Bios 7901 - 12
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Analysis, design, implementation and testing of an optoelectronic system with a high power infared laser diode for cancer therapy using gold nanoparticles. Animal (murine) model.

Analysis, design, implementation and testing of an optoelectronic system with a high power infared laser diode for cancer therapy using gold nanoparticles. Animal (murine) model.

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Bios 7901 - 12 Bios 7901 - 12 Presentation Transcript

  • Energy-based Treatment of Tissue and Assessment VIModelling and characterization of photothermaleffects assisted with gold nanorods in ex-vivo samples and in a murine model AUTHORS: Félix Rodríguez Jara1, Horacio Lamela Rivera2 and Vincent Cunningham3 1felix.rjara@alumnos.uc3m.es 2horacio@ing.uc3m.es 3vcunning.uc3m.es OPTOELECTRONICS AND LASER TECHNOLOGY GROUP ELECTRONIC TECHNOLOGY DEPARTMENT San Francisco (CA), January 23th, 2011
  • 0. General Index.1. Introduction General Index: 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design. 1. Introduction2. Opto-thermal modelling for photo-thermal 2. Opto-thermal modelling for Photo-Thermal Therapy therapy. 2.1 Approach 2.2 Finite Element Modelling. 3. Experimental Results3. Experimental Results. 3.1 Experimental Set- up. 3.2 Experimental 4. Conclusions and Future Work Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work Modelling and characterization of photothermal effects assisted with 1 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction General Index: 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental Results. 1. Introduction 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work Modelling and characterization of photothermal effects assisted with 2 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 1.1 The Motivation. 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. CANCER, one of the main causes of mortality all around the world. 1.4 Nanotechnology in Photo-thermal therapy. - 7.4 Millions out of the total deaths per year ≈ 13 % (WHO) 1.5 The Design.2. Opto-thermal modelling for Need of investigation in new therapy techniques photo-thermal therapy. Mortality and side effects 2.1 Approach 2.2 Finite Element Modelling. Number of patients that can be trated3. Experimental Results. Laser hyperthermia technique 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo Collaboration with a specialized Company in4. Conclusions and Animal models Future Work - Good Laboratory Practices (GLP) - Qualified Staff - Ethical Committee Modelling and characterization of photothermal effects assisted with 3 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 1.2 The hyperthermia technique: the GOAL 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal Hyperthermia temperature Tumoral Cell death therapy. (from 42ºC to 65 ºC) 1.5 The Design. held during various minutes2. Opto-thermal GOAL: 80% Tumour (3-10’) modelling for photo-thermal tissue ablation therapy. 2.1 Approach 2.2 Finite Element Modelling. 42 ºC – 65 ºC3. Experimental Results. Tumour 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental 37 ºC Results – in-vivo 5-10 mm4. Conclusions and Future Work Biological Tissue Hyperthermia temperature selected: 55 ºC ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA 4 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO
  • 0. General Index. 1.2 The Hyperthermia Technique: State-of-1. Introduction 1.1 The Motivation The-Art 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. Registro Tª 1.5 The Design. Láseres alta potencia estado sólido (Nd:YAG, OPO)2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach Radiofrecuencia 2.2 Finite Element Modelling. Ultrasonidos (HIFU)3. Experimental Fibra óptica + Difusor Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Tumor Results – in-vivo4. Conclusions and Tejido biológico Future Work - Low selectivity - Use high amounts of energy - Very expensive devices with big dimensions Modelling and characterization of photothermal effects assisted with 5 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 1.3 The Photo-thermal Therapy System 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology HIGH in Photo-thermal POWER therapy. LASER 1.5 The Design. DIODE2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Temperature Modelling. Register3. Experimental Superficial Results. λ= 808 3.1 Experimental Set- nm up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo Nanoparticle Interna infusion:4. Conclusions and Intratumoural or Future Work Intravenous ? Biological Tissue Modelling and characterization of photothermal effects assisted with 6 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 1.4 Nanotechnology in Photo-Thermal Therapy 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology Gold Nanoparticles GOLD NANORODS in Photo-thermal therapy. 