Sonia Da Rochas / Novaptech, biotechnological development of aptamers

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Presentation given by Sonia Da Rochas from Novaptech, in the framework of the Emergence Forum Barcelona

Biocat organized the Barcelona Emergence Forum (April 10-11th, 2014, Congress Palace, Montjuïc) supported by the TRANSBIO SUDOE, a translational cooperation project dedicated to innovation in life sciences in South-West Europe. The Barcelona Emergence Forum contributed to bringing together Academics, Companies, Investment Entities, Technology Platforms and Technology Transfer Offices from Spain, France and Portugal to set up collaborative projects on Human Health & Agro-food Innovation.
More information at: http://www.b2match.eu/emergenceforum2014

Published in: Healthcare, Technology, Business
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Sonia Da Rochas / Novaptech, biotechnological development of aptamers

  1. 1. Biotechnological development of Aptamers Dr. Sonia Da Rocha Gomes Emilie Daguerre Laetitia Evadé-Farrugia Novaptech, 146 rue Léo Saignat, bat 3A, BP12 Bordeaux, France sonia.darocha@novaptech.com +33.5.57.57.95.05 10th April 2014, Human Health Track, Barcelona
  2. 2. Novaptech, business unit -Created in 2008, -3 people, Bordeaux -Dedicated to the biotechnological development of APTAMERS -Managed by the association -Connected to the academic lab Inserm U869, Dr. Toulmé ’s team > 15 years of expertise in aptamer field (11th, Agrofood session) - Two automated platforms of selection and aptamer production, unique in France Aptamer production - Identification - Optimization - Conjugation Aptamer-based tools in analytical, diagnostic or therapeutic fields Method development -Implementation of new strategies -Improvement of the automated platforms OFFER : Aptamer selection against your target and characterization Service contracts with academic labs and companieS  30 projects
  3. 3. Your needs, looking for new molecular probe DETECTION AND MONITORING (molecular imaging, biosensors, sandwich assay) PURIFICATION (affinity capture, sorting) CHARACTERIZATION (target validation, protein-binding RNA sites) INHIBITION (drug delivery, drug discovery)REGULATION (artificial riboswitch) Aptamers, the right solution
  4. 4. Aptamer properties, Structured oligonucleotides identified:  from randomly synthesized library  against a target  through combinatorial strategy (SELEX) iterative rounds of selection / amplification (Tuerk and Gold,1990; Ellington and Szostak, 1990)  High affinity and specificity  Rival antibodies  Chemical synthesis  Chemically modified and conjugated to numerous pending groups (fluorophore, radioelements, biotin..)
  5. 5. Aptamer selection process, 146 rue Léo Saignat, bat 3A, BP12 Bordeaux, France sonia.darocha@novaptech.com +33.5.57.57.95.05 Phase 1 Phase 2 Automated platform for aptamer selection, unique in France (Inserm U869 lab) Aptamer Selection Screening (Affinity) Cloning Sequencing, Sequence analysis Candidates production and binding validation Optimization - Minimizing their size - Improving nuclease resistance - Conjugation: biotine, amine, fluorochrome..

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