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Putting Biomanufacturing                Putting Biomanufacturing                 into Your CurriculumSonia WallmanExecutiv...
NBC2 Helps                                  pLearning Communities   Curriculum          Goal 1:  Build and Foster Local Pa...
Enhance, Expand and Use the NBC2 GBC E h      E     d d U thCurriculum in Response to Industry Needs
Global BioManufacturing Curriculum             Virtual Labs
NBC2 Biomanufacturing         Curricular Components        Curricular ComponentsGBC Laboratory Manual generic and customiz...
Introduction to Biomanufacturing             Textbook            T tb k               • A th d b i d t                 Aut...
Introduction to Biomanufacturing    Introduction to BiomanufacturingEngineering                  Quality                  ...
Global Biomanufacturing Curriculum Biomanufacturing Laboratory Manual                 Laboratory Manual                   ...
Biofuels Curricullum
GBC Biomanufacturing       Laboratory Manual Ad      L b        M    l Adopters• Mount  Wachusett Community College, MA (B...
Biofuels Production and Analysis             Textbook            T tb k                •   Introduction                   ...
Biofuels Laboratory Manual   f              y             • Introduction to Biofuels                Production            ...
NBC2 Biofuels CurriculumPart of the EDGE (Educating and Developing New P     f h EDGE (Ed        i     dD l i NWorkers for...
Protein is Cash Teachers Workshop Laboratory ManualT h W kh L b t               M    l   Day One:  Metrology   Day Two:  T...
Protein is Cash Workshops                                         p2010                       2011               2012Biote...
Adult Biomanufacturing                Certificate Program               Certificate Program           Derived from Protein...
Adult Biomanufacturing Certificate Program                        DetailsWeek     Lecture                                 ...
Week   Lecture                                 Laboratory Work5      Downstream Processing g                 Homogenizatio...
The Virtual   Chromatography Laboratory   Chromatography Laboratory   Virtual Laboratory Scale    Virtual Laboratory Scale...
Virtual Chromatography –     The Power of Interactive Visualization in       Understanding a STEM Fi ld f S d      U d    ...
Actual BioLogic System                      g y• Complex System• Not easy to ‘see’   interaction of components• Students u...
Chromatography Skid – Components  g       g                      gyEngineering and Advanced Technology     A screenshot of...
Chromatography Skid – Controller           g       g         Engineering and Advanced TechnologygyThe Virtual Liquid Chrom...
Chromatography Skid –             Chromatogram with Mathematics             Chromatogram with MathematicsThe Virtual Chrom...
Chromatography Skid –                    g p y                      gy            Chromatography Science and TechnologyThe...
Chromatography Skid –                   g p y                      gy           Chromatography Science and TechnologyThe V...
Downstream Processing Equipment Similarities         Lab Scale            Industrial Scale  Chromatography System Ch    t ...
The Virtual Chromatography          Laboratory         L b tUsed at MCCC, GBCC, MWCC, and FLCCUsed at MCCC GBCC MWCC and F...
Virtual Biopharmaceutical Production           p            NBC2 in partnership with    Yakov Cherner of ATe Learning, Inc...
Virtual BiomanufacturingFor previews of NBC2’s virtual, industrial‐scale biomanufacturing facility and    virtual laborato...
Disseminate Biomanufacturing Education andTraining,Training Workforce Statistics and Career Pathways           www.biomanu...
Month        Sites    KBytes    Visits   Pages   Files   Hits       yFebruary 2010   5137    32936942   6254     12063   2...
Build and Foster Local Partnerships Amongst   Industry, Education and Organizations
Community College ‐ Biomanufacturer Partner:  Mount Wachusett Community College   Mount Wachusett Community College ‐ BMS ...
Community College ‐ Biomanufacturer Partner: NH Community Technical College (GBCC) ‐ LonzaLonza (earlier, Celltech Biologi...
Mentored Community College   Biomanufacturing Programs  Bi     f t i PMt. Wachusett Community College, MAQuincy College, M...
National Regional Alliances            gMid-Western AllianceMid W t      AlliIvy Tech Community College,Bloomington, INBl ...
Provide Professional Development for Provide Professional Development for   Faculty, Students and Teachers 
BIOMAN 2010 Annual Conference              July 12‐15, 2010      Ivy Tech Commmunity College           Bloomington, Indian...
BIOMAN 2011                             MiraCosta College, Oceanside, CA                                     July 11‐14, 2...
BIOMAN 2012                          July 23‐26, 2012                   Alamance Community College                   Alama...