1.5 The Design. Tuned optical EFFICIENCY POWER2. Opto-thermal absorbance modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo Fig. adapted of “Cancer Research”, 69(9):1-9, (2009) 3.3 Experimental Results – in-vivo 1.5 - Passive A A Longitudinal4. Conclusions and Surface Plasmon Future Work - Harmless 1 Absorción [cm -1] Resonance Peak B B Axial 0.5 Surface Plasmon Resonance Peak 0 400 500 600 700 800 900 1000 1100 Longitud de onda [nm] Modelling and characterization of photothermal effects assisted with 7 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 1.5 The Design: Experimental studies 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for photo-thermal therapy. Efficiency Study Efficacy Study 2.1 Approach 2.2 Finite Element Modelling. CT-26 COLON CANCER OPTIMIZE THE SET-UP3. Experimental XENOGRAFT STUDIES Results. 3.1 Experimental Set- - Number of animales for group -> Statistics - Tumour size? up. 3.2 Experimental Results – ex-vivo - System design in terms of efficiency: - Stops Tumor growing? 3.3 Experimental Results – in-vivo (Power, Irradiance, Nanoparticle Concentration) - Ablation of the tumoral tissue?4. Conclusions and - Therapy parameters Future Work (Exposure time, way of application) - Ethical Committee (international directives of animal handling) -> HEATING UP, NOT BURNING OR CHARRRING ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA 8 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO
  • 0. General Index.1. Introduction General Index: 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental 2. Opto-thermal Modelling for Results. 3.1 Experimental Set- up. 3.2 Experimental Photo-Thermal Therapy Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA 10 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO
  • 0. General Index.1. Introduction 2.1 Approach 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. Energy Optical power, Temperature 1.5 The Design. Deposition Transferencia Irradiance Fuentes térmicas térmica2. Opto-thermal P [W], I [W/cm2] modelling for photo-thermal therapy. Optical properties Physical and thermal properties 2.1 Approach of the tissue of the tissue 2.2 Finite Element Modelling. µa [cm-1], µs [cm-1] ρ[kg/m ]C [JKg-1K-1, k [Wm-1K-1], 33. Experimental Results. Thermal Source 3.1 Experimental Set- up. 3.2 Experimental Optical Power Results – ex-vivo 3.3 Experimental Thermal Energy Results – in-vivo Transferred Temp4. Conclusions and Future Work dV dV Tissue absorption + Nanoparticles µtotal = µtissue+µnanoparticles Modelling and characterization of photothermal effects assisted with 11 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 2.2 Finite Element Modelling (FEM) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. - Less computational resources needed 1.4 Nanotechnology in Photo-thermal - It can be applied to complex geometries therapy. 1.5 The Design.2. Opto-thermal modelling for Energetic Initial Thermal photo-thermal Contribution. Temperature Energy therapy. Laser energy 37 ºC transference 2.1 Approach absorption 2.2 Finite Element Modelling.3. Experimental NO Results. 3.1 Experimental Set- Stop time Temperatures up. 3.2 Experimental reached? update Results – ex-vivo 3.3 Experimental Results – in-vivo YES4. Conclusions and Future Work Final Temperature Modelling and characterization of photothermal effects assisted with 14 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction General Index: 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental 3. Experimental Results Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work 15
  • 0. General Index.1. Introduction 3.1 Experimental Set-up (I) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. Optoelectronic General Schema 1.4 Nanotechnology Thermocouple in Photo-thermal therapy. thermometer 1.5 The Design. Sonda termopar2. Opto-thermal Lentes de hipodérmica modelling for photo-thermal Cabezal láser acoplo (HYP-1, Omega) Soporte therapy. (CNI-MDL-H-808-5000, CNI) d1 2.1 Approach 2.2 Finite Element Diámetro del haz Modelling. (FWHM) d03. Experimental Fibra óptica d2 Results. l1 l2 3.1 Experimental Set- (CNI-SMA-Fibre-600, CNI) up. 3.2 Experimental Results – ex-vivo l1 = l2 Tejido irradiado 3.3 Experimental f1= f2 = 2.54 cm Results – in-vivo D1 = D2= 2.54 cm Termómetro infrarrojo4. Conclusions and (OS-530LE, Omega) Future Work Driver (PSU-H-LED, CNI) Modelling and characterization of photothermal effects assisted with 16 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.1 Experimental Set-up (II) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. Experimental Set-up for therapy application in ex-vivo tissue samples 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work Modelling and characterization of photothermal effects assisted with 17 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.1 Experimental Set-up (and III) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. Experimental set-up for therapy application in mice (in-vivo) 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work Modelling and characterization of photothermal effects assisted with 18 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index. 