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Luca Vandi presentation

  1. 1. PROBIOTICS IN POULTRY NUTRITION __________________________ Cost or opportunity? Baromfi Congress – Budapest 1° Oct 2013 Luca Vandi Poultry Tech Manager EMA Biomin GmbH
  2. 2. Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013 MANY TIMES PROBIOTICS FAIL TO GIVE A RELIABLE RESPONSE IN THE BIRDS – WHY? USE OF SINGLE-STRAIN PROBIOTICS COLONIZATION OF JUST A PART OF THE GIT USE OF NOT SPECIE-SPECIFIC STRAINS • SLOWER REPLICATION • QUICK ELIMINATION (ONCE REGULAR CONSUMPTION OF THE PROBIOTIC HAS CEASED) – Tannock, 2001 WRONG POSITIONING GROWTH PROMOTION??
  3. 3. SO… IN ORDER TO OBTAIN A RELIABLE REPLY FROM THE HOST A PROBIOTIC SHOULD BE MULTI- STRAIN • COLONIZATION OF THE WHOLE GIT TRACT SPECIE- SPECIFIC (selected by the gut of the bird) • FASTER MULTIPLICATION • LOW ELIMINATION WELL POSITIONED • IMMUNOMODULATION • COMPETITIVE EXCLUSION LESS PATHOGEN PASSAGE INTO THE BLOOD STREAM PROMOTION OF A NON IMMUNOLOGIC GUT DEFENSE BARRIER EFFICIENCY RELIABILITY R.O.I. Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013
  4. 4.  Single strains of PoultryStar® inhibit pathogenic bacteria  Enhancing effects through combination of strains PS®PS® PS® PS® PS®PS® PS® Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013
  5. 5. PROBIOTICS’ M.O.A. Immunity enhancement Improvement of the intestine’s immunologic barrier (Isolauri et al., 2001) Alleviation of intestinal inflammatory response (Isolauri et al., 2001) Gut stabilizing effect (Isolauri et al., 2001) (through balance control of proinflammatory and antiinflammatory Cytokines) (Isolauri et al., 2001) Heterophils stimulation (Farnell et al., 2006) Competitive exclusion Promotion of a nonimmunologic gut defense barrier (Isolauri et al., 2001) Normalization of increased intestinal permeability and altered gut microecology (Isolauri et al., 2001) Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013
  6. 6. Probiotics – Non immunological barrier formation Adapted Ng et al., (2009)
  7. 7. Probiotics – Immune response modulation Adapted Bron et al., (2011)
  8. 8. IL 10 IL 10 Copyright © BIOMIN GmbH Probiotics – Anti-inflammatory effect
  9. 9. Different steps… Probiotics – selection criteria ISOLATION OF BACTERIAL STRAINS • Isolation out of the chicken gut • Diff. selective and non-selective media • Diff. conditions (aerobic, fac. anaerobic, anaerobic) CHARACTERISATION OF BACTERIAL STRAINS • Physiologica and biochemical • Cultural-morphological • Molecular biological EVALUATION OF STRAINS • Pathogen inhibition • Adhesion to intestinal cell wall • Met. end products, stability, safety status
  10. 10. • From poultry to poultry • Chosen from different parts of the GIT. Species Origin Enterococcus faecium Jejunum Bifidobacterium animalis Ileum Lactobacillus salivarius Cecum Probiotics – different strains growing in different GIT tracts
  11. 11. THE TRIALS Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013
  12. 12. B.C.O. (Bacteria Chondronecrosis Osteomyelitis) LAMENESS  Worldwide surveys indicate the wide spread of lameness.  Studies in Europe show high level of lameness in broilers with 14-30% of the birds with lameness score >3 (Sanotra et al., 2003).  High prevalence rates up to 27.6% (UK- Knowles et al., 2008)  80% of the cases were attributed to BCO (Bacterial Chondronecrosis and Osteomyelitis) - 5% mortality.  10-15% of normal broilers suffer from subclinical BCO and the condition started to appear in younger birds (Thorp et al., 1993). ECONOMICAL LOSSES! Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013
