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Stocking the Breeder’s Toolbox:An update on the status of Ug99 resistance
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Stocking the Breeder’s Toolbox: An update on the status of Ug99 resistance


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Mike Pumphrey, Washington State University

Mike Pumphrey, Washington State University

Published in: Technology, Health & Medicine
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  • 1. Stocking theBreeder’sToolbox:An update onthe status ofUg99 resistanceMike PumphreySept 2,
  • 2. DRRW Pre-breeding efforts:CIMMYTCornell Univ.CSIROEIARICCI-Tel Aviv Univ.Kansas State Univ.Univ. of AdelaideUC-DavisUniv. of the Free StateUniv. of MinnesotaUniv. of SydneyUSDA-ARSWashington State Univ.
  • 3. Why do we need a better toolbox? Speedand support replacement of rust susceptible varieties with varieties based on complex resistance - globally Increase diversity of resistance and promote durable resistance Enablestewardship of resistance sources in a manner never before possible
  • 4. What tools are needed?A well-surveyed resistance gene landscape Additional, useful, sources of resistance Effective gene pyramids DNA markers and supporting information for robust selection/diagnostics Good germplasm
  • 5. Deploying COMPLEX resistanceSeedling/All-stage 3 or more effective R genes combinedAdult plant/Slow-rusting 3 or more effective APR genes combined
  • 6. COMPLEX resistance % Rust Severity Susceptible 100 80 1 to 2 APR genes 60 or 1+ race-specific gene 40 2 to 3 APR genes or 1+ race-specific gene 20 4 to 5 APR genes 0 or 1+ race-specific gene 0 10 20 30 3+ 40 50 Day of Epidemic This is the goal!
  • 7. Ug99-family Resistance Loci(circa 2008) Red genes are translocations or show reduced recombination Green genes are APR loci Black genes have normal recombination Sr1A.1R Sr27 Sr21 Sr35 Sr26 Sr22 Sr131A 2A 3A 4A 5A 6A 7A Sr39 Sr2 Sr40 Sr37 Sr281B 2B 3B 4B 5B 6B 7B Sr33 Sr32 Sr45 Sr42 Sr44 Sr50 Sr25 Sr29 Sr431D 2D 3D 4D 5D 6D 7D Alien introgression with linkage drag
  • 8. Ug99-family Resistance Loci(2012) Red genes are translocations or show reduced recombination Green genes are APR loci Black genes have normal recombination SrTr129 Sr1A.1R Sr27 Sr21 Sr35 QTL Sr26 Sr22 SrTm4 Sr52 QTL SrND643 Sr13 Sr151A 2A 3A 4A 5A 6A 7A Sr39 Sr2 Sr40 Sr12 QTL QTL QTL SrWeb/ Sr37 Gabo 56 QTL QTL Sr28 Sr56 Lr46 Sr471B 2B 3B 4B 5B 6B 7B Sr1662 Sr33 Sr32 Sr57 Sr45 Sr51 Sr42 Sr44 Sr46 Sr50 SrAt6D SrAt7D Sr53 Sr25 SrSha7 Sr55 SrC Sr29 Sr431D 2D 3D 4D 5D 6D 7D
  • 9. Sources of Ug99 resistanceGene Chrom. Origin NotesSr2 3BS Tetraploid wheat APRSr12 3BS Tetraploid wheat Moderate/background eff.Sr13 6AL Tetraploid wheatSr15 7AL Hexaploid wheat Moderate/background eff.Sr22 7AL Triticum monococcum Diagnostic markersSr25 7DL Thinopyrum ponticum Diagnostic markersSr26 6AL Thinopyrum ponticum Diagnostic markersSr28 2BL Hexaploid wheat Linkage block, diagnosticSr32 2DS Aegilops speltoides New recombinantsSr33 1DS Aegilops tauschii Linkage block, clonedSr35 3AL Triticum monococcum ClonedSr37 4B Triticum timopheevii New recombinants
  • 10. Sources of Ug99 resistanceGene Chrom. Origin NotesSr39 2BS Aegilops speltoides New recombinantsSr40 2BS Triticum timopheevii New recombinantsSr42 6DS Hexaploid wheat SrCad?, SrNini?Sr43 7DL Thinopyrum elongatum New recombinantsSr44 7DS Th. intermedium New translocationSr45 1DS Aegilops tauschii Linkage blockSr46 2DS Aegilops tauschiiSr47 2BL Aegilops speltoides New recombinantsSr50 1DS Rye Linkage blockSr51 3DS Aegilops searsii Recombinants underwaySr52 6AL Dasypyrum villosum Temperature sensitiveSr53 5DL Aegilops geniculata New recombinants
  • 11. Sources of Ug99 resistanceGene Chrom. Origin NotesSr55 4DL Hexaploid wheat APR-Lr67/Yr46Sr56 5BL Hexaploid wheat APRSr57 7DS Hexaploid wheat APR-Lr34/Yr18, clonedLr46 1BL Hexaploid wheat APR-Yr29Temporary DesignationsSrWeb/ 2BL Hexaploid wheat Linkage blockGabo56Sr1662 1DS Aegilops tauschiiSrTr129 6ASSrTm4 2A Triticum monococcumSrAt6D 6D Aegilops tauschiiSrAt7D 7D Aegilops tauschii
  • 12. Sources of Ug99 resistanceGene Chrom. Origin NotesTemporary DesignationsSrSha7SrCSrNini 6DSSrND643 4ALConsistent QTL (by association mapping or bi-parental mapping)1A, 1B, CIMMYT germplasm via2B, 3D, bi-parental and AM4A, 5B, approaches6B, 7A,7B
  • 13. Several additional sources andbetter tools are coming (frommany directions):A few DRRW project examples
  • 14. Discovery from wheat relatives  Introgression, allelism tests, mapping , chromosome engineering  Aegilops species, wild tetraploids, Da sypyrum villosum  Association mapping
  • 15. Cytogenetic improvement  Thinopyrum intermedium- Group 2
  • 16. Marker developmentSequencing, 9000 SNP, 90,000K SNPplatforms accelerating progress  Consolidated/standardized/dense90,000 SNP genotyping  ~160 Sr resistance sources from USDA-ARS  ~90 Lr resistance sources from USDA-ARS  ~400 Yr resistance sources from USDA-ARS Washington State  Numerous populations by 9K or 90KMap-based cloning (Sr33, Sr35, others)
  • 17. Linkage Block DevelopmentRecombininglinked loci toenhancestewardshipand breedingease
  • 18. Genomic selection/prediction
  • 19. So what? Therehas never been a more opportune time for the international community to embrace strategic deployment and responsible stewardship of our genetic resources for durable stem rust control … ……before we lose tools that we can’t afford to replace.
  • 20. Acknowledgements BMGF/DFID Cornell University Collaborator institutions BGRI IPP-CAAS