Sustainability
Plan for NBC2 Sustainability Sale of Biomanufacturing Curriculum Materials Sale of Biomanufacturing Curriculum Materials‐ ...
How to integrate biomanufacturing workforce development into your biotechnology curriculumdevelopment into your biotechnol...
Biomanufacturing Course            Development           D l         tChoose existing textbooks and laboratory manuals or ...
Individual Curriculum ModulesOver 75 biopharmaceutical biomanufacturing SOPs based on industry practices.11 biopharmaceuti...
Course in a BoxThe NBC2 plan is to provide a ‘Course in a Box’ type of format to deliver the materials necessary       ff ...
Protein is Cash Course in a Box     Protein is Cash ‐ Day 3: Downstream Processing     Item                          Sourc...
Cellulosic Biofuels Course in a Box  Biofuels Laboratory 2:  Using Cellulosic Enzymes to Produce Bioethanol             It...
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
Presentation6 bioman course in a box wallman
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Presentation6 bioman course in a box wallman

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Presentation by Dr. Sonia Wallman at Southern California Biotechnology Conference

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Transcript of "Presentation6 bioman course in a box wallman "

  1. 1. Putting Biomanufacturing  Putting Biomanufacturing into Your CurriculumSonia WallmanExecutive Director, NBC2January 13, 2012 – Bio‐Link Conference
  2. 2. NBC2 Helps pLearning Communities Curriculum Goal 1:  Build and Foster Local Partnerships          G l 1 B ild d F L lP hi Between Biomanufacturers, Educational  Professional Institutions and Organizations Development p Goal 2: Enhance Expand and Use the NBC2 Goal 2:  Enhance, Expand and Use the NBC Global Biomanufacturing Curriculum in  Dissemination Response to Biomanufacturers Needs Goal 3:  Provide Professional Development  Goal 3: Provide Professional DevelopmentCapacity Building ld Opportunities in Biomanufacturing for  Faculty, Teachers and Students SUSTAINABILITY Goal 4:  Disseminate Information on  Biomanufacturing Education and Training,  Workforce Statistics and Career  Opportunities Goal 5:  Plan for Sustainability 
  3. 3. Enhance, Expand and Use the NBC2 GBC E h E d d U thCurriculum in Response to Industry Needs
  4. 4. Global BioManufacturing Curriculum Virtual Labs
  5. 5. NBC2 Biomanufacturing  Curricular Components Curricular ComponentsGBC Laboratory Manual generic and customizedGBC L b t M l i d t i dIntroduction to Biomanufacturing textbookBiofuels Production and Analysis textbook and lab manualProtein is Cash Teachers WorkshopBIOMAN Online Adult Certificate/Incumbent BIOMAN Online Adult Certificate/IncumbentWorker biopharmaceutical biomanufacturing course Future = more customization of curriculum to suit F t t i ti f i l t itlocal biomanufacturing needs
  6. 6. Introduction to Biomanufacturing  Textbook T tb k • A th d b i d t Authored by industry experts  t • 11 chapters plus introduction • Industry using textbook for Industry using textbook for   incumbent technician training • Scheduled for release, spring Scheduled for release, spring  2012
  7. 7. Introduction to Biomanufacturing Introduction to BiomanufacturingEngineering Quality Production• Operational  • Quality Assurance • Upstream Processing Cynthia Sarnoski,  Cynthia Sarnoski, Excellence E ll Frank Hallinan, Pfizer Olga Mollin, Lonza l lliMarti Mathews, Biogen Idec Ron Hudcosky, Merck Jane Flanagan, Lonza• Facilities • Downstream Brian Shingle, Centocor • Microbial Control Processing John Hasyn, MCCC• Metrology Paul O’Neil, Pall EuroflowGene Kernan, HGSIGene Kernan HGSI • Process Development Process Development • QC Biochemistry• Validation Mary Harne,  HGSI Ezster Birck WilsonPatrick Sheehy, HGSI Zhenhong Lee., HGSI• EH&SJohn Piampiano, Kodak