3.1 Experimental Set-up:1. Introduction 1.1 The Motivation Development Stages 1.2 The hyperthermia technique. 1.3 The Photo-thermal Develpment Stages of the Photo-Thermal Therapy System therapy system. 1.4 Nanotechnology in Photo-thermal therapy. Requirements 1.5 The Design. and Goals Optimization loop Optimization of the2. Opto-thermal System modelling for photo-thermal therapy. 2.1 Approach Concept, Design Biological Model 2.2 Finite Element Experimental and Implementation ex-vivo Modelling. of the system (tissue samples) Results3. Experimental Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo Experimental Biological Model 3.3 Experimental in-vivo Results – in-vivo Results (ratones)4. Conclusions and Future Work Optimization Clinical of the Application System (Human Beings) Modelling and characterization of photothermal effects assisted with 19 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.2 Experimental results - ex-vivo (I) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. Sample preparation: 1.4 Nanotechnology in Photo-thermal - Fresh chicken muscle tissue. therapy. 1.5 The Design. - Previous marking for infrared thermometer alineation.2. Opto-thermal modelling for - Hypodermical infusion of nanoparticles (Ntracker 30-PM-850, NANOPARTz, photo-thermal saline solution PH = 7.4, 0.1 ml, OD = 25). therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work Modelling and characterization of photothermal effects assisted with 20 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.2 Experimental results - ex-vivo (II) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. Looking for the optimal irradiance, experimental results. 1.4 Nanotechnology in Photo-thermal therapy. 55 Pbeam = 1.25 W 1.5 The Design. Phaz = 0.5 W, IFWHM = 0.95 [W/cm2], Láser+NRds Phaz = 1.25 W, IFWHM = 2.38 [W/cm2], Láser+NRds2. Opto-thermal 50 Phaz = 0.75 W, IFWHM = 1.43 [W/cm2], Láser+NRds IFWHM = 2.38 W/cm2 Phaz = 0.5 W, FWHM = 0.95 [W/cm2 Phaz = 1.00 W, IFWHM =I 1.90 [W/cm2], Láser+NRds ], Solo Láser modelling for 40 Phaz = 1.25 W, IFWHM = 2.38 [W/cm2], Láser+NRds 2 ΔT = 31 ºC 45 Phaz = 0.75 W, IFWHM = 1.43 [W/cm ], Solo Láser photo-thermal Phaz = 0.5 W, IFWHM = 0.95 [W/cm2], Solo Láser  T [ºC] therapy. 40 Phaz = 1.00 W, IFWHM = ], Solo Láser 2 Phaz = 0.75 W, IFWHM = 1.43 [W/cm21.90 [W/cm ], Solo Láser 35 2.1 Approach Phaz = 1.00 W, IFWHM = 1.90 [W/cm22.38 [W/cm2], Solo Láser Phaz = 1.25 W, IFWHM = ], Solo Láser 2.2 Finite Element 35 Phaz = 1.25 W, IFWHM = 2.38 [W/cm2], Solo Láser Modelling. 30 303. Experimental Results. 25 25 3.1 Experimental Set-  T [ºC] up. 20 3.2 Experimental 20 50 100 150 200 250 300 Results – ex-vivo Tiempo de exposición [sg] 3.3 Experimental Results – in-vivo 154. Conclusions and Future Work 10 ΔT = 3 ºC 5 0 0 50 100 150 200 250 300 Tiempo de exposición [sg] Exposure Time [s] Modelling and characterization of photothermal effects assisted with 22 gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.3 Experimental Results - in-vivo (I) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. Animal model 1.4 Nanotechnology in Photo-thermal - Female mice, albines, BALB/c (BALB/cAnNHsd) , specific patogens free. therapy. 1.5 The Design. - Animals were handled by qualified staff in a company with the Good Laboratory Practice Certificate (GLP)2. Opto-thermal modelling for Animal preparation photo-thermal therapy. - Random distribution by weigth. 2.1 Approach - Identification of each animal. 2.2 Finite Element Modelling. Identification - Hair removing from the exposed area. marks Shaving of3. Experimental - Light anaesthesya Ketamine/Xilacine (10μl/g). irradiated area Results. 3.1 Experimental Set- - Hypodermic infusion of nanoparticles up. 3.2 Experimental (Gold Nanorods, Ntracker). Results – ex-vivo 3.3 Experimental - Laser irradiation exposure. Results – in-vivo4. Conclusions and Positioning Future Work platform Laser beam direction 27 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.3 Experimental Results - in-vivo (II) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal Study of the thermal increment induced as a function of laser irradiance. therapy system. 1.4 Nanotechnology in Photo-thermal Pbeam = 1.25 W therapy. 1.5 The Design. 35 IFWHM = 2.38 W/cm2 HYP: P = 0.5 W2. Opto-thermal modelling for SUP: P = 0.5 W HYP: P = 1 W ΔT = 29.9 ºC 30 photo-thermal SUP: P = 1 W therapy. HYP: P = 1.25 W 2.1 Approach SUP: P = 1.25 W 25 2.2 Finite Element Modelling.3. Experimental 20  T [ºC] Results. 3.1 Experimental Set- up. 3.2 Experimental 15 12 ºC Results – ex-vivo 3.3 Experimental Results – in-vivo 104. Conclusions and Future Work 5 0 0 50 100 150 200 240 Tiempo de exposición [sg] Exposure Time [s] 28 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.3 Experimental Results - in-vivo (III) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal Proof of concept, Phaz = 1.25 W therapy system. experimental results. 