- -
Dr. Vijay Marakala M.D
Biochemistry
SIMS&RC
CONTENTS
Central Dogma
Mechanisms
Basic requirements
Stages
Inhibitors
- -
RNA
PROTEIN
DNA
Central
Dogma
- -
- -
DNA
structure
- -
Semi-
conservative
mechanism
- -
- -
- -
Basic
requirements
Requirements
Substrates
Template
Enzymes
Primer
Requirements
- -
Basic
requirements
Substrates
• dATP
dGTP
dCTP
dTTP
Requirements
- -
Basic
requirements
TEMPLATE
- -
ENZYMES
DNA A Protein
DNA Helicase
Primase
DNA Polymerase
SSB
DNA Topoisomerase
DNA Ligase
Ter binding protein
Enzymes...
STAGES
Initiation
Elongation
Termination
- -
Unwinding
and formation
of replication
fork
- -
Replication bubble
- -
DNA
Replication
is
Bidirectional
- -
Helicase
unwinds
DNA
- -
5’
3’
5’
Primase synthesizes PRIMER
Single stranded binding protein
- -
5’
5’
3’
3’
5’
3’
Parental DNA
Leading strand
Laging strand
5’
3’
5’
Okazaki fragments
Primer
Gap filled byDNA Ligase
...
- -
- -
- -
DNA
Ligase
- -
Termination
sequences
“Ter” direct
termination
- -
- -
DNA →DNA
Bidirectional
Primers
• Semiconservative
mechanism
• Begins at origin
• Synthesis5→3’
• Read 3’→5’
• Leading stra...
Differences between prokaryotic
and eukaryotic DNA replication
- -
Features Prokaryotes Eukaryotes
RNA primer length 50 nu...
- -
- -
- -
Nalidixic acid
Novobiocin
Ciprofloxacin
- -
Adriyamycin
Etoposide
Doxorubicin
- -
THANK YOU
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DNA replication ppt BIOCHEMISTRY

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DNA replication ppt BIOCHEMISTRY

  1. 1. - - Dr. Vijay Marakala M.D Biochemistry SIMS&RC
  2. 2. CONTENTS Central Dogma Mechanisms Basic requirements Stages Inhibitors - -
  3. 3. RNA PROTEIN DNA Central Dogma - -
  4. 4. - -
  5. 5. DNA structure - -
  6. 6. Semi- conservative mechanism - -
  7. 7. - -
  8. 8. - - Basic requirements Requirements Substrates Template Enzymes Primer
  9. 9. Requirements - - Basic requirements Substrates • dATP dGTP dCTP dTTP
  10. 10. Requirements - - Basic requirements TEMPLATE
  11. 11. - - ENZYMES DNA A Protein DNA Helicase Primase DNA Polymerase SSB DNA Topoisomerase DNA Ligase Ter binding protein Enzymes and Proteins
  12. 12. STAGES Initiation Elongation Termination - -
  13. 13. Unwinding and formation of replication fork - - Replication bubble
  14. 14. - - DNA Replication is Bidirectional
  15. 15. - - Helicase unwinds DNA
  16. 16. - - 5’ 3’ 5’ Primase synthesizes PRIMER Single stranded binding protein
  17. 17. - - 5’ 5’ 3’ 3’ 5’ 3’ Parental DNA Leading strand Laging strand 5’ 3’ 5’ Okazaki fragments Primer Gap filled byDNA Ligase Elongation by DNA Polymerase IIIPrimer is removed by DNA Polymerase I
  18. 18. - -
  19. 19. - -
  20. 20. - -
  21. 21. DNA Ligase - -
  22. 22. Termination sequences “Ter” direct termination - -
  23. 23. - -
  24. 24. DNA →DNA Bidirectional Primers • Semiconservative mechanism • Begins at origin • Synthesis5→3’ • Read 3’→5’ • Leading strand • Laging strand - -
  25. 25. Differences between prokaryotic and eukaryotic DNA replication - - Features Prokaryotes Eukaryotes RNA primer length 50 nucleotides 9 nucleotides DNA polymerase I,II,III α,β,γ,δ,ε Number of origins Single Multiple Nucleotide length of Okazaki 1000-2000 nucleotides 200 nucleotides Rate of replication 500 nucleotides/sec 50 nucleotides/sec
  26. 26. - -
  27. 27. - -
  28. 28. - - Nalidixic acid Novobiocin Ciprofloxacin
  29. 29. - - Adriyamycin Etoposide Doxorubicin
  30. 30. - - THANK YOU
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