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Novel resistance to rust pathogens in bread wheat                  Li Huang            Montana State University
Mutagenesis  Alpowa--Susceptible line            Leaf rust screening  Treat seeds with EMS             Select for resista...
Al-180     Al-220        Al-268         Al-271         Al-535      Alpowa   MNs        MNs         MNs              MNs   ...
Al-220‘Alpowa’   MNs
Infection types of the mutant and the wild type                with Pt race PBJL             3 dpi                   6 dpi...
Infection types of the mutant and the wild type       with Pt race PBJL at seedling stage                                 ...
Infection types with Pt race PBJL            at adult stage                                 Al-220           ‘Alpowa’     ...
Infection types with Pt race PBJL                  at seedling stage    Susceptible                   Resistance8   11    ...
Infection types with Pgt race TMLK          at seedling stage                                 Al-220           ‘Alpowa’   ...
Al-220Infection types of MNs and ‘Alpowa’ with races             of three rust pathogens                                  ...
Infection types with a local Egt race          at seedling stage                                Al-220           ‘Alpowa’ ...
Seedling stagePR Genes        Enzyme Actvity  PR1  PR2      β-1,3-Glucanase   PR3     Endochitinase   PR4     Endochitinas...
Al-220MNs‘Alpowa’
Al-220 Genetic analysis of resistance in the MNs                                          F2 individuals                  ...
2BS                                cM                           CentromereGenetics map of Lr48                            ...
AcknowledgementThe Huang Lab      Jackie Campbell      Dr. Hongtao ZhangCollaborators       Dr. Mike Giroux     MSU       ...
Novel Resistance to Rust Pathogens in Bread Wheat
Novel Resistance to Rust Pathogens in Bread Wheat
Novel Resistance to Rust Pathogens in Bread Wheat
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Novel Resistance to Rust Pathogens in Bread Wheat

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Li Huang, Montana State University

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Transcript of "Novel Resistance to Rust Pathogens in Bread Wheat"

  1. 1. Novel resistance to rust pathogens in bread wheat Li Huang Montana State University
  2. 2. Mutagenesis Alpowa--Susceptible line Leaf rust screening Treat seeds with EMS Select for resistance ones Mutation density: 1/12 kb (Feiz et al., 2009, Genetics)
  3. 3. Al-180 Al-220 Al-268 Al-271 Al-535 Alpowa MNs MNs MNs MNs MNs APRPt S R R R R R S R R R S SPgt R R R R R SPst APR S R R S S S
  4. 4. Al-220‘Alpowa’ MNs
  5. 5. Infection types of the mutant and the wild type with Pt race PBJL 3 dpi 6 dpi Al-220 Al-220 ‘Alpowa’ MNs ‘Alpowa’ MNs
  6. 6. Infection types of the mutant and the wild type with Pt race PBJL at seedling stage Al-220 ‘Alpowa’ MNs dpi 12 15 18 21 12 15 18 21
  7. 7. Infection types with Pt race PBJL at adult stage Al-220 ‘Alpowa’ MNsdpi 18 21 24 18 21 24
  8. 8. Infection types with Pt race PBJL at seedling stage Susceptible Resistance8 11 14 17 20 8 11 14 17 20 Chinese Spring x MNsAl-220
  9. 9. Infection types with Pgt race TMLK at seedling stage Al-220 ‘Alpowa’ MNsdpi 18 21 24 18 21 24
  10. 10. Al-220Infection types of MNs and ‘Alpowa’ with races of three rust pathogens Lines Pathogens Races Stage Al-220 ‘Alpowa’ MNs PBJL Seedling 3~4 ;~2 Adult S R Pt SBGD Adult S I BBBDD Adult S I 78 Seedling 8 7 127 Seedling 8 8 Pst Adult R R 101/102 Adult S R TLMK Seedling 3-~4 ;1+ QFCS 3~4 ;1+ TTKSK 3+ ;1+ TTKST 3+ ;1+ Pgt TTTSK 3+ ; TPMK 4 ; TTTT 4 ;1+ QTHJ 3+ ;1+
  11. 11. Infection types with a local Egt race at seedling stage Al-220 ‘Alpowa’ MNsdpi 18 21 24 18 21 24
  12. 12. Seedling stagePR Genes Enzyme Actvity PR1 PR2 β-1,3-Glucanase PR3 Endochitinase PR4 Endochitinase PR5 Thaumatin-like PR9 Lignin-forming Peroxidase PR10 RNase Adult stage
  13. 13. Al-220MNs‘Alpowa’
  14. 14. Al-220 Genetic analysis of resistance in the MNs F2 individuals Expected Crosses Ratio X2 Resistant Susceptible Al-220 MNs / ‘Alpowa’ 210 71 3:1 0.67 Al-220‘Chinese Spring’ / MNs 100 36 3:1 0.69
  15. 15. 2BS cM CentromereGenetics map of Lr48 Chinese Spring 2B Bin MapBansal et al., 2008, TAG Genetic map of MNsAL220 GrainGenes
  16. 16. AcknowledgementThe Huang Lab Jackie Campbell Dr. Hongtao ZhangCollaborators Dr. Mike Giroux MSU Dr. Yue Jin USDA-ARS, Cereal Disease Lab Dr. Meinan Wang WSU Dr. Xianming Chen WSU Dr. Deven See WSUFunding

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