Experts in Gene Silencing and Over-expression<br />Delivering ANY Gene to ANY Cell<br />
Sirion Offers<br />Shorter  Timelines and<br />Improved Quality<br />Custom development of genetically modified mammalian ...
Sirion Delivers<br />Shorter  Timelines and<br />Improved Quality<br />Functionally tested, ready-to-use tailor-made genet...
Our expertise<br />Viral vectors  ─ Adenovirus, AAV, Lentivirus<br /><ul><li>   For cell research  and vaccine development...
   Ready-to use frozen cells
   Immortalization of primary  human cells </li></ul>Optimization of production cells<br /><ul><li>   Rational target iden...
Guaranteed custom virus vectors<br />Custom virus vector construction<br /><ul><li>shRNA expression with certified knockdo...
Flexible choice of promoters and expression cassettes
 Expression up to 4 genes in one vector possible
QC, RCA-tested vector preparations</li></ul>High titer virus stocks for in vivo <br />Your Benefits<br />All vectors are g...
20 to 50 fold higher gene transfer<br />TransMAX Transduction Reagent<br />GFP Transduction of NIH-3T3<br />       without...
Increased gene transfer in vivo
Accessibility of cell types </li></ul>        MOI 200    	         MOI 200<br />NIH-3T3 cells were transduced with an Aden...
Our expertise: Cell technology<br /><ul><li>High performance shRNAs with >90% knockdown efficiency
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Presentation Sirion Web V7 Spoken

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SIRION BIOTECH Portfolio

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  • Virus vectors for basic research and vaccine development include Adenovirus vectors, Adeno-associated virus vectors, and lentivirus vectors for experimental gene transfer and target validation in cell culture and in vivo. Viral vectors produced by Sirion are safe and replication defective and can be used in vitro or supplied as high titer stocks for use in vivo. Sirion specializes in customized viral vector construction and production, but also supplies vectors direct from stock.. Each vector type has its individual benefits and application spectrum:
  • Presentation Sirion Web V7 Spoken

