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11 © 2009 PerkinElmer© 2009 PerkinElmer© 2009 PerkinElmer© 2013 Digital World BiologyMolecular Murder MysteriesSandra Port...
22Thanks to…www.bio-link.orgBiotech-careers.orgNWABR.orgNational Science FoundationBio-ITEST, DRL 0833779National Science ...
33OverviewPracticeDivide into groups – up to 7Solve mysteriesPresent resultsSchedule
44 Not just for barcoding!Cytochrome C OxidaseA BC D
55Gene name Protein name Gene location 1OCC chainMT-COI cytochrome c oxidase subunit I Mitochondrion AMT-CO2 cytochrome c ...
66 Not just for barcoding!Cytochrome C OxidaseO2H2O
77Goal – to identify the substance bound to the active siteCome up with a mystery story to explain what happenedPrerequisi...
88Go to figshare.comSearch for cytochrome C oxidase – see lots of structures Download 1OCC.cn3 – cytochrome C oxidaseLook...
99Molecular Murder Mysteries
1010Positively charged amino acidMolecular Murder Mysteries
1111Molecular Murder MysteriesGroup Structure What is bound to the active site?Control 1OCC_3ABM_anno1.cn3 Oxygen – normal...
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Molecular murder mysteries 2013

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Transcript of "Molecular murder mysteries 2013"

  1. 1. 11 © 2009 PerkinElmer© 2009 PerkinElmer© 2009 PerkinElmer© 2013 Digital World BiologyMolecular Murder MysteriesSandra Porter, Ph.D. @digitalbio,sandra@digitalworldbiology.com
  2. 2. 22Thanks to…www.bio-link.orgBiotech-careers.orgNWABR.orgNational Science FoundationBio-ITEST, DRL 0833779National Science FoundationBio-Link DUE 0903317
  3. 3. 33OverviewPracticeDivide into groups – up to 7Solve mysteriesPresent resultsSchedule
  4. 4. 44 Not just for barcoding!Cytochrome C OxidaseA BC D
  5. 5. 55Gene name Protein name Gene location 1OCC chainMT-COI cytochrome c oxidase subunit I Mitochondrion AMT-CO2 cytochrome c oxidase subunit II Mitochondrion BMT-CO3 cytochrome c oxidase III Mitochondrion CCOX4I1 cytochrome c oxidase subunit IV isoform 1 Chromosome 16 DCOX4I2 cytochrome c oxidase subunit IV isoform 2 (lung) Chromosome 20 DCOX5A cytochrome c oxidase subunit Va Chromosome 15 ECOX5B cytochrome c oxidase subunit Vb Chromosome 2 FCOX6A1 cytochrome c oxidase subunit VIa polypeptide 1 Chromosome 12 GCOX6A2 cytochrome c oxidase subunit VIa polypeptide 2 Chromosome 16 GCOX6B1 cytochrome c oxidase subunit VIb polypeptide 1 Chromosome 19 HCOX6B2 cytochrome c oxidase subunit VIb polypeptide 2 (testis) Chromosome 19 HCOX6C cytochrome c oxidase subunit VIc Chromosome 8 ICOX7A1 cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) Chromosome 19 JCOX7A2 cytochrome c oxidase subunit VIIa polypeptide 2 (liver) Chromosome 6 JCOX7B cytochrome c oxidase subunit VIIb Chromosome X KCOX7B2 cytochrome c oxidase subunit VIIb2 Chromosome 4 KCOX7C cytochrome c oxidase subunit VIIc Chromosome 5 LCOX8A cytochrome c oxidase subunit VIIIA Chromosome 11 MCOX8C cytochrome c oxidase subunit VIIIC Chromosome 14 MNot just for barcoding!Cytochrome C Oxidase
  6. 6. 66 Not just for barcoding!Cytochrome C OxidaseO2H2O
  7. 7. 77Goal – to identify the substance bound to the active siteCome up with a mystery story to explain what happenedPrerequisite knowledge Identify 3 key elements (oxygen, nitrogen, carbon) Use Wikipedia Be familiar with the concept of an active site Use Cn3DMolecular Murder Mysteries
  8. 8. 88Go to figshare.comSearch for cytochrome C oxidase – see lots of structures Download 1OCC.cn3 – cytochrome C oxidaseLook at features of structurePractice with Cn3DFigure out colors for elementsMolecular Murder Mysteries
  9. 9. 99Molecular Murder Mysteries
  10. 10. 1010Positively charged amino acidMolecular Murder Mysteries
  11. 11. 1111Molecular Murder MysteriesGroup Structure What is bound to the active site?Control 1OCC_3ABM_anno1.cn3 Oxygen – normal function of protein reducesO2 to H2OA 1OCC_1OCOB 1OCC_1OCZC 1OCC_3AG1D 1OCC_3AG2E 1OCC_3AG3F 1OCC_3AG4G 1OCC_3ABK

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