  8. 8. Global Biomanufacturing Curriculum Biomanufacturing Laboratory Manual Laboratory Manual  C Metrology: SOPs for equipment Validation Protocols EH&S Program Quality Assurance:  cGMP, SOPs, Batch  Records, Equipment Logs d Quality Control Microbiology SOPs Upstream Processing:  E. coli recombinant for GFP,  E coli recombinant for GFP CHO recombinant for tPA, Pichia pastoris recombinant for HSA Downstream Processing:  Downstream Processing: Purification of  tPA from CHO Cell Culture Purification of HSA from Pichia pastoris Cell  Culture Quality Control Biochemistry:  SDS‐PAGE, tPA  and HSA ELISAs
  9. 9. Biofuels Curricullum
  10. 10. GBC Biomanufacturing  Laboratory Manual Ad L b M l Adopters• Mount  Wachusett Community College, MA (BMS is  y g ( biomanufacturer partner)• Minuteman Regional High School Adult Evening  Program, MA (Shire is biomanufacturer partner) ( )• Hudson Valley Community College, NY (Regeneron is  biomanufacturer partner) biomanufacturer partner)• Great Bay Community College, NH (Lonza is  biomanufacturer partner) p )• Montgomery and Bucks County Community Colleges, PA• Hagerstown Community College, MD• Community Colleges, southern CA
  11. 11. Biofuels Production and Analysis  Textbook T tb k • Introduction I t d ti • Energy • Fuels F l • Gases • Molecules & Biomass • Enzymes • Photosynthesis and Algae • Biofuels
  12. 12. Biofuels Laboratory Manual f y • Introduction to Biofuels  Production • Using Cellulosic  Enzymes to Produce  y Bioethanol • Using Bacteria to  Produce Biohydrogen • Using Algae to Produce  Biodiesel
  13. 13. NBC2 Biofuels CurriculumPart of the EDGE (Educating and Developing New P f h EDGE (Ed i dD l i NWorkers for the Green Economy) program in San Diego.DiegoOne of several certificate courses offered at community colleges and universities in San Diegocommunity colleges and universities in San DiegoPiloted at MiraCosta College in 2011 and University of Hawaii  Hilo in 2012.of Hawaii – Hilo in 2012.Support site under development at www.biomanonline.com
  14. 14. Protein is Cash Teachers Workshop Laboratory ManualT h W kh L b t M l Day One:  Metrology Day Two:  Transformation and Upstream Processing Day Two: Transformation and Upstream Processing Day Three:  Downstream Processing Day Four:  Quality Control Biochemistry Day Four: Quality Control Biochemistry Day Five:  Cellulosic Biofuels Support site at: www.biomanonline.org S t it t bi li
  15. 15. Protein is Cash Workshops p2010 2011 2012Biotechnology Center URIBiotechnology Center URI Hillsborough CC Hillsborough CC Windward CC Windward CCProvidence, RI Brandon, FL Kaneohe, HIHudson Valley CC Hudson Valley CC Mount Wachusett CCAlbany, NYAlbany, NY Troy, NY Devens, MA Devens, MAMinuteman HS Houston CC LA Trade TechLexington, MA Houston, Texas Los Angeles, CAHillsborough CC Middlesex CC Middlesex CCTampa, FL Edison, NJ Edison, NJMesa CC Blue Grass CTC Middlesex CCMesa, AZ Lexington, KY Lowell, MAGreat Bay CC Middlesex CC Austin CCPortsmouth, NH Lowell, MA Austin, TX
  16. 16. Adult Biomanufacturing  Certificate Program Certificate Program Derived from Protein is Cash and the Global Biomanufacturing Curriculum • 12 week course comes with online lectures• 6 hours of hands‐on activities with lecture            6 hours of hands‐on activities with lecture per day• Total of 360 hours for Biomanufacturing Certificate• Focuses on putting people to work immediately • Version piloted at global bioman company in 2009
  17. 17. Adult Biomanufacturing Certificate Program DetailsWeek Lecture Laboratory Work  y1 Intro to Biotech/Biomanufacturing & GMP Documentation of work: GMP activity Metrology : Intro to precision  and accuracy Balances, micropipets , pp2 Upstream Processing: Basic Cell Structure Sterile techniques Microbes versus animal cells  Media preparation/ pouring  Cell Growth plates Antibiotic sensitivity3 Upstream Processing and Genetic Transformation  Autoclave validation of  of DNA/ Protein Characteristics sterilization4 Upstream Processing : Scaling up of cells Determination of cell number Cell growth , crude isolation of product Use of spectrophotometer