1.4 Nanotechnology in Photo-thermal IFWHM = 2.38 W/cm2 therapy. 1.5 The Design. 60 T = 57.8 ºC2. Opto-thermal modelling for 55 photo-thermal therapy. 2.1 Approach 2.2 Finite Element 50 Modelling. Temperatura [ºC] Temperature [ºC]3. Experimental 45 Results. 3.1 Experimental Set- T = 38.1 ºC up. 40 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo 354. Conclusions and HYP: Gold Nanorods + Láser Future Work SUP: Gold Nanorods + Láser 30 HYP: PBS + Láser SUP: PBS + Láser 25 0 100 200 300 400 500 600 Tiempo de exposición [sg] Exposure time [sg] 33 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.3 Experimental Results - in-vivo (IV) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal Aspect of the skin after irradiation. therapy system. 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for Skin exposed to the laser photo-thermal Superficiebeam expuesta de piel therapy. a la radiación láser 2.1 Approach 2.2 Finite Element Modelling.3. Experimental Results. 3.1 Experimental Set- up. 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work 34 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 3.3 Experimental Results - in-vivo (V) 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal Proof of concept, FEM therapy system. 1.4 Nanotechnology Modelling. in Photo-thermal therapy. 1.5 The Design. 162. Opto-thermal 14 modelling for photo-thermal 12 therapy. 2.1 Approach 2.2 Finite Element 10 Modelling.  T [ºC]3. Experimental 8 The superficial thermal gradient registered Results. 3.1 Experimental Set- experimentally was of 14.75 ºC, while the up. 3.2 Experimental 6 modelled one was of 14.86. This is an absolute Results – ex-vivo 3.3 Experimental 4 error less than 0.11 ºC (0.75 %) Results – in-vivo Superficial. Laser + NRds. EXP4. Conclusions and 2 Superficial. Laser + NRds. FEM Future Work 0 0 100 200 300 400 500 600 Exposure exposición [sg] Tiempo de time [sg] 35 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction General Index: 1.1 The Motivation 1.2 The hyperthermia technique. 1.3 The Photo-thermal therapy system. 1.4 Nanotechnology in Photo-thermal therapy. 1.5 The Design.2. Opto-thermal modelling for photo-thermal therapy. 2.1 Approach 2.2 Finite Element Modelling.3. Experimental 4. Conclusions and future Results. 3.1 Experimental Set- up. 3.2 Experimental work Results – ex-vivo 3.3 Experimental Results – in-vivo4. Conclusions and Future Work 37 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 4.1 Conclusions: 1.1 Motivation 1.2 The hyperthermia technique 1.3 Photo-thermal The laser hyperthermia therapy system using gold nanoparticles therapy system. 1.4 Nanotechnology has been demonstrated to be VIABLE in a mice model. in Photo-thermal therapy. 1.5 Design. Only the tissue injected with nanoparticles reaches hyperthermia2. Opto-thermal temperatures. modelling for photo-thermal therapy. 2.1 Approach The tissue exposed to the laser beam, but NO injected with 2.2 Energy Balance nanoparticles, remains unaltered (it does not reach hyperthermia 2.3 Implemention of the solution. FEM. 2.4 Finite Element temperature) Modelling.3. Experimental The stablished irradiance does not induce tissue charring or skin Results. 3.1 Experimental Set- burning. up. 3.2 Experimental Results – ex-vivo 3.3 Experimental The computational model implemented allows to make accurate Results – in-vivo estimations of the final temperature of the irradiated tissue with4. Conclusions and nanoparticles. Future Work 38 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • 0. General Index.1. Introduction 4.2 Future work 1.1 Motivation 1.2 The hyperthermia technique 1.3 Photo-thermal Modelling and implementation therapy system. 1.4 Nanotechnology in Photo-thermal therapy. Study and design of new techniques of optical energy irradiation: 1.5 Design. contact applicators, more than one laser source, pulsed light.2. Opto-thermal modelling for Design and test of an integrated control and monitorization photo-thermal therapy. system: 2.1 Approach Tissue Temp. 2.2 Energy Balance temperature register 2.3 Implemention of the solution. FEM. 2.4 Finite Element Modelling. Laser power3. Experimental Results. 3.1 Experimental Set- up. Experimental study 3.2 Experimental Results – ex-vivo 3.3 Experimental Results – in-vivo Tumor model in mice to test the efficacy of the system4. Conclusions and -Determine if the tumour growing is stopped and finally, the tumour is Future Work ablated. - 15 animals (BALB/c) - Cell line CT-26 mice colon cancer (CT26.WT) 39 Modelling and characterization of photothermal effects assisted with gold nanorods in ex-vivo samples and in a murine model
  • Thank you for your attention! Have a nice day. ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA40 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO