Your SlideShare is downloading. ×
Francesco prandini
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Francesco prandini

436
views

Published on

Published in: Health & Medicine, Technology

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
436
On Slideshare
0
From Embeds
0
Number of Embeds
1
Actions
Shares
0
Downloads
3
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. Francesco Prandini – DVM MERIAL Avian Technical Manager EMEA Protection against Salmonella infection using vaccines Baromfiágazat annual Seminar 2013 Budapest, October 1st 2013
  • 2. Introduction • Salmonella spp is one of the major causes of food-borne illness in humans • High prevalence of Salmonella in poultry • Chicken and chicken products are the most frequent sources of zoonoses World Poultry - Dec 29, 2011
  • 3. Salmonella • Motile non-host-specific serovars: >2000 serovars nearly ubiquitous in wild and domestic animals • Very common but seldom cause clinical disease • More often, asymptomatic persistent colonization of the intestinal tract • “Paratyphoid” infections of poultry and zoonoses
  • 4. Zoonoses cases in humans in 2011
  • 5. Salmonella epidemiology • S.Enteritidis (SE) and S.Typhimurium (ST) are the serovars most frequently associated with salmonellosis in humans through the food chain • Commonly asymptomatic colonization of chickens in the intestinal tract • Egg shell contamination • SE can infect eggs internally
  • 6. Distribution of food vehicles in outbreaks caused by S.Enteritidis in the EU, 2011
  • 7. Distribution of the 10 most common Salmonella serovars in humans in the EU, 2010-2011
  • 8. Salmonella in human cases, eggs and laying hens and the number of Salmonella outbreaks caused by eggs within the EU, 2007-2011
  • 9. Breeders Gallus gallus Laying hens Gallus gallus Broilers Turkeys S.Enteritidis S.Enteritidis S.Enteritidis S.Enteritidis S.Typhimurium S.Typhimurium S.Typhimurium S.Typhimurium S.Infantis *All salmonella serovars with public health significanceS.Hadar S.Virchow Serovars* with established targets for reduction of prevalence in the EU
  • 10. Salmonella control in Chickens • Control of biological reservoirs: – Breeders – Replacement flocks – Rodents – Red mites • Rigorous biosecurity measures • Slaughtering of positive Breeders/Layers flocks • Competitive exclusion and Probiotic effects • Vaccination • Comprehensive programs of risk reduction
  • 11. Salmonella VACCINATION Specific protection against S. serovars Both Live and Killed vaccines have been associated with significant protection An important component of SE-ST plans of risk reduction in Layers and Breeders Farm-tailored vaccination strategies
  • 12. Salmonella VACCINES Live attenuated vaccines: • Mass application • Mainly local immune response Inactivated vaccines: • Individual s.c./i.m. injection • Mainly humoral immune response Vaccination programs: • Live • Inactivated • Live + Inactivated
  • 13. Live VACCINE  A live attenuated vaccine against S. Enteritidis  Adenine - Histidine double auxotrophic strain  Drinking water administration  2 or 3 doses tailored programs  Early and long-lasting protection  Easy and rapid field strains differentiation Gallivac Se strain Field strain
  • 14. Challenge trial with GALLIVACChallenge trial with GALLIVAC®® SeSe Oral Vaccination @ 2,16 & 114 days of age, and challenge with 5 billion pathogenic SE bacteria into the crop of each bird at 78 weeks of age. Number of bacteria per gram of tissue 7 days after challenge. 0 10.000.000 20.000.000 30.000.000 40.000.000 Caecum @ 79 weeks Un vaccinated Gallivac Se 0 50 100 150 200 250 300 Liver at 79 weeks
  • 15. Inactivated VACCINE  A bivalent inactivated vaccine against S.Enteritidis and S.Typhimurium for pullets  Innovative manufacturing method to preserve antigenicity  0.3 ml emulsion dose s.c. or i.m.  2 doses program: from 6 wks & 16 wks  Rapid and lasting protection Classical processControlled nutrient fermentation
  • 16. Blood sampling Comparison of inactivated vaccines Gallimune® Se+St W10 W16 W16 W10 W30 Se+St Al hydroxide W16W10 Control group unvaccinated W30 W30 G 1 G 2 G 3
  • 17. Antibody titres at 30 weeks
  • 18. Gallimune® Se+St W10 Competitor vaccine W16 W16 W12 Challenge W27 W27 +4 days +4 days Ovary sampling 60 pullets SE PT4 oral challenge Challenge 1: ovary contamination
  • 19. Challenge trial with Gallimune® Se + St Results of isolation of S. Enteritidis PT4 in the ovaries of birds 4 days post oral challenge at 27 weeks of age. -90%
  • 20. • Groups of 60 pullets: – Vaccinated at 10 & 16 weeks of age with Gallimune Se + St - 0.3 ml – 12 & 16 weeks with reference vaccine - 0.5 ml – Controls – 29 weeks of age: oral challenge with ST – 31 weeks of age: oral challenge with SE – Caecal droppings collected 4 days post challenge Challenge trial with Gallimune® Se + St
  • 21. Gallimune ® Se+St W10 W16 W16 W12 W29 W29 +4 days Challenge 2: fecal shedding Other vaccine • 29 weeks  ST challenge oral route • 31 weeks  SE challenge oral route +4 days Gallimune® Se+St Reference vaccine Challenge Faeces sampling
  • 22. Challenge 2: fecal shedding
  • 23. Challenge 2: fecal shedding
  • 24. SINGLE or COMBO vaccination programs PROTOCOL Age of chicks Group 1 Group 2 Group 3 Day 1 Gallivac Se drinking water Gallivac Se drinking water Controls Day 42 Gallivac Se drinking water Gallivac Se drinking water --- Week12 Gallivac Se in drinking water --- --- Week 4 Gallimune Se+St im breast injection --- Week 52 challenge with SE or ST challenge with SE or ST challenge with SE or ST
  • 25. Challenge study PROTOCOL • Lohmann Brown Commercial layers • Oral challenge with 109 CFU of SE or ST • Birds sacrificed 7 days after challenge • Enumeration of SE and ST cells per g. of caecal content
  • 26. 52 weeks - SE challenge
  • 27. 52 weeks - ST challenge
  • 28. Conclusions  Salmonella requires comprehensive control plans  Vaccination is an important component  Live and inactivated vaccines has specific features  Combined vaccination programs consolidate advantages from both  Farm-tailored vaccination strategies are required
  • 29. Thanks for your attention