Catarina Duarte / Design of nutraceuticals targeting cancer
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Presentation given by Catarina Duarte from the Chemistry and Biology Technological Institute (New University of Lisbon) in the framework of the Emergence Forum Barcelona ...

Presentation given by Catarina Duarte from the Chemistry and Biology Technological Institute (New University of Lisbon) in the framework of the Emergence Forum Barcelona

Biocat organized the Barcelona Emergence Forum (April 10-11th, 2014, Congress Palace, Montjuïc) supported by the TRANSBIO SUDOE, a translational cooperation project dedicated to innovation in life sciences in South-West Europe. The Barcelona Emergence Forum contributed to bringing together Academics, Companies, Investment Entities, Technology Platforms and Technology Transfer Offices from Spain, France and Portugal to set up collaborative projects on Human Health & Agro-food Innovation.
More information at: http://www.b2match.eu/emergenceforum2014

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Catarina Duarte / Design of nutraceuticals targeting cancer Catarina Duarte / Design of nutraceuticals targeting cancer Presentation Transcript

  • Development of Nutraceuticals targeting cancer Catarina Duarte Food & Health Unit Nutraceuticals and controlled Delivery Lab IBET/ ITQB-UNL Oeiras, Portugal
  • Green Processes for developing health promoting products Extraction /Fraccionation of Natural Compounds Research interests: Formulation – particle formation, encapsulation and impregnation Characterization and pre-clinical Bioactivity evaluation of bioproducts Chemical, enzymatic and cell-based assays Food&Health Unit Nutraceuticals and Controlled Delivery Lab
  • POH was detected in 10%EtOH and 20%EtOH extracts Serra et al. (2010) J Supercri. Fluids, 55, 184-191 Sweet cherries Development of POH-rich extracts Application of High Pressure Technology for the development of natural extracts rich in perillyl alcohol (POH) Raw material: Sweet cherry culls (Saco variety) POH
  • 96h Only two extracts (10%EtOH and 20%EtOH) inhibited cancer cell growth in a time dependent manner and this effect is related with the presence of Perillyl Alcohol Cherry extracts induced cell cycle arrest in a different cell cycle checkpoint than doxorrubicin suggesting that they can be used in combination with the drug in chemotherapy 0 20 40 60 80 100 Control 4h 24h 48h 72h 96h cell(%) G1 S G2/M Cherry extract- 10% EtOH 0 20 40 60 80 100 Control 4h 24h 48h 72h 96h cell(%) G1 S G2/M Doxorubicina 0 20 40 60 80 100 120 Cellviability(%ofcontrol) 24h 48h 72h 96h 10% EtOH 20% EtOH 40% EtOH 60% EtOH 80% EtOH 100% EtOH CO2 Antiproliferative effect on HT29 cells (human colorectal cancer cell line) • Cell cycle analysis Controlo 10% EtOH 20% EtOH • Cell viability after 24, 48, 72 and 96h of proliferation Serra et al. (2010) J Supercrit. Fluids, 55, 184-191 Serra et al. (2011) J Supercrit. Fluids,55 1007-1013 1st prize: “Inovation and Develo+ment of Products and Services” category Sweet cherries Development of POH-rich extracts
  • Minutes 0 10 20 30 40 50 60 70 80 90 100 110 120 130 0 500 1000 1500 2000 2500 3000 3500 4000 4500 0 500 1000 1500 2000 2500 3000 3500 4000 4500 Detector 1-280nm Laranja (2) TS Ago2913075.dat Detector 1-280nm Laranja (3) TS Ago2913076.dat Detector 1-280nm Laranja (4) TS Ago2913077.dat Detector 1-280nm Laranja Dil 1:10 Jul2413094.dat Sinensetin Nobiletin Tangeretin Process: CO2+ EtOH (80:20 v/v); P=25MPa; T=323K; t=60min. Tangeretina Nobiletin Sinensetin Inês Silva (2013), Tese de mestrado Citrus peels Development of PMF-rich extracts Application of Supercritical Fluid Technology for the development of natural extracts rich in polymetoxylated flavones (PMF) Sinensetin, tangeritin and nobiletin were identified in citrus pell extracts. Raw material: Orange peels (by-products of fruit juice production)
  • Induction of apoptosis on HT29 cell spheroids Control Citrus extract (Red: viable cells; Green: apoptotic cells) Cell viability • 2D model Orange extract inhibited the proliferation of HT29 cells (antiproliferative effect was dependent on spheroid size). Orange extract induced apoptosis in HT29 spheroids(apoptotic cells are mainly located on the surface of the spheroid). • 3D model Cell monolayer Cell spheroids with different sizes Antiproliferative effect on HT29 cells (human colorectal cancer cell line) Inês Silva (2013), Master thesis Citrus peels Development of PMF-rich extracts
  • Opuntia ficus-indica Development of terpene-rich extracts Raw material: Opuntia ficus-indica (by-products of fruit juice production) Process: CO2+ EtOH+ H2O mixtures; P=20MPa; T=313K; t=180min. Application of Pressurized Liquid Extracton for the development of natural extracts rich in terpenes fried lup #1 #2 #3 #4 #5 #6 #7 #8 #9 #10fried lup #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 Terpene detection by TLC Opuntia extracts obtained with mixtures of CO2 and EtOH presented high terpene content
  • Opuntia extract obtained with CO2:EtOH (60:40v/v) mixture (PLE2) presented the highest antiproliferative effect. This effect is probably due to the presence of terpenes. PLE-2 extracts increased drug sensitivity on HT29 dx cell population cells . Antiproliferative effect on HT29 cells (human colorectal cancer cell line) • Cell viability after 24 h of proliferation 0 20 40 60 80 100 1 2 3 4 5 6 7 8 9 10 Cellularviability 16 mg/mL 20 mg/mL 24 mg/mL 0 10 20 30 40 50 60 70 80 90 100 110 0 5 10 15 20 Cellviability(%ofcontrol) Doxorubicin (µM) Doxorubicin in HT29 dx Doxorubicin in HT29 PLE-2 + Doxo in HT29 dx • Chemosensitization effect in a drug resistant cell sub- population (HT29 dx) Joana Poejo(2014), Master thesis Opuntia ficus-indica Development of terpene-rich extracts
  • e-mail: cduarte@itqb.unl.pt; http://www.itqb.unl.pt/labs/nutraceuticals-and-delivery www.ibet.pt Laboratório de Nutracêuticos e Libertação Controlada Oeiras, Portugal