Paper review 전상귀 20130817

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  • 계면활성제는 양친매성 저독성 생분해성 여러 ph 에서 안전성등이 장점 소포는 표면장력을 억제 유화력은 항균력등으로 이득을 나타냄 소포는 석융래 계면활성제를 대체할 수 있으며 여러 산업에 이요가능함
  • Lauryl alcohol 은 계면활성제의 중요한 성분 탄소수 12 향수에도 쓰임
  • 20 이상은 저해시킴 성장곡선을 저해시키다는데 무슨이유인지는 안밝힘
  • Fourier transform infrared at 3,391.53 cm-1 corresponds to the O–-H masCH2)) and symmetrical stretching (msCH2) of methylene occur at 2,926 and 2,854.32 cm-1 Sugar C–-O stretch of C–-O–-H groups is at 1,048.19 cm-1 of C–-O band of the acetyl esters appears at 1,240.79 cm-1 1,624 cm-1 band is from the stretching of the unsaturated C–-C of C–-O band of C (-O)-O–-C in lactones appears at 1,157 cm-1
  • 유화인덱스는 활동에 비례한다 유화력 – 물 기름 섞는것
  • 젖음 속도 캔버스 디스크 방법 젖으면 물에 가라앉는데 필요한 시간 .
  • 높은 접촉 각은 낮은 표면 에너지 또는 화학친화력을 나타낸다 .
  • 테이블 세로 0 2 4 6 은 배양시간 그옆이 세포 생존률
  • 물리화학적 다른 성질 유화력 표면장력 항균력등이 나타남 .
  • Paper review 전상귀 20130817

    1. 1. paper review 전상귀 Objective : demonstrated the use of lauryl alcohol as a potential lipidic feed stock for sophorolipid production using C. bombicola.
    2. 2. Introduction Biosurfactants, alternatives to chemically synthesized surfactants, have garnered increasing attention. biodegradability, low toxicity and resistance to temperature and pH variations Sophorolipids exhibit surface-active and emulsification properties, antimicrobial activities. Sophorolipid show anticancer activity, and are recommended for the treatment of skin diseases.
    3. 3. Material and method Chemicals - Lauryl alcohol (C12–-14) Stain – Candida bombicola ATCC 22214 Effect of Glucose Concentration, Temperature Separation - centrifugation at 5000 rpm, 20min, Etyhl acetate Structural Characterization - Fourier transform infrared Surface tension Emulsification Activity Wetting Property Antibacterial Study
    4. 4. Result Effect of Time Incubation period in the range of 24– 144 h at 30 C at 180 rpm. Increase in sophorolipid yield was observed with varying time intervals , Maximum production was obtained at 96 h.
    5. 5. Result Effect of Carbon Source Range of 5–12 % Maximum production of SL was obtained when 10 % glucose concentration
    6. 6. Result Effect of initial pH Maximum production of SL was obtained when the initial pH of the medium was 6.0.
    7. 7. Result Effect of Temperature Range 25–35 30 C resulting in good yield of the product Metal ions is hamper the stationary phase of the organism
    8. 8. Results Effect of Metal ions Range of 10–30 mM. Maximum ceoncentration 20 mM of Fe2+ and Zn2+ Ions
    9. 9. Results Structural Characterization of the Produced SLLA by FTIR From the following FTIR data it could be concluded that alkylsophorosides/ sophorolipid must have formed and hence showing good surface activities.
    10. 10. Results Emulsification Activity SLLA showed good emulsifying activity Emulsification activity was stable for the above-mentioned emulsions for more than 2 weeks. As per the formula mentioned, the emulsification index was calculated for different surfactants
    11. 11. Results Surface Tension Surface tension of the crude sophorolipid was investigated in comparison with that of SDS, laureth 4 and SLOA. . SLLA reduced the surface tension of distilled water to 24 mN/m with a CMC value of 0.68 mg/L.
    12. 12. Results Wetting Property Concentration increases, the wetting time decreases wetting power of the surfactants increases with increasing concentration.
    13. 13. Results Contact Angle Waterproofing or water repellency is an important industrial process, which depends on contact angle values. A high contact angle indicates a low solid surface energy or chemical affinity
    14. 14. Results Antibacterial Activity Sophorolipids have a good inhibition property against gram positive bacteria but so far a very few reports show a satisfactory inhibition against gram negative bacteria. It was observed that E. coli showed zero percent cell survival when sophorolipid concentration was 3 mg with an incubation time of 2 h.
    15. 15. Results Antibacterial Activity In the case of S. aureus, a sophorolipid concentration of 0.6 mg and incubation time of 4 h resulted in zero percent cell survival SLLA showed better antibacterial ability than lauryl alcohol.
    16. 16. Conclusions Modification of the lipophilic portion of the sophorolipid structure has shown to alter the physicochemical properties. These sophorolipids were found to have higher hydrophobicity and good emulsification activity. Surface tension reduction ability was very high compared to synthetic surfactants along with low CMC values. Sophorolipids using lauryl alcohol have potent antimicrobial activity on both gram negative bacteria, E. coli and gram positive bacteria, S. aureus.

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