Development of a Technical Program in Stem Cell Science

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Dr. Thomas Tubon from Madison College describes the process of developing a program in stem cell technology.

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Development of a Technical Program in Stem Cell Science

  1. 1. Development of a Technical Program in Stem Cell Science: Responding to an Emerging Need National Science Foundation Advanced Technological Education DUE 1104210
  2. 2. Madison College • 2012 Centennial Celebration •Over 140 career paths serving more than 45,000 students, including many of the fastest growing occupations identified by the WDWD. •12 Campuses (8 in Madison, 4 Regional). •Greater than 90% placement rate for graduates within 6 months. •Technical colleges generate nearly $7 billion in economic benefits for Wisconsin each year.Facilities Expansion Health Education Ingenuity Center Protective Services
  3. 3. Biotechnology in Wisconsin • Between 2004 and 2009 bioscience employment in Wisconsin grew by nearly 3% in contrast to the rest of the economy that shrunk by > 3% • The average bioscience worker has earnings that are 64% higher than the earnings of a typical Wisconsin employee • More than 640 Wisconsin bioscience businesses have created nearly 24,000 private sector jobs with a total economic impact of close to $7 billion
  4. 4. •Reported industry revenues in 2005 totaled $974,000 and $36.9 million in 2007. In the US, by 2016, stem cell company revenues are projected to exceed $8.5 billion and $16-20 billion by 2020. •The state of Wisconsin has invested more than $94 million, in addition to private investments to promote growth in the stem cell and regenerative biology sector. •California Institute of Regenerative Medicine (CIRM) $3 billion in Grants. The Bioeconomic impact of Stem Cell Science
  5. 5. Stem Cells & Regenerative Biology At Madison College • In 2007, Madison College piloted our first human stem cell course. •In 2011, NSF awarded $851,454 to develop a technical education program in Emerging Stem Cell Technologies. •40 Advisory Board Members: Stem Cell Industry, UW Madison SCRMC, UW Waisman Center for Developmental Disorders, and Morgridge Institute for Research / Wisconsin Institute for Discovery.
  6. 6. Development of a Technical Program in Stem Cell Technologies: Responding to an Emerging Need (NSF DUE 1104210) BJECTIVES: I. Development of a 2-semester certificate program emphasizing workforce training in Stem Cell Technologies II. Develop educational materials in stem cell technologies for dissemination at the local, regional, and national levels. (web-based distribution, instructional videos, manuals) III. Promote the growth of Stem Cell Programs in other Colleges & Universities throughout the nation
  7. 7. Introduction to human Stem Cell Methods: Basic Culture and Characterization Course Competencies: Characterization of hESCs • Chromosomal analysis, florescence microscopy • Media Formulation (Feeder-dependent/xeno-free media) •Generation of Cell Aggregates (EBs) hESC H9 DAPI Stain For metaphase chromosomes hESC H9 Cell Aggregates: Embryoid formation
  8. 8. Scheduled Meeting time: ecture: Monday 5:00pm to 5:50pm aboratory: Monday/Thursday 6:00pm-8:50pm arget audience: AAS Biotechnology, BS in Biological Sciences at or nearing completion Post-Baccalaureate, Graduate Students, Laboratory Staff. Human Stem Cell Technologies Certificate Program: 4 Courses (8 Credits)
  9. 9. SEMESTER 1: Introduction to human Stem Cells Basic Culture and Characterization Course Competencies: • Stem Cells and Bioethics • Aseptic techniques and routine maintenance of PSC cell cultures. • Basic techniques for culturing hES (H9) cells: thawing, plating, feeding, passaging, and cell banking Pluripotent Stem Cell colony pre-split (10x) Pluripotent Stem Cell colony pre-split (2.5x)
  10. 10. Course Competencies: • hES culture systems: Feeder dependent/independent. hESC H9 Colony on MEF-feeder layer (20x) hESC H9 Colony – Feeder independent On Matrigel (10x) SEMESTER 1: Introduction to human Stem Cells Basic Culture and Characterization
  11. 11. Course Competencies: • hESC (H9) and iPS (iMR90-4) Cell Differentiation Spontaneous & Directed Differentiation of Adherent Cells and Cell Aggregates •Immunoflourescence Microscopy & Molecular Analysis of PSC-derived differentiated cells SEMESTER 2: Advanced human Stem Cells- Differentiation & Applications Hepatocyte Differentiation Neural Rosette Differentiation Cardiomyocyte Differentiation