    1. 1. Experts in Gene Silencing and Over-expression<br />Delivering ANY Gene to ANY Cell<br />
    2. 2. Sirion Offers<br />Shorter Timelines and<br />Improved Quality<br />Custom development of genetically modified mammalian cells<br />Cell line optimization services<br />Cell line immortalization services<br />
    3. 3. Sirion Delivers<br />Shorter Timelines and<br />Improved Quality<br />Functionally tested, ready-to-use tailor-made genetically modified cells, <br />Guaranteed gene delivery into virtually any mammalian cell type<br />Guaranteed > 90% knockdown maintained over the required experimental time frame<br />Results in weeks instead of the months needed for in-house solutions<br />
    4. 4. Our expertise<br />Viral vectors ─ Adenovirus, AAV, Lentivirus<br /><ul><li> For cell research and vaccine development</li></ul>Genetically modified cell systems for target validation<br /><ul><li> Cellular models for oncology
    5. 5. Ready-to use frozen cells
    6. 6. Immortalization of primary human cells </li></ul>Optimization of production cells<br /><ul><li> Rational target identification by dynamic mRNA profiling</li></li></ul><li>Our expertise: Virus technology<br />AAV<br />Adenovirus<br />Lentivirus<br />Basic Research<br />Vaccine Development<br />Safe, replication defective vectors<br />Custom construction and production, or direct supply from stock<br />High titer stocks for in vivo use available<br />
    7. 7. Guaranteed custom virus vectors<br />Custom virus vector construction<br /><ul><li>shRNA expression with certified knockdown efficiency
    8. 8. Flexible choice of promoters and expression cassettes
    9. 9. Expression up to 4 genes in one vector possible
    10. 10. QC, RCA-tested vector preparations</li></ul>High titer virus stocks for in vivo <br />Your Benefits<br />All vectors are guaranteed for function<br />S1/BSL1 ready-to-use, pre-transduced, frozen cell stocks available<br />Fast time lines ─ 4 weeks from gene to vector<br />SIRION-Viral vectors<br />SIRION BIOTECH<br />State of the art transfection<br />Molecular Myology, LMU Munich<br />
    11. 11. 20 to 50 fold higher gene transfer<br />TransMAX Transduction Reagent<br />GFP Transduction of NIH-3T3<br /> without with TransMAX<br /><ul><li>20 to 50-fold enhanced adenovirus-mediated gene expression
    12. 12. Increased gene transfer in vivo
    13. 13. Accessibility of cell types </li></ul> MOI 200 MOI 200<br />NIH-3T3 cells were transduced with an Adenovirus Serotype 5 expressing EGFP with a MOI of 200 without and in the presence of TransMax. <br />
    14. 14. Our expertise: Cell technology<br /><ul><li>High performance shRNAs with >90% knockdown efficiency
    15. 15. Inducible knockdown via partnership with TET systems</li></ul>shRNA design and <br />cloning into Sirion screening system<br />Determination of<br />knockdown efficiency by QPCR<br />Confirmation of knockdown <br />on the protein level<br />by Western blot<br />Ad368-high<br />performance<br /> shRNA<br />>90% KD<br />
    16. 16. >90% knockdown – Guaranteed! <br />Shorter Timelines and<br />Improved Quality<br />High performance viral vectors with guaranteed >90% gene knockdown<br />Stable or inducible knockdown cells for reliable results<br />Short timelines save money compared to long in house development <br />Sirion’s experienced team can quickly generate the cell model you need <br />
    17. 17. More predictive screening<br />Primary cell line generation<br /><ul><li>Permanent Human Primary Cell immortalization
    18. 18. Polyclonal or monoclonal cell line generation
    19. 19. Phenotypic and functional characterization</li></ul>Conditional primary cell immortalization<br /><ul><li>The immortalizing gene is active at 33°C / inactive at 37°C
    20. 20. Conditionally immortalized cells terminally differentiate with close to primary cell type phenotype and function for more reliable results.</li></ul>* hTERT requires a license from Geron Inc.<br />
    21. 21. Validated frozen cells direct to your lab<br /><ul><li>Validated gene knock-down or over expression
    22. 22. Guaranteed cell viability >90%
    23. 23. Standardized product (cell passage number, cell physiology, gene expression / knock-down) </li></ul>Applications<br /><ul><li>Infectious disease research
    24. 24. Drug candidate screening
    25. 25. Target validation in vivo (tumor models)
    26. 26. Production cell line optimization</li></li></ul><li>Our expertise: Cell line optimization<br />Optimization of production cell lines for vaccines and antibodies <br />Target Gene<br />Identification<br />Mission® <br />Cells<br />Functional<br />Testing<br />Production<br />Cell Line <br />Target<br /> Validation<br />Target <br />Identification<br />Cellular<br /> Assays<br />Optimized<br />Production cell<br /><ul><li>Rational choiceof target genes by dynamic mRNA profiling
    27. 27. Identification ofcritical cellular pathways for productivity
    28. 28. Over-expression or knockdown of target genes
    29. 29. Stable genetic modification
    30. 30. Antibody yield (ELISA testing)
    31. 31. Virus yield (PFU/IU testing)
    32. 32. Cellular assays /enzyme activity
    33. 33. Culture medium optimization
    34. 34. Genetic cell modification
    35. 35. Gene Silencing</li></li></ul><li>Sirion Delivers<br />Shorter Timelines and<br />Improved Quality<br />Functionally tested, ready-to-use tailor-made genetically modified cells, <br />Guaranteed gene delivery into virtually any mammalian cell type<br />Guaranteed >90% knockdown maintained over the required experimental time frame<br />Results in weeks instead of the months needed for in-house solutions<br />
    36. 36. Experts in Gene Silencing and Over-expression<br />Delivering ANY Gene to ANY Cell<br />SIRION BIOTECH GmbH Tel: +49 89 700 961 999Am Klopferspitz 19 Fax: +49 89 700 961 99882152 Martinsried info@sirion-biotech.comGermany www.sirion-biotech.com<br />

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