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Acidfaststaining 090911062148 Phpapp02
 

Acidfaststaining 090911062148 Phpapp02

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    Acidfaststaining 090911062148 Phpapp02 Acidfaststaining 090911062148 Phpapp02 Presentation Transcript

    • Acid fast stainingsummary
      Microbiology Lab 11th Sept
      Ravi A Patel
    • 2 procedures for acid fast staining
      ZiehlNeelsen’s Method (Hot Method)
      ------The one presented as Demo
      Kinyoun’s Method (Cold Method)
      ------The one which we performed today
    • Special structures we saw
      Flagella
      Capsule
      Metachromatic granules
      Spores
    • Smear on the slide made from sputum of the patient having tb
    • ZIEHL NEELSEN’S METHOD-hot method
      +VE & -VE ACID FAST STAINING------? What does it mean
      ------If the Organism does not take the counter stain color(In this case Methylene blue) and sustains its primary stain color(Red) then it’s a +ve acid fast staining
      And Vice Versa…………..for –ve Acid Fast staining
      * Involves heating for 5 min on slow burner after being stained with primary stain so called Hot Method unlike the Kinyoun’s in which we did not use burner after primary staining…..
    • Ziehlneelsen’s method
      In the slide we saw the Red colored +ve Acid fast stained Bacilli although the blue colored background was not so prominent..
    • Kinyoun’s method
      Group-9 got a –ve acid fast staining bacilli
      Group-2,3,7 got +ve Acid fast staining Bacilli
      This picture is of +ve Acid fast staining bacilli
      If we look
      closely at the
      pointer there
      is a red bacilli
      over green
      background
      of malachite
      green
    • Special structures
    • flagella
    • flagella
      Stained by :- Liefson’s stain
      Function :- Locomotion
      Composition :- Protein Flagellin
      Types :- Mono-Amphi-Lopho-Peri-Atrichous
      ( Acronymn- P-A-A-L-M )
    • capsule
      Structure at the pointer capsule and within capsule we see mycobacterium
    • Capsule
      Stained by :- Hiss stain
      Function :- Antiphagocytosis
      Protects the bacteria(Virulence of the bacteria)
      Charateristic:- Antigenic
    • Struct. At the pointerMetachromatic granules
    • Metachromatic granules
      Stained by :- L.A.M.B stain
      (loeffler’s Alkaline methylene blue)
      Function:- Temporarily stores metabolites(carbon, phosphates)
    • Stucture at the pointer spores
    • Spores(endospores)
      Stained by:- Dorner stain
      Function:- Provides resistance to bacteria in adverse conditions
      Characteristic:- Spores are dormant cells
      They can undergo germination
    • Thanking to the entire universe