IMMUNOHISTOCHEMICAL EXAMINATION OF THE LEVEL OF NEUROENDOCRINE DIFFERENTIATION IN COLORECTAL ADENOCARCINOMA <br />Mentor: ...
IntroductionAim Method Results Conclusion<br />Colorectal cancer is the third most <br />frequent cancer in both sexes and...
Introduction Aim Method Results Conclusion  <br />Most of neuroendocrine cells in colon mucosa are localized in distal thi...
Introduction Aim Method Results Conclusion <br />Detection of neuroendocrine fields of small cell carcinoma appearance whi...
Hypothesis<br />There are numerous cells of endocrine features in the parenchyma of colorectal carcinoma. <br />Number of ...
IntroductionAim Method Results Conclusion<br />Analysis of neuroendocrine cells participation in colorectal carcinoma. <br...
Introduction Aim Method Results Conclusion<br /><ul><li>Study encompassed 90 patients with sporadic type of colorectal car...
Patients  did not receive preoperative chemotherapy or radiotherapy.
All patients in the study were involved regardless of their sex, age or stage of disease.
Data for all patients were obtained from case histories of disease, patohistological referrals and oncological records. </...
 The stage of disease
Histological and nuclear tumour grade
Type of invasive growth</li></li></ul><li>            IntroductionAim Method Results Conclusion<br />The stage of tumour d...
Group B – patients at the II stage of tumour disease;
Group C – patients at the III stage of disease and
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Immunohistochemical examination of the level of neuroendocrine differentiation

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Immunohistochemical examination of the level of neuroendocrine differentiation

  1. 1. IMMUNOHISTOCHEMICAL EXAMINATION OF THE LEVEL OF NEUROENDOCRINE DIFFERENTIATION IN COLORECTAL ADENOCARCINOMA <br />Mentor: M.Sc. MD DanjelaBatinicSkipina<br />Authors: NenadTodorovic<br />Snezana Milosevic<br />
  2. 2. IntroductionAim Method Results Conclusion<br />Colorectal cancer is the third most <br />frequent cancer in both sexes and the second leading cause of malignant diseases-related death in developed countries. <br />Neuroendocrine cells which develop from stem endodermal cells represent 2% of the total number of epithelial colon cells. <br />
  3. 3. Introduction Aim Method Results Conclusion <br />Most of neuroendocrine cells in colon mucosa are localized in distal third of Lieberhühn’s crypt.<br />A great number of <br />neuroendocrine cells are <br />registered in benign tumours <br />(adenoma) <br />and in the ulcerative colitis as <br />precancerous state of colon. <br />
  4. 4. Introduction Aim Method Results Conclusion <br />Detection of neuroendocrine fields of small cell carcinoma appearance which determines the biological behaviour of <br />adenocarcinoma has a special<br />significance .<br />A great number of <br />neuroendocrine cells is <br />detected on the inavsive edge<br />of tumour and on the <br />transitional mucosa. <br />
  5. 5. Hypothesis<br />There are numerous cells of endocrine features in the parenchyma of colorectal carcinoma. <br />Number of neuroendocrine cells in adenocarcinoma is in correlation with the stage of disease and other patohistological parametres of unfavourable prognosis. <br />
  6. 6. IntroductionAim Method Results Conclusion<br />Analysis of neuroendocrine cells participation in colorectal carcinoma. <br />Analysis of correlation between neuroendocrine differentiation level and the stage of disease and other patohistological characteristics of colorectal adenocarcinoma. <br /> <br />
  7. 7. Introduction Aim Method Results Conclusion<br /><ul><li>Study encompassed 90 patients with sporadic type of colorectal carcinoma in which the radical surgical operation was perfomed.
  8. 8. Patients did not receive preoperative chemotherapy or radiotherapy.
  9. 9. All patients in the study were involved regardless of their sex, age or stage of disease.
  10. 10. Data for all patients were obtained from case histories of disease, patohistological referrals and oncological records. </li></li></ul><li>IntroductionAim Method Results Conclusion<br />Representative samples of non necrotic tumour tissue were fixed in 10% formalin and embedded into paraffin blocks. Deparaffined tissue 3-5 µm thick sections were stained with Hematoxylin-Eosin method. Using pato-anatomic and patohistological analyses, we determined:<br /><ul><li>Macroscopical and histological type of colorectal carcinoma
  11. 11. The stage of disease
  12. 12. Histological and nuclear tumour grade
  13. 13. Type of invasive growth</li></li></ul><li> IntroductionAim Method Results Conclusion<br />The stage of tumour disease was determined according to TNM classification. The patients were divided in accordance with TNM classification:<br /><ul><li>Group A – patients with colorectal cancer at stages 0 and I;
  14. 14. Group B – patients at the II stage of tumour disease;
  15. 15. Group C – patients at the III stage of disease and
  16. 16. Group D – patients at the IV stage of disease. </li></li></ul><li> IntroductionAim Method Results Conclusion<br />According to the percentage of tumour cells which showed positive immunohistochemical reaction, the samples were divided into three groups:<br /><ul><li> Group 1- there were no cells in the tumour which showed expression of chromogranin A;
  17. 17. Group 2 – less than 10% of tumour cells showed positive immunohistochemical reaction;
  18. 18. Group 3 – more than 10% of tumour cells showed positive immunohistochemical reaction. </li></li></ul><li>IntroductionAim Method Results Conclusion<br />Figure 1: Distribution of expression in relation to the number of patients.<br />
  19. 19. IntroductionAim Method Results Conclusion<br />Fugure 2: Distribution of expression in relation to the stage of disease.<br />
  20. 20. IntroductionAim Method Results Conclusion<br />Figure 3: Distribution of expression in relation to the T stage of disease:<br />
  21. 21. IntroductionAim Method Results Conclusion<br /><ul><li>There is a statistically significant difference in the expression CHR in patients surgically treated at II and III stage of disease and stages T3a/b and T3c/d, but not at the stages T2 and T3 of locally advanced disease.
  22. 22.  There is a statistically significant positive correlation between expresion CHR and the stage of disease and the number of lymph nodules with metastases.</li></li></ul><li>THANK YOU FOR YOUR ATTENTION !!!<br />

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