Normal red cells (upper image). Notice the “doughnout-like” shape of the erythrocytes, with the thickness of the coloured peripheral area being approximately equal to that of the discoloured central area.
Hypochromic red cells (lower image). Notice the thin, ring-like coloured area of the severly hypochromic erythrocytes (anulocytes). This aspect is typical for iron deficiency anemia (IDA).
The typical small, fully coloured (no central discoloured area) spherical red cells (blue arrows) on the peripheral smear (upper image).
Persistence of some microspherocytes and the presence of Howell-Jolly bodies (small, round, purple inclusions in erythrocyte cytoplasm) in a patient with hereditary microspherocytosis after splenectomy (lower image).
Howell-Jolly bodies (red arrow) are small remnant DNA inclusions in erythrocyte cytoplasm. These inclusions are normally pitted out by the spleen during erythrocyte circulation, but will persist in individuals with functional or anatomic asplenia.
“ Target” cells (red arrow) and basophilic stippling (small, multiple purple iclusions in erythrocyte cytoplasm, green arrow) with mild microcytosis and hypochromia in a case of minor beta-thalassemia (upper slide).
Microcytosis, hypochromia, target cells, erythroblastosis and Howell-Jolly bodies in a case of splenectomized beta thalassemia major (lower slide).