  13. 13. Rapid growth rate Disruption of GIT barrier Joint trauma ex. Rough handling Immunosuppression ex. Mycotoxins, IBD, MD Inability of immune cells to reach the infection site Bone and joint infections Micro- fractures dysbacteriosis BCO Bacteraemia
  14. 14. Effect of PoultryStar® on incidence of BCO and lameness in commercial broilers reared on wire flooring University of Arkansas Division of Agriculture, Poultry Environmental Research Lab Poultry Research Farm, Fayetteville, Arkansas, USA Prof. R.F. Wideman, Jr., Ph.D. ________________________________________________ Wideman et al. (2012) A wire-flooring model for inducing lameness in broilers: Evaluation of probiotics as a prophylactic treatment, Poultry Science 91: 870 – 883
  15. 15. Trial design:  4 independent experiments  Location: Poultry Environmental Research Lab at the University of Arkansas Poultry Research Farm  Wire flooring model for inducing lameness in broilers Lameness trials - University of Arkansas, USA
  16. 16. Table: incidence % of lameness in broilers from two lines (C or D) that were fed control broiler starter feed (Control Feed) or the same feed containing PoultryStar® (Probiotic) while being reared on wood shavings litter or wire flooring from 1 through 56 days of age. a,b Values with different superscripts differed significantly at P < 0.05 using repeated Z-tests (SigmaPlot) to compare proportions. Experiment Line Gender Control Feed + Wood Shavings Total Lame Control Feed + Wire Flooring Total Lame PoultryStar® + Wire Flooring Total Lame 1 C M & F 12% b 68%a 36%b 2 D M & F 8%b 28%a 8%b 3 D M 2%b 22%a 10%ab 4 D M - 32%a 18%b Summary Results Exp 1-4 Lameness trials - University of Arkansas, US
  17. 17. Effect of PoultryStar® on amelioration of acute phase inflammation ____________________________ Purdue University (USA) Department of Animal Sciences
  18. 18. Trial design: 4 groups, 192 male broilers (8 replicates per group), injection of bacterial lipopolysaccharides (LPS) Negative control: no additive Positive control: LPS challenged (on days 14, 16, 18 & 20) Pair-fed group: non-challenged, pair-fed to the positive control PoultryStar® : microencapsulated, 2.0 x 108 CFU/kg of feed + LPS challenged Amelioration of acute phase inflammation Purdue University (USA)
  19. 19. Feed intake 2, 4 and 6 days after LPS challenge Amelioration of acute phase inflammation Purdue University (USA) Results -
  20. 20. Body weight gain from 14 (first day of LPS challenge) to 21 days Amelioration of acute phase inflammation Purdue University (USA) Results -
  21. 21. Effect of PoultryStar® on Clostridium perfringens-induced Necrotic Enteritis Ghent University (Belgium) Faculty of Veterinary Medicine Department of Pathology Bacteriology and Avian Diseases ____________________________________________________ M. Mohnl, F. Van Immerseel, R. Ducatelle2, G. Schatzmayr (2010) Inhibition of Clostridium Perfringens through Probiotic Strains and Efficacy of Multispecies Probiotic to Reduce Necrotic Enteritis in Poultry, XIIIth European Poultry Conference, Tours. France, 23-27 August 2010, Book of Abstracts, p. 254, oral presentation
  22. 22. Trial design Animals: day-old broiler chicks (mixed-sex; Ross) 3 groups, 90 birds, randomly assigend Negative control: uninfected, untreated Positive control: infected, untreated NC + PoultryStar® me: infected, 1 kg/ton of feed Feeding programm: Starter: day 1 to 8, wheat/rye and soybean based Grower: day 9 to 16, wheat/rye and soybean based Grower: from day 17, soybean meal was replaced by fishmeal Development of N.E. - Ghent University (Belgium)
