Bioreactor education & training w.marner ccp13
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Bioreactor education & training w.marner ccp13

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Technicians' Role in Growing the Bioeconomy

Technicians' Role in Growing the Bioeconomy

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  • 1. www.applikonbio.comSlide 1Technicians Role in Growing theBioeconomy PanelWesley D. Marner II, Ph.D.22 April 2013
  • 2. www.applikonbio.comSlide 2Benchtop(1-20L)Pilot/Production(7-2,000L)Applikon at a glance• Worldwide provider ofbioreactor equipment.
  • 3. www.applikonbio.comSlide 3Bioprocessing has traditionally been at larger culturevolumesManufacturingProcess Development and Scale UpUpstream Research• Bioreactor operation• Seed culture manipulation• Harvest and downstream purification• Equipment• Data analysis• Experimental design• Regulatory requirements• cGMP protocols• Industrial safety
  • 4. www.applikonbio.comSlide 4Consider early development screening...• Early stage "winners" can lock in the route ofdevelopment work during scale up to production10,000 1,000 150 25“Winners”≠Unfortunately,
  • 5. www.applikonbio.comSlide 5Trend toward greater bioprocessing examination,sooner.ManufacturingProcess Development and Scale UpUpstream ResearchNumberofCultures# of Cultures Cost / Culture Process InformationCostperCulture
  • 6. www.applikonbio.comSlide 6Trend toward greater bioprocessingexamination, sooner.ManufacturingProcess Development and Scale UpUpstream ResearchNumberofCultures# of Cultures Cost / Culture Process InformationCostperCultureAcquiring processdata sooner.
  • 7. www.applikonbio.comSlide 7Trend toward greater bioprocessingexamination, sooner.ManufacturingProcess Development and Scale UpUpstream ResearchNumberofCultures# of Cultures Cost / Culture Process InformationCostperCultureReducing cost of earlydevelopment.Acquiring processdata sooner.
  • 8. www.applikonbio.comSlide 8Micro(200µl)Benchtop(1-20L)Pilot/Production(7-2,000L)Mini(0.25-3L)Applikon at a glance• Worldwide provider ofbioreactor equipmentat all scales.
  • 9. www.applikonbio.comSlide 9New technology for bioprocessing at small scales:Sensor Shake FlasksOD ReadersSensor DishesRAMbioMini BioreactorsParallel Off-GasWell PlatesProcess Optimization:Bioreactor ExperimentsProcess Definition:Well Plates,Flask CharacterizationStrain Construction:Tubes, Well PlatesFlasks, ShakersIdentification:Cloning, Fluorescence, Well Plates, FlasksProcess DevelopmentScreeningSelectionResearchClassicAutoclavables
  • 10. www.applikonbio.comSlide 10• Bioprocessing principles in upstream research• Bioreactor startup and operation• Bioreactor control & typical parameters• Culture modes (batch, fed-batch, continuous)• Culture kinetics (growth, nutrient uptake, product formation)• Bioengineering basics (gas transfer, mixing)• Understanding the abilities and limitations of small-scale tools• Small bioreactors vs. large one• Artifacts of microtiter plates• Advantages/Disadvantages of scaling down• Data analysis and statistics - and communicating these data
  • 11. www.applikonbio.comSlide 11Thank you• www.applikonbio.com/wmarner• wmarner@applikonbio.com