  18. 18. Week Lecture Laboratory Work5 Downstream Processing g Homogenization and centrifugation : initial  g g Methods of Purification product separation   Media, Cell Concentration TFF concentration of product solution Crude Isolate 6 Solution Preparation and pH  Salt and pH effect on proteins in solution Determinations Use of the pH meter   Preparation of buffer solutions7 ‐8 Purification of product by  Size exclusion, hydrophobic interaction, Ion Chromatography Techniques exchange 9 Quality Control Analysis of Purity of Quality Control Analysis of Purity of PAGE analysis of product purity PAGE analysis of product purity Product ELISA /product identification10 Documentation of Products by Batch  Calculation of total yield of product Records, Final Formulation  Records Final Formulation FDA review process for Drug Approval FDA review process for Drug Approval FDA and Drug Approval11 Use of laboratory sized process  Growth of cells in bioreactor controlled bioreactor P.pastori s cell production of HSA
  19. 19. The Virtual  Chromatography Laboratory  Chromatography Laboratory Virtual Laboratory Scale  Virtual Laboratory Scale Chromatography NBC2 in partnership with in partnership with Yakov Cherner of ATe‐Learning, Incorporated
  20. 20. Virtual Chromatography – The Power of Interactive Visualization in  Understanding a STEM Fi ld f S d U d di STEM Field of StudyUnderstanding the physics chemistry and biology of the physics,chromatographic system and the binding of the protein ofinterest to the chromatographic matrix or beads (Science)Understanding the design and operation of chromatographycomponents and of the chromatographic process (Technologyand Engineering) Engineering).Understanding the calculations needed to run thechromatographic system (column volume) and themeasurements on chromatograms needed to calculate theHETP, number of theoretical plates, retention time, andresolution (M th l ti (Mathematics). ti )
  21. 21. Actual BioLogic System g y• Complex System• Not easy to ‘see’  interaction of components• Students use virtual  system to prepare to use  actual system•UUse virtual system for  it l t f BIOMANonline• System same as industrial System same as industrial  chromatography skid
  22. 22. Chromatography Skid – Components g g gyEngineering and Advanced Technology A screenshot of the Virtual Liquid Chromatography Laboratory. 3D images of major system components are delivered as you click on them.
  23. 23. Chromatography Skid – Controller g g Engineering and Advanced TechnologygyThe Virtual Liquid Chromatography Laboratory showing the interactive controller which enables students to operate the system and set process parameters. 
  24. 24. Chromatography Skid – Chromatogram with Mathematics Chromatogram with MathematicsThe Virtual Chromatography Laboratory teaches students how to make calculations on  chromatograms such as the efficiency of column packing (HETP). 
  25. 25. Chromatography Skid – g p y gy Chromatography Science and TechnologyThe Virtual Chromatography Laboratory showing the operation of the chromatography system during the‘load’ phase, the chromatogram showing the flow through of proteins that do not attach to thechromatographic matrix, and a nanoscale view inside the column of the affinity bead with the protein ofinterest in the filtrate (green) attached and proteins not specific for the bead flowing through the column.
  26. 26. Chromatography Skid – g p y gy Chromatography Science and TechnologyThe Virtual Chromatography Laboratory showing the operation of the chromatography systemduring h ‘elution’ hd i the ‘ l i ’ phase, the chromatogram showing the b i i of the peak of the protein h h h i h beginning f h k f h iof interest, and a nanoscale view inside the column of the affinity bead showing the protein ofinterest detaching from the bead as the elution buffer (red) moves through the column.
