Intermediary metabolism ppt BIOCHEMISTRY vkunder637@gmail.com

1,242 views
1,084 views

Published on

0 Comments
1 Like
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total views
1,242
On SlideShare
0
From Embeds
0
Number of Embeds
1
Actions
Shares
0
Downloads
82
Comments
0
Likes
1
Embeds 0
No embeds

No notes for slide
  • If rat liver slices are incubated with medium containing glucose , co2 is evolved.Of the systems presented here, precision-cut liver slices resemble most closely the in vivo situation, which contains not only the enzymes of the whole liver but also the connections between individual cells. The technique of maintaining liver slices is demanding. The thickness of a slice has to be minimal within the limits of the optimal number of cell layers and oxygen and nutrient transportation (Vickers et al. 1995). Recently, methods for cryopreservation of tissue slices have been developed (for example Fisher et al 1993; Ekins et al. 1996; de Kanter et al. 1998; Glöckner et al. 1998; de Graaf et al.2000). These methods allow more flexible use of slices, because viability and CYP activities seem to be preserved quite well (de Graaf et al. 2000; Renwick et al. 2000).
  • Isotopes Specifically Label Molecules withoutAltering Their Chemical Properties
  • Isotopes Specifically Label Molecules withoutAltering Their Chemical Properties
  • Intermediary metabolism ppt BIOCHEMISTRY vkunder637@gmail.com

    1. 1. Dr Vijay Marakala Biochemistry SIMS & RC 8/16/2013 2:47 AM
    2. 2. Metabolism Sum of all the chemical transformations taking place in a cell or organism, occurs through a series of enzyme- catalyzed reactions that constitute metabolic pathways. 8/16/2013 2:47 AM
    3. 3. 8/16/2013 2:47 AM
    4. 4. Precursor Intermediates Product Metabolite s 8/16/2013 2:47 AM
    5. 5. Intermediary metabolism Combined activities of all the metabolic pathways that interconvert precursors, metabolites, and products 8/16/2013 2:47 AM
    6. 6. Catabolism Anabolism 8/16/2013 2:47 AM
    7. 7. Catabolism Anabolism
    8. 8. 8/16/2013 2:47 AM
    9. 9. 8/16/2013 2:47 AM
    10. 10. 8/16/2013 2:47 AM
    11. 11. Methods of study of Intermediary metabolism Use of whole organisms or its components Use of metabolic probes Use of isotopes 8/16/2013 2:47 AM
    12. 12. Use of whole organism or its components Whole organism Isolated organs Organ slices Intact cells 8/16/2013 2:47 AM
    13. 13. Methods of study of Intermediary metabolism Use of whole organisms or its components Use of metabolic probes Use of isotopes 8/16/2013 2:47 AM
    14. 14. Utility of metabolic probes Inhibitors Mutants 8/16/2013 2:47 AM

    ×