    • Virus yield (PFU/IU testing)
    • Cellular assays /enzyme activity
    • Culture medium optimization
    • Genetic cell modification
    • Gene Silencing
  • Sirion Delivers
    Shorter Timelines and
    Improved Quality
    Functionally tested, ready-to-use tailor-made genetically modified cells,
    Guaranteed gene delivery into virtually any mammalian cell type
    Guaranteed >90% knockdown maintained over the required experimental time frame
    Results in weeks instead of the months needed for in-house solutions
  • Experts in Gene Silencing and Over-expression
    Delivering ANY Gene to ANY Cell
    SIRION BIOTECH GmbH Tel: +49 89 700 961 999Am Klopferspitz 19 Fax: +49 89 700 961 99882152 Martinsried info@sirion-biotech.comGermany www.sirion-biotech.com