  27. 27. Downstream Processing Equipment Similarities Lab Scale  Industrial Scale  Chromatography System Ch t h S t Chromatography System Ch t h S t
  28. 28. The Virtual Chromatography  Laboratory L b tUsed at MCCC, GBCC, MWCC, and FLCCUsed at MCCC GBCC MWCC and FLCC
  29. 29. Virtual Biopharmaceutical Production p NBC2 in partnership with  Yakov Cherner of ATe Learning, Incorporated Yakov Cherner of ATe‐Learning Incorporated and Lonza Biologics, Portsmouth, NH
  30. 30. Virtual BiomanufacturingFor previews of NBC2’s virtual, industrial‐scale biomanufacturing facility and  virtual laboratory‐scale Bio‐Rad BioLogic LP chromatography system used  to practice and assess biomanufacturing skills see preview at  www.atelearning.com/BioDemo (use the password, Bio‐Rad).  /
  31. 31. Disseminate Biomanufacturing Education andTraining,Training Workforce Statistics and Career Pathways www.biomanufacturing.org www.biomanonline.org www.biomanufacturingcareers.org BIOMAN Group on LinkedIn
  32. 32. Month Sites KBytes Visits Pages Files Hits yFebruary 2010 5137 32936942 6254 12063 24653 47189February 2011 5621 77978037 4749 10908 37576 72294
  33. 33. Build and Foster Local Partnerships Amongst Industry, Education and Organizations
  34. 34. Community College ‐ Biomanufacturer Partner: Mount Wachusett Community College  Mount Wachusett Community College ‐ BMS http://www.nature.com/naturejobs/2009/090312/pdf/nj7235-246a.pdfSought NBC2 help from the time there was talk that a biopharmaceutical biomanufacturer was thinking of locating at Devens, MA (old military base).Campus engineer and architect of the proposed biotechnology/biomanufacturing laboratories to GBCC to view set‐up for biotech research, development and laboratories to GBCC to view set up for biotech research development andbiomanufacturing.Richard Elmer, Manager of Learning and Development at BMS , to GBCC to see biotech/biomanufacturing facility and discuss NBC2 biomanufacturing hands‐on curriculum.biotech/biomanufacturing facility and discuss NBC biomanufacturing hands on curriculum.Mount Wachusett adopted GBCC biotech/biomanufacturing facility set‐up and equipment.Once lab built, adopted NBC2 hands‐on biomanufacturing curriculum for first biomanufacturing class in Spring 2009.Mount Wachusett is customizing the NBC2 hands‐on laboratory curriculum for second biomanufacturing class this Spring 2010.NBC2 will publish the Mount Wachusett version of the NBC2 hands‐on laboratory curriculum at www.lulu.comMount Wachusett is helping NBC2 evaluate  the online Virtual Chromatography Laboratory curriculum to support the actual hands‐on NBC2 Chromatography SOPs.
  35. 35. Community College ‐ Biomanufacturer Partner: NH Community Technical College (GBCC) ‐ LonzaLonza (earlier, Celltech Biologics) provides help with devlopment of curriculum, including and o.j.t. in the apprenticeship program GBCC provides training in biotechnology and biomanufacturing in a  p o des a g b o ec o ogy a d b o a u ac u g abiotechnology associate in science degree and certificate programs in BiotechnologyGBCC provides skilled biomanufacturing technicians (in upstream and  p g ( pdownsteam processing, quality control microbiology and biochemistry, validation, process development, quality assurance) and upskilling of incumbent technicians for Lonza and others.  
  36. 36. Mentored Community College  Biomanufacturing Programs Bi f t i PMt. Wachusett Community College, MAQuincy College, MAHudson Valley Community College, NYHagerstown Community  College, MDOnondaga Community College, NYOnondaga Community College NYSoCal Biomanufacturing Consortium
  37. 37. National Regional Alliances gMid-Western AllianceMid W t AlliIvy Tech Community College,Bloomington, INBl i tWestern AllianceMira Costa CollegeOceanside, CASouthern AllianceAlamance CCGraham, NC
  38. 38. Provide Professional Development for Provide Professional Development for Faculty, Students and Teachers 
  39. 39. BIOMAN 2010 Annual Conference July 12‐15, 2010 Ivy Tech Commmunity College Bloomington, Indiana • 44 Participants • 4 High School teachers sponsored by NBC2 g p y • 15 Hands‐on Biomanufacturing workshops • Industry tours – Cook Pharmica, Baxter Biopharma • Vendor show Vendor show • Panel discussions • Keynote Speakers: ‐ Medical Devices Dr D Chadwick Cook Medical Medical Devices, Dr. D. Chadwick, Cook Medical ‐ Lyophilization in Biotechnology, Dr. M. Akers,            Baxter BioPharma Solutions ‐ Cell line Development in Biomanufacturing Dr A Cell line Development in Biomanufacturing, Dr. A.  Pilling, Cook Pharmica