  12. 12. Advanced Cell Culture Education Suite (ACCES) •2744 sq.ft. Animal Cell Culture Facility. •Construction completed and fully operational, January 2012.
  13. 13. Advanced Cell Culture Education Suite (ACCES) Molecular Wet Lab Cell Culture Imaging & Microscopy
  14. 14. Neuronal Differentiation Neural Differentiation of Human Embryonic Stem Cells Anderson, B., Bagnall, J., Holston, N., Jefferson, K., Ledbury, B., Rosenbaum, H., Schmidt, C., Schreiber, S., Skinner, J., Steckenfinger, S., Stephens, D., and Sugden, D. Madison Area Technical College, Biotechnology Program Human Stem Cell Education Certificate 1701 Wright St. Madison, Wisconsin 53704 (608)246-6875 •Utilizing the Zhang Protocol, we were able to differentiate human embryonic stem cells towards ectodermal derivatives and further to neural cell-types. •Verification by cell morphology and immunocytochemistry demonstrates that the stem cells followed the expected pathway for neural differentiation. •The next step is to perform RT-qPCR for confirmation of specific gene expression, including Pax6 and β-tubulin. •The cells are still in the process of culturing, and will continue down the neural lineage pathway until they form mature neural networks. Support for the development of the StemCell Education Initiative isprovided through aNational Science FoundationAdvanced Technological Education Grant (DUE1104210)awarded to Madison College. Aspecial thanksto WiCell, CDI, &Life Technologiesfor providingcells and reagentsto support our program. Conclusions Reference Acknowledgments Introduction Emerging stem cell science provide the basis for today’s most promising technologies with far reaching implications in both research and medicine. Current and potential applications include: replacement of damaged or diseased tissue, studying human development, testing of new drugs, screening for potential toxins, developing new methods for gene therapy, and likely many other yet to be determined future uses. As the field of stem cells and regenerative medicine continues to evolve, our understanding of the science leads into methods of cell differentiation into even more specific cell types which were previously not possible. This paradigm shift will undoubtedly change the way medicine is performed from the conventional methods used today allowing us to precisely target and replace damaged, malfunctioning, and aging cells. DUE 1104210 Results H9 Cultured on MEFs Day 3: EBs Formation in NIM Day 10: Early Rosette formation Day 14 : Late Rosette Formation Day 24: Neurospheres in NDM Day 36: Immature Neurons Pluripotent StemCell Media(PSC): •DMEM/F12 •KOSR •MEM NEAA •bFGF L-Glutamine + BME Neural Induction Media (NIM): •DMEM/F12 Glutamax •NEAA •Heparin •N2 Supplement Neural Differentiation Media (NDM) •Neurobasal Media •Glutamax •30% Glucose •B27 •cAMP •Ascorbic Acid •N2 Supplement •GDNF, BDNF, and NT3 Media PreparationsMethods Directed differentiation was achieved by growth in selective media preparations NIM and NDM, according to standard formulation (Hu, B and Zhang, S.C, 2010) Pax6 DAPI BetaTubulin DAPI Hu, Bao-Yang and Su-Chun Zhang, 2010. Methods Mol Biol. Directed differentiation of neural-stem cells and subtlype-specific neurons from hESCs, 636, 123-127 Day 42 Phase contrast 2013 Biopharmaceutical Technical Center Institute STEM CELL SYMPOSIUM
  15. 15. Summary : Stem Cell Education With NSF ATE support, we have developed a technical training program in Human Stem Cells to address the emerging demands of the global bioeconomy. Programming also includes the creation of 1-day workshops & a 5-day Short Course
  16. 16. Stem Cells & Regenerative Biology In Madison, Wisconsin
  17. 17. Thank you! Supported by the National Science Foundation Advanced Technological Education Project Grant DUE 1104210 Awarded to Madison College July 2011
  18. 18. Contact Information •Lisa Seidman, Ph.D PI, Biotechnology Program Director (608) 246-6204 lseidman@matcmadison.edu •Thomas Tubon, Ph.D. Project Director/Co-PI, NSF Stem Cell Program at Madison Colleg (608)246-6875 tubon@matcmadison.edu •Jeanette Mowery, Ph.D. Co-PI, Faculty jmowery@matcmadison.edu

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