  23. 23. Trial design • Gumboro vaccination on day 16 • Oral challenge (3 times a day) of infected groups with C. perfringens 56 (type A strain which carries netB; no beta-2 or enterotoxin genes) at days 17, 18, 19 and 20 • Oral gavage with a ten-fold dose of vaccinal Eimeria oocysts (Paracox-5) at day 18 • At days 22, 23, and 24, ten birds from each group were euthanized and intestinal lesions in the small intestine (duodenum to ileum) were scored Development of N.E. - Ghent University (Belgium)
  24. 24. Intestinal lesion scoring 0: no gross lesions 1: congested intestinal mucosa 2: small focal necrosis or ulceration (1 - 5 foci) 3: focal necrosis or ulceration (5 - 16 foci) 4: focal necrosis or ulceration (16 or more foci) 5: patches of necrosis 2 - 3 cm long 6: diffuse necrosis typical of field cases Lesion scores of 2 or more were classified as necrotic enteritis positive. The statistical analysis was carried out for the 3 sampling days (total) by the means of multivariable logistic regression. Development of N.E. - Ghent University (Belgium)
  25. 25. Number of birds with NE lesions per sampling day Results Development of N.E. - Ghent University (Belgium)
  26. 26. APPLICATION OF PROBIOTICS Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013
  27. 27. Naturally ahead 21 days 42 days1 day Day 1: Marek IB NDV Coccidiosis? Day 14: IBDV (1-2?) Day 18: IB variant NDV 10 days FEED APPLICATIONPoultryStar® ME (1 kg/ton) FEED CHANGE FEED CHANGE PoultryStar® ME (500 g/ton) IN CASE OF SEVERE LAMENESS COMP. EXCLUSION Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013 PoultryStar® ME (500 g/ton)
  28. 28. YES Antibiotics - Probiotics compatibility?
  29. 29. (Ouwehand et al. 2000; Rachmilewitz et al. 2004; Katakura et al. 2005; Lorenzoni et al., 2012 Rehman et al., 2012). Antibiotics - Probiotics compatibility?
  30. 30. Naturally ahead TAKE-HOME MESSAGES • Probiotics can enhance immunity • Probiotics can create a non immune defensive barrier in the GIT • Multi-specie probiotics are more efficient than single-strain ones (they can colonize the whole GIT) • Specie-specific probiotics can colonize better the GIT and stay longer in the GIT after stopping the administration • Multi-specie, specie-specific probiotics can give a benefit in terms of BCO lameness reduction Acute-phase inflammation modulation, N.E. reduction Probiotics in poultry nutrition: cost or opportunity? BAROMFI CONGRESS 2013
  31. 31. PoultryStar® Healthy gut - Strong chick!
  32. 32. • Poultry specific Out of poultry for poultry • Multi-species • Synbiotic Synergistic combination of probiotics and prebiotics PoultryStar® is… • Microencapsulated Heat-stable, strain-tailored microencapsulation • Scientifically proven benefits Worldwide R&D cooperation Enterococcus faecium 60% Bifidobacterium animalis 30% Lactobacillus salivarius 10%
  33. 33. PoultryStar® ME - feed application Can be pelleted up to temperatures of 85°C x 20 sec Packaging: 25 kg corrugated cartons with polyethylene inlet Contains 2x108 CFU/g Dosage: 0.5 - 1 kg/ton of feed PoultryStar® - Healthy gut, strong chick!
  34. 34. Origin chicken intestine ✓ ✓ ✓ ✓ Inhibition of pathogens ✓ ✓ ✓ ✓ Adhesion to intestinal cell walls ✓ ✓ ✓ ✓ Acidification of gut (lactic acid:1, acetic acid: 2) ✓ 1 ✓1,2 ✓1 ✓ Production of antimicrobial sustances ✓ ✓ ✓ ✓ Stability (storage, bile salts, acids) ✓ ✓ ✓ ✓ Safety ✓ ✓ ✓ ✓ PoultryStar® - Healthy gut, strong chick! PoultryStar®ME Lactobacillussalivarius Bifidobacterium animalis Enterococcusfaecium
  35. 35. PoultryStar® - Healthy gut, strong chick! So… Probiotics can be just a cost or an opprtunity? CATCH IT!!

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