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Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
Global Rust Reference Center: Current Research and Services
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Global Rust Reference Center: Current Research and Services

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Mogens S Hovmøller, Annemarie F Justesen, Stephanie Walter, Chris K Sørensen, Tine Thach, Julian R Algaba, Poul Lassen, Jens G Hansen

Mogens S Hovmøller, Annemarie F Justesen, Stephanie Walter, Chris K Sørensen, Tine Thach, Julian R Algaba, Poul Lassen, Jens G Hansen

Published in: Education, Technology
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  • 1. Global Rust Reference Center (GRRC): Current research and servicesCurrent research and services Mogens S Hovmøller, Annemarie F Justesen, Stephanie Walter, Chris K Sørensen, Tine Thach, Julian R Algaba, Poul Lassen, Jens G Hansen
  • 2. The Borlaug Global Rust Initiative NetworkThe Borlaug Global Rust Initiative Network GRRC, Aarhus University Denmark
  • 3. GRRC established in 2008 with support from CIMMYT, ICARDA and Aarhus University • Quarantine facility which can recieve live samples of wheat rust year roundp y (pathotyping) • Store alive isolates of incoming samples &• Store alive isolates of incoming samples & take responsibility for ”historic” Stubbs collection (Wageningen University NL)collection (Wageningen University, NL) • Assist in training of scientists in wheat rust pathology
  • 4. www.wheatrust.org
  • 5. Type of GRRC activities, funding bodies and budget Applied Research national International Activities National Strategic Research pHd Education Annual average 2013-2015: ~ 1.6 M US$
  • 6. WheatWheat rustrust quarantinequarantine facilitfacilitfacilityfacility
  • 7. N2N2 storagestorage of liveof live wheatwheat rustrust isolatesisolates ~ 8000 samples from recent years
  • 8. N2N2 storagestorage of liveof live wheatwheat rustrust isolatesisolates ~ 5000 samples from historic ”Stubbs” collection
  • 9. New GRRC standard procedure for P. striiformis sample handling prior to virulence phenotyping and DNA genotyping Plant inoculation Excision of single lesion from leaf sample and induction of sporulation on Isolate multiplication and subsequent pathotyping p wet Whatman paper Excision of single lesion from dry leafSSR genotyping from dry leafSSR genotyping with ABI3730XL DNA Analyzer DNA extraction Q Multiplex PCR from 75ng DNA with 20 SSR primers (Ali et al BMC Research with Qiagen DNeasy Plant Mini Kit (Ali et al. BMC Research Notes 2011, 4:240) in 2 Multiplex reactions
  • 10. GRRC Wheat Rust Sample Summary 2013y
  • 11. GRRC Wheat Rust Sample Summary 2013y
  • 12. T h t t iTwo short stories 1. Origin and spread of two aggressive1. Origin and spread of two aggressive strains of P. striiformis 2 U d di h i l h2. Understanding the virulence phenotype (case: Yr17)( )
  • 13. P. Striiformis aggressiveness Increased aggressiveness: Refers to a pathogen isolate that causes disease more quick and severe on aq susceptible host (compatible interaction) - compared to a reference isolatereference isolate • Susceptible cv. Cartago • 12 days after inoculation i b t d i th Non-aggressive Aggressive strain - incubated in the same environment strain NA 1990 NA 2005
  • 14. P. Striiformis aggressiveness Walter et al. 2012: 570 isolates from 40 countries• 570 isolates from 40 countries and six continents (recent collections by GRRC and references from the historicreferences from the historic “Stubbs” collection) AAssays: • 3 SCAR markers (developed from AFLP polymorphic sites) d 20 SSR kand 20 SSR markers were applied to assess genetic association to PstS1 and PstS2 ( i t i ) Non-aggressive Aggressive strain (aggressive strains) • 15 virulences based on 25-30 wheat differential lines strain NA 1990 NA 2005
  • 15. 2. Understanding the virulence phenotype Example: phenotyping virulence to Yr17 derived from T. ventricosum/ VPM1 Yr17 virulence Yr17 avirulence Yr17 avirulence? Yr17 virulence?Yr17 virulence Yr17 avirulence Yr17 avirulence?
  • 16. 2. Understanding the virulence phenotype New exotic race appeared in high frequency in Europe in 2011: Ambition (DK)/Warrior (UK) Conclusions (preliminary)Conclusions (preliminary) • Isolates of Ambition/Warrior race heterozygous for virulence to Yr17 • Virulence to Yr17 dominant or co- dominant
  • 17. Concluding remarks • Global Rust Reference Center (GRRC) is running and expanding – emphasis on yellow (stripe) and stem rust • Isolate collections expanding, - contributing to the understanding of host-pathogen interactions at the genetic and epidemiological level • Wheat Rust Toolbox provide a new tool for rapidp p dissemination and interpretation of a wide array of results related to ’wheat rust epidemiology’ • Significant increase in activities in recent years (project funding and investment in facilities by Aarhus University)
  • 18. Acknowledgements International collaborators • Kumarse Nazari, ICARDA • Amor Yahyaoui, ICARDA R i Si h CIMMYT GRRC-team • Ravi Singh, CIMMYT • Dave Hudson, CIMMYT • Claude Pope, INRA (F) • Jonathan Yuen, SLU (S) • Cristobal Uauy, JIC (UK) • Rosemary Bayles, NIAB (UK)ose a y ay es, (U ) • Kerstin Flath, JKI (D) • James Brown, JIC(UK) Sajid Ali (P)• Sajid Ali, (P) • > 40 people who submitted samples from Asia, Africa and South America

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