  40. 40. BIOMAN 2011 MiraCosta College, Oceanside, CA July 11‐14, 2011Focus on biofuels and biopharmaceuticalsThree tracks for biomanufacturing hands‐on workshops:  advanced, Three tracks for biomanufacturing hands on workshops: advancedintermediate and beginnerKeynote Speakers include Stephen Mayfield, PhD, from UCSD and San Diego Center for Algae Biotechnology on  Microalgae for the Production of Biofuels Center for Algae Biotechnology on ‘Microalgae for the Production of Biofuelsand other Bioproducts’ and  Lee Landeen, PhD, from Advanced BioHealing on ‘Engineered Tissues for Wound Healing Applications’Tours of Genentech, Life Technologies (Invitrogen) and the Salk InstituteTours of Genentech Life Technologies (Invitrogen) and the Salk Institute
  41. 41. BIOMAN 2012 July 23‐26, 2012 Alamance Community College Alamance Community College Graham, North Carolina• Focus on regenerative medicine:  stem cell production, production of replacement organs• 13 Hands‐on Biomanufacturing workshops• Industry tours – BiogenIdec, Tengion, Wakeforest Regenerative Medicine Center• Vendor show• Panel discussions: How to Add Biomanufacturing to your Programs; Overview of NSF ATE and other Funding Opportunities for supporting Biomanufacturing Education, Training and the Workforce• Keynote Speakers: y p ‐ Regenerative Medicine, Dr. Anthony Atala,  WFIRM, Winston‐Salem, NC
  42. 42. Sustainability
  43. 43. Plan for NBC2 Sustainability Sale of Biomanufacturing Curriculum Materials Sale of Biomanufacturing Curriculum Materials‐ Textbooks and Laboratory Manuals‐ Lab Manuals Lab Manuals‐ Virtual Industrial and Lab Scale Biomanufacturing Modules        ‐ Materials to support Biomanufacturing Modules Annual Conference and Workshops Certification of Biomanufacturing Technicians 501C3 Approved by Administration at MCCC 501C3 A d b Ad i i i MCCC Incorporate – May/June 2012 Adopt corporate by‐laws Adopt corporate by laws Apply for non‐profit status (within 27 months)
  44. 44. How to integrate biomanufacturing workforce development into your biotechnology curriculumdevelopment into your biotechnology curriculum1. Partner with local biomanufacturers, educators at various  levels and organizations (e.g. US DOL).2. Develop program (sequence of liberal arts and STEM 2 D l ( f lib l d STEM courses=STEAM); develop laboratory, computer, and lecture  facilities; choose biomanufacturing courses or modules to  facilities; choose biomanufacturing courses or modules to fit local partner needs; and develop o.j.t. opportunities3. Provide opportunities for faculty, teacher and student  professional development.4. Partner with national organizations such as the National  Science Foundation Advanced Technological Centers, Bio‐ S i F d ti Ad dT h l i lC t Bi Link and NBC2, BIO, SIM and ASM.
  45. 45. Biomanufacturing Course  Development D l tChoose existing textbooks and laboratory manuals or make your own in a Course in a Box  l k i C i Bapproach to providing your biomanufacturing students all they need to develop industrial  t d t ll th dt d l i d t i lstrength skills, knowledge and attributes to succeed in this advance technology  d i thi d t h lmanufacturing career path.
  46. 46. Individual Curriculum ModulesOver 75 biopharmaceutical biomanufacturing SOPs based on industry practices.11 biopharmaceutical biomanufacturing chapters in textbook authored by industry.8 biofuels chapters in textbook authored by Elmar Schmid, representing both industry and education.4 biofuels labs based on industry practices.5 Protein is Cash modules Online support site at www.biomanonline.org. 
  47. 47. Course in a BoxThe NBC2 plan is to provide a ‘Course in a Box’ type of format to deliver the materials necessary  ff d li h i lto run one SOP or a collection of SOPs.  Two examples follow: l f ll Protein is Cash ‐ Day 3: Downstream Processing Biofuels Laboratory 2:  Using Cellulosic Enzymes  to Produce Bioethanol
  48. 48. Protein is Cash Course in a Box Protein is Cash ‐ Day 3: Downstream Processing Item Source Amount Cost SOP with Cover NBC2 20 each $ 5.00 GFP Chromatography Kit Bio‐Rad 1 each $89.00 Equipment • Mini‐Centrifuge Bio‐Rad 1 each • Microtube rack NBC2 10 each • Eppendorf Tubes (2ml) NBC2 1 bag (150) Supplies • E. coli ‐ GFP transformed 1 ml cryovial y • AEX Columns 10 each • CEX Columns 10 each • IEX Equilibration Buffer • IEX Elution Buffer 1 • IEX Elution Buffer 2 • GFP Standard GFP Standard 10 ml 10 ml • UV Pen Lights 10 each • Glass Hearts 20 each $33.00 Virtual Downstream Processing Module • CD • Thumb Drive • App • Subscription
  49. 49. Cellulosic Biofuels Course in a Box Biofuels Laboratory 2:  Using Cellulosic Enzymes to Produce Bioethanol Item Source Amount Cost SOP with Cover NBC2 20 each $ 5.00 Cellulosic Biofuels Kit Bio‐Rad 1 each $89.00 Equipment • Smart Spectrophotometer Bio‐Rad 1 each • Shaking Water Bath g Fisher 1 each
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